处死

Renal tissue were examined by light microscopy at 12 weeks after operation. Immunohistochemistry and in - situ hybridization was applied to measure the expression of CTGF and CTGF mRNA in the nephridial tissue.

实验12周处死大鼠，用免疫组化法观察CTGF表达及RT-PCR法测肾皮质CTGFmRNA表达水平。

The nephridial tissue of all the rats was taken for pathological examinations.

选用健康雄性SD(Sprague-Dawley)大鼠，腹腔注射8 mL/kg的DEG，于注射前和注射后第2天、5天、7天以及14天尾静脉采血检测血肌酐、尿素氮和CO2结合率(CO2CP)，收集死亡大鼠及第7天和14天处死大鼠的肾组织进行病理检查。

Healthy SD male rats were given DEG with the dose of 8 mL/kg body weight (intraperitoneal injection I.P.). The level of the serum Cr, BUN and CO2CP(CO2 Combining Power CO2CP)were tested before and after the injection the 2nd , 5th , 7th, and 14th day. The nephridial tissue of all the rats was taken for pathological examinations.

选用健康雄性SD(Sprague-Dawley)大鼠，腹腔注射8 mL/kg的DEG，于注射前和注射后第2天、5天、7天以及14天尾静脉采血检测血肌酐、尿素氮和CO2结合率(CO2CP)，收集死亡大鼠及第7天和14天处死大鼠的肾组织进行病理检查。

The results showed that in the control and experimental groups, HMGB1 immunoreactivity was all diffusely distributed in the cytoplasm of neuroepithelial cells and mesenchymal cells surrounded the neural tube at 8,16 and 24 hours after water bath treatment.

将孕鼠随机分为实验组和对照组，分别于水浴处理后8、16、24、40、64 h（相当于胚龄第8、8.5、9、9.5、10.5 d）处死孕鼠，剖腹取鼠胚，制备石蜡切片，应用免疫荧光染色技术检测NTDs发生过程中HMGB1在神经上皮细胞中的表达变化。

Bone graft incorporation and histomorphometery were evaluated in nondecalcified section.

处死前2周和1周时，经胫前皮下静脉注射四环素溶液(30mg/kg)进行荧光标记。

Result The feeding and hygiene management methods of the Ochotona Curzoniae were gradually established. We also developed 11 kinds of basic methods and techniques,such as animals crawling、marking、fixing、anesthesia、drug administration、executing and so on.

结果 建立了高原鼠兔饲养管理和卫生管理方法，建立抓取、标记、固定、麻醉、给药、取材、处死等11种动物实验基本方法和技术。

The histological structures of their uteri were analysed. Results\ Oestrus\|related changes in the histological structure of uterus and weight were observed.

我们在已往的研究工作中发现，雌性实验动物的子宫组织形态和子宫重量个体差异很大，即使是同年龄相同时间处死的受试动物也不例外，尤以雌性毕格狗表现更为明显。

ICH was induced by the injection of 50 ul autologous blood into the right caudate nucleus, while 50 μl natural saline was injected in the control group. The rats were killed after 6h, 24h, 2d, 3d, 5d and 7d respectively, the morphology changes of nerve cell oncosis and count by electron microscope and the immunohistochemical method and computer image analysis were performed in rats brain by using antibody of VEGF.

采用二步法制备大鼠脑出血模型，脑出血组注入自体血50μl，对照组注入等量生理盐水，分别于6、24h、2、3、5、7d后处死，行HE染色和免疫组化染色观察脑内VEGF表达变化；电镜下观察胀亡细胞形态学变化并计数。

All rats were fed under the same conditions for 7 days and sacrificed. Bilateral orchidectomy was performed and germ cell apoptosis was detected.

按Turner法建立睾丸扭转／复位模型，所有大鼠均在同等条件下喂养至术后7 d处死，切取双侧睾丸后检测凋亡指数。

The capacity of repairing bone defect of biodegradable PLA and the efficacy of PLA as a carrier for bone morphogenetic protein are determined with detecting the osteogenetic process.

18只日本大耳白兔，随机分组，在双侧下颌骨体部形成 12 m m× 6 mm的缺损，分别植入聚乳酸-人骨形成蛋白- 2复合物(PL A- rh BMP- 2 )、单纯聚乳酸，于 2、4、8、12周分批处死。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。