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Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法:穿刺抽取15 mL脐带血,密度梯度法分离吸取分层界面的白色环形絮状物,其中富含单个核细胞,离心后沉积细胞用含15%胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮,调整细胞密度为1×109 L-1接种于25 T培养瓶内,放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养,72 h后更换培养基,去除红细胞及其他未贴壁细胞,至70%~80%融合时传代。

Do not overfill boxes of food, food, and between the walls of the box Go out for at least 1 cm gap.

箱内食物不要过满,食物和箱壁之间至少要留出1厘米的间隙。

Methods:Cardiac muscle of twoday neonate rattus was digested repeatedly by 0.0625% trypsin.The cardiocytes were collected in DMEM medium,which contains 10% fetal bovine serum.Myocardial cells were isolated with the technique of differential anchoring velocity with 90 min and were pured by Brdu.The cardiocytes were placed into CO2 incubator to incubate seven days after their purification.

应用0.0625%的胰蛋白酶重复消化出生第2 d乳鼠的心肌组织多次,收集的细胞用含10%胎牛血清的DMEM培养基中和,用差速贴壁分离法分离,在以溴脱氧尿嘧啶纯化心肌细胞后置CO2培养箱孵育7 d。

The storage tubes (22) into which the tubes (23) telescopically stored from two wide of a four-sided framed extending along the entire walls for the rail of the case to provide structure for the case without interfering with the packing items.

一存储套管(22)沿着该行李箱的围挡部分的整个箱壁延伸,以为行李箱提供不影响装载物品的结构,其中该伸缩套管(23)从一四侧面框架的两个较宽侧存储在该存储套管(22)中。

The box beam external dimensions are big , are away from the ground to be high , carries on the dog-legged single shade beam crack detection from the opposite side , the focal distance is long , and is not easy to operate.

箱形梁外形尺寸较大,距离地面较高,从对侧进行双壁单影射线探伤,焦距偏长且不易操作。

Item 1 22000 Double-wall corrugated fibreboard box.

项目1 2.2万双壁波纹纤维板箱。

Stem packing are generally Check Valves close to the cold box in the stem wall of the filler tank.

阀杆填料一般都在阀杆紧贴冷箱壁处的填料槽内。

Studies the influents of aluminum foil,as well as the contact length on the heat transfer.

因此,对冷凝管与箱壁平板的线接触进行了改进,采用凹槽结构的接触形式,并分析接触角度对散热的影响。

A penetration through the box wall greater than 0.50 inch is not acceptable.

C。 渗透箱壁直径大于0.50英寸,不接受每一纸箱,不得超过一处

A penetration through the box wall greater than 0.50 inch diameter is not acceptable.

C。 渗透箱壁直径大于0.50英寸,不接受每一纸箱,不得超过一处

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随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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