处死

Before the experiment and in the second,fourth week of the experiment,blood was taken from mice's epicanthic intravenous of each group to determine biochemistry index of SCr,BUN,blood potassium,blood sodium and radioimmunity index of PCⅢ,HA,LA.

在实验前及实验的第2、4周，分别从各组大鼠眼内眦静脉采血，测定血肌酐、血尿素氮、血钾、血钠等生化指标以及Ⅲ型前胶原、血清透明质酸、层粘连蛋白等放免指标，42d时从心脏直接采血复查上述各指标，而后处死动物取肾脏进行组织形态学及特殊染色检查。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule.

取健康SD大鼠60只，正常对照组和正常银杏叶治疗组各6只，其余48只采用烧烙法，烙闭大鼠左眼2条浅层巩膜静脉，制作大鼠持续性高眼压模型，从中选出眼压稳定在实验要求水平的大鼠30只，随机分为生理盐水组，治疗A组（每日EGb 50mg/kg）、治疗B 组（每日EGb 100mg/kg）、治疗C 组（每日EGb 150mg/kg）、治疗D 组（每日EGb 200mg/kg），治疗时间为1mo，处死大鼠后做视网膜全层铺片，对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. Then 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只，正常对照组和正常银杏叶治疗组各6只，其余48只采用烧烙法，烙闭大鼠左眼2条浅层巩膜静脉，制作大鼠持续性高眼压模型，从中选出眼压稳定在实验要求水平的大鼠30只，随机分为生理盐水组，治疗A组（每日EGb 50mg/kg）、治疗B 组（每日EGb 100mg/kg）、治疗C 组（每日EGb 150mg/kg）、治疗D 组（每日EGb 200mg/kg），治疗时间为1mo，处死大鼠后做视网膜全层铺片，对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只，正常对照组和正常银杏叶治疗组各6只，其余48只采用烧烙法，烙闭大鼠左眼2条浅层巩膜静脉，制作大鼠持续性高眼压模型，从中选出眼压稳定在实验要求水平的大鼠30只，随机分为生理盐水组，治疗A组（每日EGb 50mg/kg）、治疗B 组（每日EGb 100mg/kg）、治疗C 组（每日EGb 150mg/kg）、治疗D 组（每日EGb 200mg/kg），治疗时间为1mo，处死大鼠后做视网膜全层铺片，对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只，正常对照组和正常银杏叶组各6只，其余48只采用烧烙法，烙闭大鼠左眼2条浅层巩膜静脉，制作大鼠持续性高眼压模型，从中选出眼压稳定在实验要求水平的大鼠30只，随机分为生理盐水组，治疗A组（每日EGb 50mg/kg）、治疗B 组（每日EGb 100mg/kg）、治疗C 组（每日EGb 150mg/kg）、治疗D 组（每日EGb 200mg/kg），治疗时间为1mo，处死大鼠后做视网膜全层铺片，对RGCL神经元做特异性染色后行神经元计数来评估神经元的情况。

Methods The chronic ocular hypertention rat model was made by cauterizating three episcleral veins.Rats were divided into control group,high level PROG group,middle level PROG group,low level PROG group according to different concentrations of PROG injected intraperitoneally.The left eye was model eye and the right eye was control eye.Three months later,the animals were executed and the eyeballs were enucleated.The RGCs were detected by HE staining and Thy-1.1 immunohistological staining.

通过烧灼3条巩膜上静脉制作慢性高眼压动物模型，此模型按腹腔注射不同浓度的孕酮而分为高、中、低浓度孕酮注射组及空白对照组，左眼为模型眼，右眼为自身对照眼，3个月后处死动物，取眼球制石蜡切片，行HE染色、Thy-1.1免疫组化染色及TUNEL原位凋亡检测。

That's how they were executed.

这就是她们被处死的原因。

When 60% of the spinal cord was compressed in two months, rat gastrocnemius muscle was acquired in ...

慢性压迫组处死大鼠后取腓肠肌作为标本；减压组彻底减压后分别于 5 ，10 ，15 ，2 0 ，2 5及 30d取材。

The neurological deficits and the sizes of cerebral infarction were measured. The contents of MDA and SOD in brains were also tested. Results The neurological deficits and the sizes of cerebral infarction decreased in 6 gingerol treated groups.

缺血组，6 姜酚小剂量组(2mg/kg)，6 姜酚大剂量组(4mg/kg)，尼莫地平组，缺血前30min舌下静脉给药，持续性缺血6h后处死动物，测定大鼠局灶性脑缺血神经系统症状，脑梗死体积，脑组织MDA和SOD含量的表达。

"Tis true, madame," answered he,"that my father was a Girondin, but he was not among the number of those who voted for the kings death; he was an equal sufferer with yourself during the Reign of Terror, and had well-nigh lost his head on the same scaffold on which your father perished."

维尔福的脸涨的通红，&不错，夫人，&他回答道，&我的父亲是一个吉伦特党党员，但他并没有去投票赞成处死国王。在恐怖时期，他也和您一样是一个受难者，也几乎和您的父亲一样在同一个断头台上被杀。&

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。