处死

Five sheep were sacrificed at 3, 4 and 6 weeks after osteotomy, respectively, and specimens were harvested for detecting the expression of TGF-β1 and IGF-I by immunohistochemistry and RT-PCR.

术后3、4和6周分别处死5只动物，应用免疫组织化学与RT—PCR检测骨痂组织中TGF—β1与IGF—Ⅰ的表达。

NAFLD rat model was established by feeding with high caloric diet. Serum TC, TG, ALT, AST and liver lipid contents, oxidoreduction index ,liver histology were assayed at the end of the 18th week.

以高脂饲料饲养诱发脂肪肝大鼠模型。18周末，处死所有动物，检测血脂、肝功能、肝脂质含量、氧化还原指标、肝组织病理学等。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

Methods: To establish a rat model of pancreaticoduodenal transplantation with venacava endocrine drainage and enteric eccrine drainage. The recipient rats were introperitoneally injected with 15 mg/kg of proline dithiocarbamate in experiment group and with equal amount of normal saline in control group 30 minutes before transplantation.

建立大鼠腔静脉内分泌引流、肠道外分泌引流的动物模型，脯氨酸二硫代氨基甲酸酯组供体胰腺保存在ProDTC的UW液中，受体鼠于移植前30min腹腔注射ProDTC(15mg/kg)；对照组供体胰腺仅保存在UW液中，受体鼠腹腔注射等量生理盐水，分别于再灌注后第0、1、3、6、12、24h等时点，处死大鼠，取血后切取移植胰腺。

After seven-days nutritional supporting,the rats\' weight change and ascitic fluid was recorded,serum biochemistry index,pancreatic enzyme index, and malonaldehyde concentration were examined,and the pancreatitic tissue were stained with hematoxylin eosin,and histopathologically scored.

营养支持7天后处死大鼠，记录大鼠体重变化及腹水情况，检测大鼠血清生化酶学指标及胰腺酶学指标、丙二醛含量，并将胰腺标本行苏木精伊红染色进行组织病理评分。

The results of behavioral scoring for each animal demonstrated one animal with paraparesis and other 5 animals with paraplegia.

复苏后1只动物术后每天肌力均为IV 级，7天后处死时肌力IV～V级；其余5只动物术后下肢肌力0 ～I级。

The results of behavioral scoring for each animal demonstrated one animal with paraparesis and other 5 animals with paraplegia.

复苏后1只动物术后天天肌力均为IV 级，7天后处死时肌力IV～V级；其余5只动物术后下肢肌力0 ～I级。

It is of course obvious that , since murderers who would have previously been executed will now be sentenced to imprisonment as a result of the Homicide Act. a new consideration will have to be given to the question of the length of imprisonment which can be imposed consistently with our general penological notions.

14[13]当然，很明显，因为以前会被处死的谋杀犯，作为杀人法的结果，现在会被判处监禁，所以，我们必须重新考虑能被与我们的一般的刑罚学观念相一致地适用的监禁的期限的问题。

Microbubbles attached with the naked plasmid DNA of EGFP were infused into the rat by periocular injection with destructing microbubbles by ultrasound immediately. After 2 weeks , the EGFP expression in the rats'retina were detected by confocal laser scanning microscopy .

两周后处死大鼠做冰冻切片，在激光共聚焦显微镜下观察蛋白表达情况及分析EGFP质粒在大鼠色素上皮层的表达强度。

Histological changes of periostea on the mandibular ramus after the periosteal distraction:2.1 20 adult New Zealand rabbits were operated and distracted with the same methods mentioned above.2.2 Every 5 animals were killed postoperatively at days 28,35,42 and 56 respectively.

2.2、术后第28、35、42和56天分别处死5只实验兔，每组随机选其中2只取实验侧和对照侧的下颌升支骨膜进行HE染色观察；每组3只实验兔取实验侧和对照侧的下颌升支骨膜进行增殖细胞核抗原免疫组织化学染色观察。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。