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Tis true, madame," answered he,"that my father was a Girondin, but he was not among the number of those who voted for the king's death; he was an equal sufferer with yourself during the Reign of Terror, and had well-nigh lost his head on the same scaffold on which your father perished."

维尔福的脸涨的通红,&不错,夫人,&他回答道,&我的父亲是一个吉伦特党党员,但他并没有去投票赞成处死国王。在恐怖时期,他也和您一样是一个受难者,也几乎和您的父亲一样在同一个断头台上被杀。&

Tis true, madame," answered he,"that my father was a Girondin, 8ttt8.com he was not among the number of those who voted for the king's death; he was an equal sufferer with yourself during the Reign of Terror, had well-nigh lost his head on the same scaffold on which your father perished."

维尔福的脸涨的通红,&不错,夫人,&他回答道,&我的父亲是一个吉伦特党党员,但他并没有去投票赞成处死国王。在恐怖时期,他也和您一样是一个受难者,也几乎和您的父亲一样在同一个断头台上被杀。&

The neck of femur were cut and stined with Masson Golder Trichrome for bone histomorphomemetric contrast group analysis.

在给药后3、4.5、6个月每组各取10只大鼠进行检测,先测量体重,空气栓塞处死,分离股骨颈,获得股骨颈样本,经切片等处理后,显微镜下作骨形态计量学检测。

Methods]ninety femal sd rats,weight 230~280 gram,were rangdonmized into three groups: qiangguyin group,miacalcic group and control group.all rats were variectomized.after 10 weeks,the osteoporosis model succeed,the treatment group,contrast group were given qiangguyin,miacalcic respectively.the treatment group used qiangguyin everyday,1ml/g.the contrast group used miacalcic 0.72u/kg,everyday hyodermic.and 3,4.5,6 months after the treatment,kill 10 rats to explore.the neck of femur were cut and stined with masson golder trichrome for bone histomorphomemetric contrast group analysis.

方法]健康雌性sd大鼠90只,体重230~280g。随机分为3组,分别为治疗组、对照组和空白组,每组30只。分别采取切除双侧卵巢方法进行骨质疏松造模,10周造模成功后,开始给药,治疗组用自拟强骨饮灌胃,每日一次,每次1ml/g,对照组用密盖息,皮下注射,每日1次,每次0.72u/kg。空白组,不做处理。在给药后3、4.5、6个月每组各取10只大鼠进行检测,先测量体重,空气栓塞处死,分离股骨颈,获得股骨颈样本,经切片等处理后,显微镜下作骨形态计量学检测。

We compare it with the Votalin treating that have been proved effective on clinic during the experiment, and detect intraosseous pressure in the upper part of tibia and the content of nitrogen monoxidum in articular cartilages、 synovial membranes、 synovias of knee joint, we can initially identify the mechanism of action that this theropy treat on gonarthritis, supply the theoretical and experimental evidence for making general use of it on clinic.

以致骨内高压的方式造模,于兔腹股沟中点以下10mm处结扎并切断右侧股静脉建立兔膝骨关节炎模型,造模10周后各组采用相应的方法进行治疗,4周后将骨髓活检穿刺针穿刺达胫骨上端髓腔内,以三通管与传感器相连检测骨内压,后将动物处死,取膝关节软骨、滑膜、关节液,检测一氧化氮含量。

Degradation test in vitro was carried out in phosphate buffer solution (0.1M, pH7. 4) at 37 ℃. The buffer solution was changed daily. Degradation test in vivo was implanted the sample to subdermal in adult ICH rat in the scapular area lateral to the dorsal midline. At suitable time the samples were recovered. Molecular weight changes in surface layer and bulk of polymer sample were measured by GPC and weight loss was determined gravimetrically. It was found that the degradation behavior can be regulated by changing the composition of copolymers. The critical compositions from surface to bulk degradation behavior for PGCA, PLCA, PLMCA, PLDCA systems were 15-20, 20-30, 30- 40, 40 of mol percent GA or LA unit in copolymers, respectively. The degradation behavior of PGCA, PLCA, PLMCA, PLDCA systems were compared and analysed. Some factors influencing the degradation character, such as copolymer composition, hydrophobicity, crystallinity and enzyme affect etc. played important role.

体外实验中材料降解环境为37℃,0.1M,pH7.4磷酸缓冲溶液,每天换液,定期取样;体内实验中将聚合物试样埋置于ICH小白鼠背部肩胛骨皮下部位,定期处死小鼠,取样,将体内体外样品进行重量损失及试样内外层分子量变化测定,分析各聚合物试样降解行为特性,实验结果证明,改变共聚物组成,可以调节各聚合体系降解行为特性,对PGCA,PLCA,PLMCA,PLDCA共聚体系,交酯摩尔百分含量15-20%,20-30%,30-40%,40%分别为各体系内降解行为特性由表面降解型向本体降解型过渡的临界转折点,交酯含量较低的聚合物不同程度地表现表面降解行为特性,论文对各共聚体系体内外降解行为作了分析对照,例如共聚物组成对材料降解速度与降解行为的影响;生物体内酶对降解行为的影响;材料亲疏水性,聚合物链段结晶行为及碳酸酯结构对材料降解行为的影响等,得出交酯/环碳酸酯共聚体系降解行为一些共性和规律。

BEHEADED posthumously, as punishment for his part in the execution of Charles I in 1649, Oliver Cromwell's fate after death matches his grippingly controversial life.

由于在1649年参与处死查理一世,克伦威尔死后被枭首。

Study Design. After undergoing L5 hemilaminectomy, chromic gut suture was placed onto the DRG and the animals were sacrificed at various time-points.

研究设计:L5半椎板切除术后,置铬制肠线于背根神经节,实验动物在不同时间点处死

Samples were extracted 6 and 12 weeks after operation for histological, histochemical and immunohistochemical analysis.

术后 6周和 12周分别处死动物、取材,进行缺损区大体、组织学、组织化学和免疫组织化学分析。

The gene expression was evaluated at 3 days after injection by histochemical analysis.

术后3天处死动物,组织化学分析LacZ基因在心脏中的表达。

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