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At day 3, 6, 9, 14, 28, 42 and 56, five rabbits were selected from every group randomly and were killed by aeroembolism respectively. Their radius were taken out and the left one was taken as the research object. Immunohistochemistry stain and in situ hybridization stain were performed to examinate the VEGF and VEGF mRNA expression in the haematoma, fibrous callus and soft callus.

分别于术后3,6,9,14,28,42,56 d每组随机处死5只家兔,取左侧桡骨标本作为观察对象,通过免疫组化和原位杂交方法检查骨折端血肿、纤维骨痂和软骨骨痂中VEGF及VEGF mRNA表达。

Sixteenth week after operation, the animals were sacrificed by aeroembolism. Cartilage grading was observed by general observation and operation microscope. Specimens were procured from the weight-bearing portion of the femoral condylar, and evaluated according to a modified histological-histochemical grading system (0-1 point as normal and over 10 points as severe) by haematoxylin-eosin and Safranin'O/Fast Green stainings.

术后第,6周空气栓塞法处死动物,大体观察及手术显微镜下观察软骨表面分级;取股骨髁负重面软骨标本进行苏木精-伊红、沙黄染色及骨关节炎组织病理学评分(正常为0~1,重度为10分以上)、分级。

RESULTS: The defects of articular cartilage were repaired with osteofibrous tissue in group C and B.

术后1、2、4、8、12周处死实验动物,D组动物和手术组于术后1周和12周同时处死

Animals in those two groups were sacrificed at 24-hour, 72-hour and 7-day after model establishment, six were killed in each group at each time. The tissue of brain was taken out as soon as possible, and the activity of SOD was assayed with auto-oxide of pyrogallic acid.

两组动物分别于造模后24 h,72 h,7 d处死,每次每组处死6只,迅速断头取脑组织,采用邻苯三酚自氧化物测量SOD的活性。

The bronchial asthmatic model was established by egg protein sensibilization and longterm inhalation provocation.The rats of each treatment groups were lavaged each day from the first time of provocation to execution.After 4 weeks of treatment,the rats were killed to obtain lung tissue for dyeing of HE.A computer assisted image analysis system was used to determine the thickness change of bronchus wall and smooth muscle.The contents of MMP9 and TIMP1 in lung tissue were determined by ELISA double antibody sandwich method.

除正常组外以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型,各治疗组均从第1次哮喘激发开始(造模第3周)至处死前每天灌胃给药,激发并给药4 w后处死大鼠,取肺组织HE染色,彩色图像分析仪测量支气管管壁厚度、平滑肌厚度,采用ELISA双抗体夹心法测定肺组织MMP9、TIMP1含量。

On the way, he rises in the night, unseals their murderous commission, rewrites it and seals it with his father's ring, having worded it so that they themselves shall be the victims when they reach England.

在遣送去英国的路上,哈姆雷特私自拆开波罗尼斯给英国国王的信,发现波罗尼斯要英国国王等哈姆雷特到了英国后就处死他。于是哈姆雷特改写了那封信,并且用父亲给的封印盖封,让英国国王处死那两个背叛自己的朋友。

Thirty-six rats ,weighing(250±10)g, were randomly divided into normal group, experimental group and control group.Orthodontical devices were put between the upper incisor teeth and dens molaris in both experimental group and control group.The corrective force was adjusted to 60g.Salvia miltiorrhiza combination was implanted to each rat in the experimental group every day.The animals of both experimental group and control group were killed at 7,14 and 21 days.The upper dens malarias and periodontal tissue slices were observed under the light microscope and transmission electro microscope,and the X-ray dental films were taken and measured with digitization dental scanning apparatus and its software.

以36只体重(250±10)g的雄性大鼠为样本,随机分为正常组、实验组和对照组,设定不同浓度丹参复合膜,以同体对照方式在正常组进行体内植入实验,在2周内观察不同浓度丹参的牙周组织诱导作用,寻找最佳应用浓度;在实验组和对照组的上切牙与磨牙之间隙安装正畸装置,建立大鼠磨牙移动实验模型,矫治力的大小为60g;实验组每日每只体内植入丹参复合生物膜,两组动物于正畸加力7、14、21d,分三批处死处死后立即切取双侧上颌磨牙及牙周组织制取标本,制片透射电镜和光镜观察;拍摄X线牙片,用数字化牙科扫描仪及软件对其进行测量分析。

The corrective force was adjusted to 60g.Salvia miltiorrhiza combination was implanted to each rat in the experimental group every day.The animals of both experimental group and control group were killed at 7,14 and 21 days.The upper dens malarias and periodontal tissue slices were observed under the light microscope and transmission electro microscope,and the X-ray dental films were taken and measured with digitization dental scanning apparatus and its software.Result:The most fitting concentration of combined biological membrane was 0.80g;the speed of the remodeling of the periodontal tissue at the compression side in the experimental group was faster than that of the control group.

以36只体重(250±10)g的雄性大鼠为样本,随机分为正常组、实验组和对照组,设定不同浓度丹参复合膜,以同体对照方式在正常组进行体内植入实验,在2周内观察不同浓度丹参的牙周组织诱导作用,寻找最佳应用浓度;在实验组和对照组的上切牙与磨牙之间隙安装正畸装置,建立大鼠磨牙移动实验模型,矫治力的大小为60g;实验组每日每只体内植入丹参复合生物膜,两组动物于正畸加力7、14、21d,分三批处死处死后立即切取双侧上颌磨牙及牙周组织制取标本,制片透射电镜和光镜观察;拍摄X线牙片,用数字化牙科扫描仪及软件对其进行测量分析。

When I had consider'd this a little, it follow'd necessarily, that I was certainly in the Wrong in it, that these People were not Murtherers in the Sense that I had before condemn'd them, in my Thoughts; any more than those Christians were Murtherers, who often put to Death the Prisoners taken in Battle; or more frequently, upon many Occasions, put whole Troops of Men to the Sword, without giving Quarter, though they threw down their Arms and submitted.

于是,我对自己的计划也改变了看法,并开始冷静地考虑我自己的行动。我想:这么多世纪以来,上天都容许这些人不断互相残杀而不惩罚他们,那我有什么权力和责任擅自将他们判罪处死,代替上天执行对他们的判决呢?这些人对我又究竟犯了什么滔天大罪呢?我又有什么权力参与他们的自相残杀呢?我经常同自己进行辩论:&我怎么知道上帝对于这件公案是怎样判断的呢?毫无疑问,这些人并不知道他们互相吞食是犯罪行为;他们那样做并不违反他们的良心,因而他们也不会受到良心的谴责。他们并不知道食人是违背天理的罪行而故意去犯罪,就像我们大多数人犯罪时一样。他们并不认为杀死战俘是犯罪行为,正如我们并不认为杀牛是犯罪行为;他们也不认为吃人肉是犯罪行为,正如我们并不认为吃羊肉是犯罪行为。&我稍稍从这方面考虑了一下,就觉得自己不对了。我感到他们并不是我过去心目中所谴责的杀人犯。有些基督徒在战斗中常常把战俘处死,甚至在敌人已经丢下武器投降后,还把成队成队的敌人毫无人道地杀个精光。从这方面来看,那些土人与战斗中残杀俘虏的基督徒岂不一样!

The ethanol concentration in muscles, blood and vitreous body fluid of the rabbit which was drunk the ethanol and then killed after 2, 3 and 4 hours were respectively measured using head space gas chromatography. At the same time, the ethanol concentrations in muscles of the rabbit killed from 1 to 7 days were measured using the same method.

采用顶空气相色谱法分别测定了家兔灌服乙醇2、3、4小时后被处死后的肌肉、血液和玻璃体液的乙醇浓度,以及家兔被处死后放置1-7天的肌肉乙醇浓度。

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