处死

Method］Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum, which were fixed with ATMFS or steel plate respectively. The animals were sacrificed and specimens were procured at 2,4,6,8 and 12 weeks after operation.

］选用10只成年杂种家犬，双侧髋臼臼顶上方1.5 cm处横形截骨，分别采用ATMFS(骨盆髋臼三维记忆内固定系统)前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周处死动物取材，行HE染色、Masson三色法染色组织学检查，图像分析新骨形成积分光密度值，研究不同应力作用模式下的骨盆骨折愈合情况。

［Objective］To explore the effect of continuous physiological osteogenic stress on the bone healing of canine iliopectineal crest.［Method］Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum,which were fixed with ATMFS or steel plate respectively. The animals were sacrificed and specimens were procured at 2,4,6,8 and 12 weeks after operation.Samples from the fracture gaps were investigated by histology of HE and Masson staining,image pattern analysis of new bone formation.

探讨持续顺应生理力线的压应力对犬骨盆弓状线部骨折愈合的影响［方法］选用10只成年杂家犬，双侧髋臼臼顶上方1.5 cm处横形截骨，分别采用ATMFS(骨盆髋臼三维记忆内固定系统)柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周处死动物取材，行HE染色、Masson三色法染色组织学检查，图像分析新骨形成积分光密度值，研究不同应力作用模式下的骨盆骨折愈合情况。

To travel guides, Chindia is a place in Romania where Vlad the Impaler, a bloodthirsty 15th-century monarch who inspired the fictional Count Dracula, watched his victims put to death.

在旅游指南中，Chindia是罗马尼亚一处地名，15世纪时曾出过一位嗜血暴君&穿刺王弗拉德&，他喜欢看着那些受害者被处死，后来成为小说《吸血鬼德古拉伯爵》的创作灵感来源。

MATERIALS AND METHODS: hMSCs labeled with enhanced green fluorescent protein were transplanted into the lateral ventricle of neonatal mouse brain. At 0, 9 and 14 days post-transplantation, MICE were sacrificed and their brains were fixed with 4% paraformaldehyde. The engraftment of transplanted cells in coronal section of the recipient mouse brain was examined wider a fluorescent microscope. Indirected immunofluorescent staining was used to detect the expression of neural proteins of these grafted cells.

材料与方法：将标记绿色荧光蛋白的人骨髓间充质于细胞植入到新生3d的小鼠侧脑室中，分别于移植后0d，9d和14d处死受休鼠，取其脑组织，4%多聚甲醛固定后行冠状面冰冻切片，荧光显微镜下观察hMSCs的植入，激光共聚焦显微镜检测植入细胞神经特异性蛋白的表达，定位双重标记的植入细胞。

Their different part of denture were ground artificially to establish an animal model of infraocclusion.

采用人工磨低大鼠不同牙列段牙齿的方法建立低位咬合动物模型，于干预后1，2，4wk后将各组分别处死大鼠4只。

Methods Twenty-four Sprague-Dawley rats were randomly divided into 4 groups (n=6):① control group (inhaled 0.9% normal saline for 3 hours, twice every day);② enalapril self-control group 〔intragastrically administrated enalapril 0.5 mg/only〕;③ model group (stimulated with 4 mg/L acrolein for 3 hours, twice every day);④ enalapril interventive group 〔inhaled acrolein and intragastrically administrated enalapril 0.5 mg/〕.

健康雄性SD大鼠24只，随机分为4组，每组6只。A组：空白对照组；B组：依拉普利自身对照组，依拉普利0.5mg/灌胃；C组：模型组，大鼠吸入4mg/L丙烯醛，每次3h，每天两次；D组：依拉普利干预组（吸入丙烯醛＋依拉普利灌胃），处理同模型组，并给予依拉普利0.5mg/灌胃。21d后处死大鼠，采集标本。

Results In all the animals injected with TGF-β, condrogenesis and osteogenesis were found under the periosteum and both intramembranous and endochondral osteogenesis were observed.

将TGF-β纯品、TGF-β粗提品、BSA及PBS分别注射于小鼠股骨骨膜下，最多连续注射9天，分别于4，7，14和21天处死小鼠，观察注射局部的组织学变化。

After model reproducing, the rabbits were randomized to receive a directly intramyocardial injection of either pcDNA3-bFGF 100μg (n=19) or pcDNA3 100μg (n=18). Animals were sacrificed 2, 6 or 12 weeks later, immunohistochemistry analysis was performed to study expression of bFGF gene at protein level, histologic analysis was performed to evaluate the effect of angiogenesis induced by gene therapy.

模型制备成功后将动物分为治疗组(n=19)和对照组(n=18)，并于心肌内分别注射pcDNA3-bFGF100μg或pcDNA3 100μg，饲养至2、6、12周处死；免疫组化观察蛋白表达；行病理切片观察缺血坏死心肌面积的变化和缺血心肌内血管新生的情况。

Methods: The experimental silicosis model in rats was established by intratracheal instillation of silica.

建立大鼠矽肺模型，分别在染尘后第1、8、15、22、29、36、43、50、57和64d各处死3只试验组和1只对照组大鼠。

On the 2nd, 4th and 6th day after intratracheal instillation, 1/3 rats in each group were killed to determine the concentration of IFN-γ, IL-6 and TNF-α in blood.

分别与接种后的第2、4、6d分批处死动物，动态观察动物的干扰素－γ、白细胞介素－6(IL-6)、肿瘤坏死因子－α的变化情况。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。