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Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞,运用活细胞计数和碘化丙吮检测其增殖能力;采用流式细胞术检测其细胞表面标志的表达;用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化,并用Von Kossa染色进行鉴定;用地塞米松与胰岛素诱导其向脂肪细胞分化,并以油红O染色进行鉴定。

Results: The numbers of Bombesin positive pulmonary cells, the lamina propria S-l00 protein, neuron-specific enolase positive nerve fibers, IgE positive cells, mast cells and IgE positive mast cells significantly increased in bronchiectasis. The changes of pulmonary endocrine cells, nerve fibers and IgE positive cells were more significantly in hyperplastic BALT areas. The S-100 and NSE were found in lymphoid tissue and BALT. A close contact was found between mast cells and the S-100 positive nerve fibers. An IgE positive outer zone was found on MC surface. Mast cells and IgE positive cells were seen in the bronchial epithelium and alveolar septa.

结果:支气管扩张症中,支气管上皮蛙皮素阳性细胞、固有膜S-100蛋白和神经特异性烯醇化酶阳性神经纤维、IgE阳性细胞、MC和IgE阳性MC均显著增多,且在支气管相关淋巴组织增生的区域上述肺内分泌细胞、神经纤维和IgE阳性细胞增多尤为显著,S-100蛋白和NSE阳性神经纤维分布於弥散淋巴组织和BALT中,MC与S-100蛋白阳性神经纤维紧密接触,MC表面有IgE阳性环状带,MC和IgE阳性细胞出现在支气管上皮间和肺泡壁。

In the downstream, a sudden expansion microchannel with asymmetric pairs of microelectrodes is designed. The multi-electrodes are formed beside the expansion microchannel and result in the trap of micro-particles. AC electric potentials are applied to each pair of asymmetric electrodes creating a non-uniform electric field. The tangential component of the field produces a force on the induced charges in the diffused double layer.

此外在扩张管道上采用一种可调控式的微粒子撷取装置,主要是在扩张管道的侧边镀上多对的电极,利用电极间电场所引起的局部的电渗流(Electro-Osmotic Flow, EOF)和轴向压力驱动流彼此间相反的运动方向,进而在管道壁面电极区间产生回流区。

F, architectural style is to Cao Wei during the period of architectural styles on the basis, thus making a name / f,""汉风.contiouos the door of a large Brown Wall carving is members of the three Kingdoms period liubei and thought" Dongfang hero of the historical allusions," in the lobby of the right side is named " hero Pavilion " themselves mainly to providing customers with buffet breakfast, utencils, bars and other services, which could accommodate 60---90 people left and right; the left hand side is named " are the youyan tanghang isogenous sctudy diningcn," can accommodate more than 100 people at the same time, mainly to provide you with these dishes, Cantonese, peasant cuisine in a variety of cuisine, mainly as a zero.the first floor and procudtion 30per epidifferentiability between the different styles of gatherings, you can provide you with the wrong way of tea, catering, services, and 汉风/ f, there are various specifications of the Conference room, there are a number of accommodating more than 200 people of muiti, there are dozens of people can fit in, you can meet your different meetings and hospitality requirements.

汉风楼的建筑风格是以曹魏时期的建筑风格为基础,故起名&汉风楼&。迎门的大型古铜色的壁雕就是大家所熟知的三国时期刘备与曹操&青梅煮酒论英雄&的历史典故,大厅右侧是命名为&英雄阁&的西餐厅,主要为顾客提供自助早餐、西餐、酒吧等服务,可容纳60---90人左右;左手边是命名为&许都宴堂&的中餐厅,可容纳100多人同时就餐,主要为您提供湘菜、粤菜、农家菜等多种菜系,主要为散客提供零点服务。一楼和二楼有30余间风格不同的贵宾厅房,可以为您提供餐饮、茶艺、棋牌等服务,而且汉风楼还有各种规格的会议室多间,有同时容纳200多人的多功能厅,还有可以容纳几十人的中、小型会议室,可以满足您不同的会议及接待需求。

At interphase, there is no cytoplasmic channel in the cell walls of PMCs; At zygonema and pachynema, a lot of cytoplasmic channel, ranging in diameter from 150nm to 600nm, appears; After pachynema, the number of channels decrease rapidly.

在间期的花粉母细胞间未见胞质通道,胞质通道在偶线期前后形成并开放,在粗线期以后迅速减少。在细线期和偶线期,花粉母细胞细胞质膜与细胞壁之间的质外体出现大量的小泡,即所谓的壁旁体。

At last the paper realized the advantages and disadvantages of passive solar building and adnvanced some suggestions in future research. From another point, the paper logged the process of the building of the experimental equipment and the mounting of the measurement equipments that accumulated some operating experimence.Carrying experiments, calculating and analyzing the experimental data for more than 2 years, the paper gained some constructive conclusions. In winter the indoor air temperature and RH are lower than comfort criteria, but people feels very comfortable in the passive solar house. The thermo-circulation formed by Trombe wall play an important role in improving indoor air temperature and RH. Based on the analogy analysis theory, the natural convection heat transfer in the air gap can be treated as superposition of two natural convection heat transfer processes occurring over isolated isothermal vertical flat plate, in this case namely the massive wall and glazing. Two empirical formulas for Q and Nu are obtained. The investigation shows that the optimal time to open the damper should comply with the period that the thermo-circulation happens which can be controlled by thermal response characteristics of Trombe wall surface temperature and air temperature in the air gap.

经过两年多的实测和计算,根据传热学、流体力学、统计学等学科的基础理论,对大量数据进行整理归类、分析讨论,主要得到如下结论:(1)冬季,含有特朗贝墙体的被动式太阳房室内热湿环境较舒适性标准偏低,但人体感觉比较舒适,特朗贝墙体形成的热循环通风在调节室内温度和相对湿度方面起到很大的作用;(2)特朗贝墙体空气间层自然对流可以看作是两个等温独立大平板的自然对流换热过程的叠加,通过与大平板自然对流理论类比获得了计算热循环过程中对流换热量的经验公式;(3)特朗贝墙体通风口的启闭可以根据玻璃幕墙内壁面温度、蓄热墙体外壁面温度和空气间层进出口空气温度之间的关系来进行控制,从而有效地阻挡冬季冷循环的发生;(4)夏季,如没有有效的通风降温措施,被动式太阳房内会产生明显的过热。

Results CT and HRCT had more sensitivity and specificity than X-ray chest film.There were more image manifestations in HRCT such as grand grass,net,line,nodular,honeycombing,bronchiectasis,honeycombing cyst,peribronchovascular interstitial thickening and irregularity.There were significant difference between them (P.05).These patients who were suspected IIP had better control and remission after being treated with hormone and immuno-suppressive drug.

结果 胸部CT在诊断方面比常规胸片有较大的优越性,HRCT则有更高的敏感性和特异性,HRCT表现出磨玻璃影、网状、线条、小结节、蜂窝影,支气管扩张、细支气管扩张、支气管壁和血管壁增厚及不规则等征象,12例临床疑诊为间质性肺炎的患者,经胸部HRCT诊断的12例,而胸部平片诊断的仅有2例,二者比较差异有显著性(P.05),HRCT疑诊的IIP患者,经临床激素及免疫抑制剂治疗,病情均得到控制或缓解。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞,CM联合20%FBS诱导骨髓间充质干细胞,同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组,分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化,并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化,用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

Culms ca. 8 m, ca. 2.5 cm in diam.; internodes dull purple-green, becoming green with lower internodes tinged purple, to 35 cm, initially white powdery; wall ca. 5 mm thick; nodal ridge slightly elevated, as prominent as sheath scar; intranode ca. 5 mm. Culm sheaths pale red-brown, sometimes tinged green, sparsely to densely speckled, dark brown and glabrous along margins, sparsely strigose; auricles and oral setae well developed, purple; ligule purple, truncate to arcuate, short but broad, margin fringed with purple-tinged cilia; blade reflexed, purple to green, crinkled.

秆约8米,约厘米直径;节间愚笨紫色绿色,变得绿色与低节间紫色地微染着,令35厘米的是,最初白色粉状;壁约5毫米厚;节的稍隆起脊,象鞘痕一样突出; intranode约5毫米竿箨浅红棕色,有时微染绿色,疏生对浓密有斑点,暗褐色和沿边缘,疏生糙伏毛;叶耳和口头的刚毛发育良好,紫色;叶舌紫色,削去到弧曲,短但是辽阔,用紫色微染的cilia作为边缘的边;叶片反折,对绿色,皱曲紫色。

Along the winding mountain roads and on to the Peak, but see the deep green walls,diao liang hua dong ,hong zhu cornices, unique.qian dian the existing temple, the three permitted all three, the Temple Guardian Temple 1, Wan-dong and Shanmen, 2, Monk kilnsmu ta , Ling Yin ruins, such as three-ko.qian dian face south, the temple is located in the middle axis,dian qian Two monument erected the temple, built in the Qianlong sixteen years; Temple Guardian Templeqian dian house is located right in the middle of the permittedhou dian for the million, Inner Temple wall is complete, with high art, painting of the Heavenly Kings, like the Queen Mother, Monju, Fugen, as are 1.57 meters high, the proportion of moderate and natural, beautiful color and good facilities, rather than fine lines tangled complex, rigorous and lively layout;"Tang learn" vividly comic, realistic characters, new colors, easily sketched, are up-hing sent far-reaching effect of memorable art.

沿山路蜿蜒而上至山顶,但见红墙碧树深处,雕梁画栋,飞檐红柱,别具特色。寺院现存前殿、三佛殿各三间,韦驮殿一间,万宝洞及山门、二门、僧窑、墓塔、灵寅三层阁等遗迹。前殿面南,位于寺院中轴线正中,殿前竖庙碑二通,建于乾隆十六年;韦驮殿位于前殿屋后正中,后殿为万佛殿,内殿壁面十分完整,具有较高的艺术性,彩绘的天王像、王母像、文殊、普贤像均高1.57米,比例适中而自然,施彩艳丽而不俗,线条繁细而不紊,布局严谨而生动;&唐僧取经&连环画生动形象,人物逼真,色彩新颖,勾勒自如,均达到兴寄深远,回味无穷的艺术效果。

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Plunder melds and run with this jewel!

掠夺melds和运行与此宝石!

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻,也许正是在通往天国的路上,我体验着这白色的晕旋。

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单击"保存"会将文件保存到主持人的硬盘或服务器上,而不是您自己的计算机上。