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Digital substraction angiography was performed 1 and 4 weeks after operation to observe the vessel changes, and then all canines were sacrificed for histological examination.

术后1周和4周行数字减影血管造影观察血管情况;4周后处死动物,行溃疡周围组织学检查。

And 1‰ test groups were fed with 0.4‰, 0.8‰, 1‰ formaldehyde sulphoxylate solution. In the 10 and 30 day, the mice were killed, the cerebrum tissuce were took out, and fixed in the Zamboni fixation solution, conventionally produced frozen section and stained by immunohistochemistry.

对照组小鼠喂食静置自来水;实验组小鼠分别代以0.4‰、0.8‰、1‰次硫酸氢钠甲醛溶液喂食,10d和30d时分别处死各组小鼠10只,取出大脑,Zamboni固定液固定,常规冰冻制片,免疫组织化学染色,光镜观察。

METHODS: ADSCs were prepared by collagenase I digestion of subcutaneous fat from the suprascapular site of Japanese white rabbit.

1.2.1 兔ADSCs(rabbit ADSCs, rADSCs)的原代培养 3 mo龄成年日本大耳白兔,耳缘静脉注射空气处死

Then killed the animals and extracted hippocampus.The serum of TChE,SOD CAT and MDA were detected.

然后将各组剩余小鼠断髓处死,冰台上迅速取出脑组织,在预冷的生理盐水中漂洗,除去血液,分离出两侧的海马,投入4℃冰浴的玻璃匀浆器中,按1∶9于生理盐水中制成10%脑组织匀浆,在4℃下4000r/min离心10min,取上清液以检测小鼠海马组织中乙酰胆碱酯酶、超氧化物歧化酶、过氧化氢酶的活力和丙二醛的含量。

After 5 days we began to measure the abilities of learning and memory in the Morris water maze. The avoiding latency, the frequency of passing the platform and the stay time in the aim quadrant were determine in mice. The activities of TchE and MAO in the cerebra of mice were detected.

第5d后进行Morris水迷宫测试,测试期间保持音乐刺激不变,放完音乐后测试潜伏期、经过平台次数和平台象限停留时间,实验结束后处死小鼠取脑组织并匀浆,检测乙酰胆碱酯酶活性和单胺氧化酶活性。

Rabbits were sacrificed at 12 weeks.The maximum loading of the femur-graft-tibia complexes were determined in 858 Mini BionixⅡtestor,and the rupture site was observed.

于术后12周处死动物,在858 Mini BionixⅡ型生物力学测试机测定股骨-移植物-胫骨的复合极限载荷、断裂部位。

To detect the level of blood lipid, endodermis tetrachoric and hs-CRP.Then to observe the changes of pathomorphism with light microscope by fixing and staining to aorta pectoralis after sacrificing rats.

检测大鼠血脂、内皮四项,处死后取出胸主动脉固定、染色后,光镜下观察病理形态学改变;2。

Now this painting stood under the great high arches of the palace gates somewhere over there. And a soldier put up a ladder to go and get the painting and somebody rushed out of the crowd and pushed the ladder down and killed the soldier. But the other soldiers took the painting down and arrested the person who had killed their friend and they had her executed. And Saint Theodosia the virgin martyr became the first victim of iconoclasm.

这幅画位于宫殿的大拱门下,就在那里的某处,一位士兵爬上梯子拆画像,有个人从群众当中冲出来推倒梯子,士兵倒地而死,但其他士兵还是拆下了画像,逮捕害死他们同伴的人,并将她处死,殉教士圣西奥多西娅成为第一个捣毁圣像的牺牲者。

If any one steal cattle or sheep, or an ass, or a pig or a goat, if it belong to a god or to the court, the thief shall pay thirtyfold therefor; if they belonged to a freed man of the king he shall pay tenfold; if the thief has nothing with which to pay he shall be put to death.

任何人偷了供奉给神的或者属于宫廷的牲畜,应被处以三十倍的罚金;若被偷的牲畜属于穆什钦努,此人应被处以十倍的罚金;若此人没有任何财产可以支付这些罚金,应被处死

The rabbits were sacrificed at 5,10 and 15 weeks. The fixed bone segments of both tibiae were removed for radiological and histological examination.

术后5、10、15周分三批处死动物后测量骨折和螺钉间内、外径和骨皮质厚度并进行光镜和荧光观察。

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