增殖的

The mechanism of PPS accelerating IEC-6 cells migration was also researched with gene-chip technique. The Altas Clontech Rat cDNA Chip which consisted of 1176 spots representing different functional categories such as heat shock/stress, cell cycle, transcription factors, hormones, metabolism, etc was used. Result showed in cell migration model of IEC-6 cell line, compared with control group, expression of 38 genes in total 1176 genes varied in cells treated by PPS, including 21 genes up-regulated and 17 genes down-regulated. Date analyze showed the mechanism of PPS accelerating IEC-6 cells migration mainly related with promoting the numbers and activity of cellular ion channel, especially 〓 channel.

从以上实验结果中我们可以得出以下结论：四君子汤总多糖具有促进IEC-6细胞增殖、迁移、粘附的作用；四君子汤总多糖无论是对IEC-6细胞增殖、或者迁移的作用均优于各单味药多糖，可能是各种单味药多糖综合作用的结果；多糖可能是四君子汤"益气健脾"药效的主要成分；小肠上皮细胞可能是四君子汤总多糖的作用靶点之一，提示临床采用四君子汤及"益气健脾"法治疗"脾虚证"的疗效机制可能与增强机体的整体免疫和肠道粘膜免疫功能有关。

The peroxisome proliferator-activated receptors play an important role in adipocyte differentiation and fatty acid metabolism, activation of PPAR may induce the differentiation of adipocyte,influence the expression of genes involved in lipogenesis and fatty acid oxidation,eventually lessen ectopic fat accumulation and improve the impaired insulin signaling,so the medicine which targets to the control point of FFA metabolism would be hopeful in the treatment of a cluster of metabolic abnormalities resulted from insulin resistance.

过氧化物酶体增殖物激活受体在脂肪细胞分化、脂肪酸代谢中起重要作用，PPAR及其所调控基因的激活可促进脂肪细胞分化、减少脂质产生、增加脂肪酸氧化，从而减少脂质的异位沉积，进一步改善损伤的胰岛素信号转导通路，逆转胰岛素抵抗。针对脂肪酸合成及氧化的关键调控点的药物可能是今后治疗胰岛素抵抗引发的一系列代谢异常的新的靶点。关键词过氧化物酶体增殖物激活受体；脂肪酸代谢；胰岛素抵抗

The degradating and redistributing of ECM were migration of VSMC and vascular remodeling through inhibiting the production of PDGF-BB and the activation of MMPs so to prevent the vascular stenosis.⑤Orthotopic hybridism in situ: The expression of MMP-3 increased after operation, the tendency and intensity were parallel to the expression of NF-κB. Tongxinluo could decrease the expression of MMP-3 and NF-κB. The study showed that the producting and activating of MMP-3 were modulated by NF-κB. The inhibition to the producting and activating of MMp-3 of Tongxinluo was mediated by inhibiting the activating of NF-κB. Conclusion ①The rabbit vascular stenosis model could be established successfully by plain balloon damage, the operation process was easy, economical and practical.

①单纯球囊损伤可成功建立典型家兔血管狭窄模型，操作方法简单，经济实用；②以VSMC增殖、迁移为主的内膜增厚以及以ECM降解、合成与再分布所致的血管重构是导致受损血管狭窄的根本原因；③通心络能明显抑制VSMC的增殖、迁移和ECM降解、合成，阻止血管内膜增生和血管重构，防止受损血管狭窄；该作用可能与其减少和阻止PDGF-BB的产生和活性，以及抑制MMPs的表达和恢复MMPs/TIMPs平衡有关；④通心络能显著抑制球囊损伤后血管内膜增生和血管重构，可能与其保护血管内皮，改善内皮功能，增加血清NO含量有关；⑤通心络抑制内膜增生和血管重构，减轻受损血管狭窄，还可能是通过抑制NF-κB的活化途径而起作用的。

Results:Whith the time and doses of ART incubation extended, ART significantly inhibited the proliferation of SGC-7901 cells and the inhibited effect shows dose-and -time-dependent. Obvious changes of apoptotic morphology were observed by invert microscope, fluorescence microscope, scanning electron microscope and transmission electron microscope, they showed that cells had marked nuclear condensation or the fragmentation of chromation as well as apoptotic , mitochondrial edema and vesicle , with the time of incubation extended , the proliferation rate become slow and the volume became small and transformed. FCM assay indicated that most of the cells were arrested in Go/Gi and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. At the same time, the number of S and G2/M phase cells were decreased. The result of TUNEL indicant that there are apoptosis and necrosis.

结果：随着药物浓度的增加和作用时间的延长，蒿甲醚对胃癌SGC-7901细胞的抑制作用呈时间和浓度依赖关系：在倒置显微镜、荧光显微镜、扫描电镜和透射电镜下可观察到典型的凋亡细胞的形态改变，表现为：核固缩或染色质边聚或凝聚成大块状，可见凋亡小体，线粒体肿胀增殖，严重的空泡化，随作用时间的延长，细胞增殖速度减慢，细胞体积缩小变形；流式细胞术显示胃癌SGC-7901细胞出现明显的凋亡峰，随着作用时间的延长，其凋亡率逐渐升高，细胞周期阻滞在G_0/G_1期，S及G_2/M期细胞数大量减少；流式细胞术TUNEL检测结果显示细胞凋亡和坏死同时存在。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

To investigate the stantant changes of Ⅰ、Ⅱ、Ⅲ and PG expression of mandibular condylar chondrocytes stimulated by the different static compressive stresses, so that to make a primary understanding of the relationship between the differentiation and dedifferentiation of condylar chondrocytes and different compressive stresses; to investigate the stantant changes of the content of DNA of mandibular condylar chondrocytes stimulated by the different static compressive stresses, so that to make a primary understanding of the relationship between proliferentiation and apothesis of MCCs and different static compressive stresses.

观察单层培养的髁状突软骨细胞加载不同静压力后，Ⅰ、Ⅱ、Ⅲ型胶原以及蛋白多糖表达的变化，探讨不同静压力对髁状突软骨细胞的分化及去分化的即时效应；观察单层培养的髁状突软骨细胞加载不同静压力后，其增殖、凋亡及细胞周期的变化，探讨不同静压力对髁状突软骨细胞增殖及凋亡的即时效应。

The human cartilages are composed of chondrocyte and extracellular matrix , the form of chondrocytes are hypertrophy and the quantity are less; the ECM of cartilage are compised of type Ⅱ collagen and proteoglycan. Articular cartilages are all hyaline with little fibers. Trauma and arthritis are the main cause of cartilage injury, the ommilayer injury ofcartilage can be recovered by marrow, but because of without stimulation mechanism, the new tissues are merely fibrocartilages, they can not be coincide with hyaline cartilage in menchanics; the purely damage of articular cartilage can not stimulate chondrocyte to regenerate because of without blood circulation, thus, the plerosis of articular catilage can not depend on the proliferation of local chondrocyte.

造成人体关节软骨损伤的原因主要为创伤和关节炎，关节软骨全层损伤可由于骨髓中间充质干细胞的高速增殖修复，但这种修复由于缺乏相应的刺激机制，只能形成纤维软骨，而不能形成符合关节生理、力学要求的透明软骨；单纯软骨部分损伤软骨组织内无血管，软骨细胞迁移迟缓，无法使损伤区域软骨细胞再生，因此，关节炎及关节创伤后的软骨修复不能依赖于软骨细胞的增殖和迁移。

The increment of 6-BA concentration can increase the protocorm propagation obviously. And the matching of high concentration of 6-BA and low concentration of NAA is more suitable for the protocorm propagation of C. grandiflorium. 6-BA at the concentration of 1.5 mg/L was most favorable for protocorm induction and differentiation.

结论］6-BA浓度的增加，可明显提高原球茎的增殖，高浓度6-BA与低浓度NAA的配比较适合大花蕙兰的原球茎增增殖；6-BA在1.5 mg/L时最有利于原球茎的诱导分化。

Among three auxins , IAA at low concentration was the best for growth of Type Ⅰ hairy root, while 1mg/l IBA was the most suitable for growth of Type Ⅱ hairy root.

在发状根的继代培养基中添加外源生长激素（IAA、IBA、NAA），低浓度的IAA更适合于Ⅰ型发状根的增殖培养，而在促进Ⅱ型发状根的增殖和侧根形成方面，1mg/lIBA的作用最强。

Comparing as BA, 0.1mg·L〓 TDZ was more effective in inducing germinating and developing of buds, regenerating prolific caespitose buds via organogenesis, promoting rhizogenesis and activity of root system greatly, as a result, acclimation survival rate was improved significantly. When leaf segments, cut from in vitro plantlets, were placed on MS media containing 0.1mg·L〓 TDZ, adventitious buds regenerated from segments. After transferred to propagated and rooted media, adventitious buds would proliferate and come into full plantlets.

与BA相比，TDZ的细胞分裂素活性至少高出一个数量级，0.1mg·L〓的TDZ能有效地诱导四个基因型芋茎尖的萌动、生长、大量增殖丛生芽，促进试管苗根系发生，将根系活力提高2倍以上，显著提高了试管苗的驯化成活率；浓度为0.01mg·L〓的TDZ能诱导魁芋类型的芋离体叶片切块产生再生芽点，转接后可增殖不定芽并形成再生植株。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。