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处死

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The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清,进行以下检测:(1)原子吸收法测血清锌浓度;(2)测胸腺、脾脏脏器重量并与大鼠体重比较,计算脏器指数;(3)HE染色对胸腺、脾脏进行病理观察;(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡,并通过图像分析进行比较;(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达;(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达;(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

After killing the mice, weights of thymuses and spleens, and the macrophage phagocytosis were detected. using sheep red blood cells as antigen, the amounts of antibody-producing cells in mice were counted. through lymphocyte conversion test, lymphocyte conversion ratios of spleen lymphocytes stimulated by cona in mice were determined.

处死后检测小鼠胸腺重、脾重、巨噬细胞吞噬功能;采用绵羊红细胞作为抗原,检测小鼠抗体生成细胞功能;采用mtt法检测小鼠脾淋巴细胞对cona刺激的转化能力。

If the owner do not bring witnesses to identify the lost article, he is an evil-doer, he has traduced, and shall be put to death.

若遗失物所有人没有提供能识别失物的证人,那么他就是中伤他人的诽谤者,应被处死

Methods Adult male waster rats were randomly divided into the normal control pseudo-group and the experimental operation-group. In the operation-group, the right sciatic nerve was transected and survived for 1, 2, 4, 6 and 8 weeks after operation.

选取健康成年雄性Wister大鼠80只,随机分为假手术组和坐骨神经切断组,分别于术后1、2、4、6、8周五个时相处死后取患侧L4-L6背根神经节,应用Western Blot和免疫组织化学技术检测P-ERK1/2在背根神经节中的表达变化。

All mice were killed by cutting neck on 7th day,ulnas and femur were taken out.The bone marrow histologic morphology and ultrastructure of mice ulnas were ovserved.

于第7天断颈处死小鼠,取出尺骨和股骨,计数骨髓单个核细胞,并行尺骨组织学切片和超微结构观察。

The Mother upbraided him as an unnatural child, whereupon he replied,"If you had beaten me when I first stole and brought to you that lesson--book, I should not have come to this, nor have been thus led to a disgraceful death."

母亲责备他是个不孝的儿子,他回答说:&如果我第一次把教科书偷回家时,你打我一顿,我就不会变成这样,也不会落得个不光彩地被处死的下场。&

In addition, the document says, he was "led to believe he was going to be executed, and urinated on himself," and was told that his mother and family would be detained and harmed.

此外,该文件说,他领导的&相信他是要被处死,并urinated对自己&,有人告诉他的母亲和家庭将被拘留和伤害。

Animals in injury group were subjected to a m oderate lateral hydraulic percussion brain injury (0 .2 MPa),and then were subdi vided into 5,15,30,60,120,240,480 and 720 minutes groups according to the tim e elapsed after injury .

损伤组 动物均给以0 。2 MPa液压冲击脑损伤,按冲击后处死时间不同再分为5、15、30、60、120、 240、480和720分钟组。

METHODS: All rats were killed following 4 weeks of feeding, the ascending aorta were harvested and preserved at -20 ℃ refrigerator.

两组大鼠饲养4周后处死,取出大鼠升主动脉,密封后置于-20 ℃冰箱内保存备用。

Photos: Haitian robber was killed Adam Street naked whipped ...10-01-13 * Gong Guanghai so many times in Hainan murder was sentenced to death ; 09-12-14 * Bank Monitoring photographed woman was attacked by gunmen scenario; 09-12-11 * Guangdong A level ...

组图:海地抢劫者被当街裸体鞭打处死。。。。 10-01-13·多次在海南谋财害命巩广海等一审被判处死刑; 09-12-14·银行监控拍下女子遭持枪歹徒抢劫情景; 09-12-11·广东A级。。。

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