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Methods Human umbilical cord blood mononuclear cells were isolated by Ficoll density-gradient centrifugation. EGM-2 culture fluid was added, and then cells were plated on dishes coated with human fibronectin. After 48 h, the nonadherent cells were collected and replated on fibronectin-coated dishes.

采用Ficoll密度梯度离心法从人脐血中分离单个核细胞,加入EGM-2培养液,接种于包被人纤连蛋白的培养皿中贴壁培养,收集48h后的悬浮细胞重新贴壁培养至第7天,利用免疫荧光和流式细胞术鉴定。

METHODS: Bone marrow mononuclear cells were isolated from 5M4/M5 leukemia patients with high CD14 expression, and then divided into 3groups: adherent leukemia cells, nonadherent blasts, and total unfractioned blasts.

取5例初诊CD14高表达的M4或M5型白血病患者的骨髓标本,分离单个核细胞(bone marrow mononuclearcells,BMMNCs),将白血病细胞分为3组:贴壁白血病细胞组、非贴壁白血病细胞组及总白血病细胞组。

CT appearance of paragonimiasis of the lung is similar to other inflammatory diseases,and the differentiation sh...

肺吸虫病的胸部CT表现与一般感染性病变不易区别,应结合临床相关检查,但是附壁结节空洞内的&瓜子&状附壁结节影有助于诊断。

Methods 14 teeth with iatrogenic root canal perforation were selected. The root canals were shaped and cleaned, and then obturated with gutta- percha after MTA was used to repair the perforation in the condition with any blood and moisture.

选取医源性因素造成的根管侧壁穿孔患牙14颗,彻底清理根管并预备根管,在止血隔湿的条件下以MTA 即刻修补根管侧壁穿孔。

Using of agarose-embedment lysis cell wallmethod for preparing intact chromosomal DNA from phytopathogenic fungi is thefirst time.

首次采用凝胶包理破壁法由植物病原真菌分生孢子获得完整染色体DNA,克服了先用裂解酶破壁获得大量原生质体再用琼脂糖包理原生质体的传统制备方法的一些不足。

By non-enhancement scan, 12 cases showed low density mass, 4 cases equal density mass, 1 case slightly high density mass, and 2 cases stippled calcification mass. By enhancement scan, 13 cases showed inhomogenous enhancement in cortical phase, 2 cases showed slight to moderate enhancement. Another 2 cases of small cystic RCC presented enhancement of wall with nodules and the partition. The enhancement of all tumor cases faded away in the following parenchymal phase and pyelographic phase, demonstrating some degrees of "fast washing out" phenomenon; 4 cases presented with pseudocapsule. The reconstruction of 17 "MPR/MIP" and 9 "VR" showed clearly the relations of kidney vessels, tumors and neighbor tissues and accessory renal arteries were founded in 2 cases.

结果:17例中小实性肾癌15例,小囊性肾癌2例;CT平扫呈低密度12例,等密度4例,稍高密度1例,伴有斑点状钙化2例;增强扫描皮质期13例呈不均匀明显强化,2例呈轻中度强化,另2例小囊性肾癌的囊壁、壁结节及囊内分隔明显强化;实质期、肾盂期扫描所有肿瘤强化均迅速减退,呈&快进快退&改变;4例可见假包膜。17例MPR/MIP、9例VR重建均能清楚显示肾血管、肿瘤与邻近组织的关系,并发现副肾动脉2例。

Injection of dehydrated alcohol into the retropubic space induced chemical inflammation adhesion of vaginal wall with pubis at puncture on suppubis into vagina for suspension of vaginal wall and neck of bladder and urether.

方法耻骨后间隙注射无水酒精并经耻骨上穿刺,利用阴道托将阴道前壁连同膀胱颈及尿道托向耻骨,无水酒精引起化学性炎性粘连起到固定的作用,阴道前壁起到类似无张力吊带的作用。

Methods:30 nomal subjects and 12 old myocardial in farction patients were inclade. The wall motion scove were analysed by experieced obsservers and less experienced obsevers and ck techniques.

分别对30例健康者及12例经冠脉造影证实为左冠状动脉狭窄≥75%的陈旧性心肌梗塞患者的左室各节段室壁运动状态用常规室壁运动分析法和CK技术进行超声评分。

Through analyzing the problem of the coal wall support, top-coal caving and the design of powered support,the author explains the method to control the coal slabbing and the measure of powered support design and the collocation of the rear transport pan.

通过对仰采放顶煤工作面煤壁支护、放顶煤问题、液压支架设计等方面的分析,说明在仰采工况下控制煤壁片帮方法以及液压支架设计、后部输送机布置应采取的措施。

Methods Vascular specimens from 41 patients with thromboangiitis obliterans and 14 normal controls were analyzed by PCR. The nucleic acid sequence of enhancer located in the upstream of immediate early gene of cytomegalovirus was used as target region for detection of cytomegalovirus DNA.

方法以人巨细胞病毒即刻早期基因上游的增强子为扩增区段,用PCR技术分析了41例血栓闭塞性脉管炎病变血管壁中和14例正常血管壁中人巨细胞病毒的感染情况。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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