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Objective: To investigate the effect of inhibitng C-myc expression by actinomycin D on intimal hy-perplasia in vein graft.

目的:观察放射菌素D对移植血管中C-myc基因表达及内膜增殖的影响。

Induction of HO-1 mRNA was inhibited by actinomycin D and was associated with inhibition of RASMC proliferation.

放线菌素D抑制HO-1 mRNA表达增加并影响RASMC增殖的抑制。

METHODS: MTT method and soft agar assay were performed to observe the effects of the resveratrol on the cell growth.

目的:观察虎仗提取物白藜芦醇对胃癌细胞HGC27细胞周期和增殖的影响及凋亡诱导作用。

Objective: To investigate the effect of aldosterone on the proliferation of cultured myocardiac fibroblasts in vitro.

目的:研究醛固酮对培养心肌成纤维细胞增殖的影响。

The effect of rhBMP-2 on differentiation and proliferation of the stem cells was studied by determination of ALP and MTT activity.

探讨体外条件下rhBMP-2诱导脂肪来源干细胞向成骨细胞分化和增殖的情况,并观察其量效关系。

The effect of ammonium chloride ( NH4Cl ) on the growth of micro algae Dunaliella viridis was reported.

报道了氯化铵对绿色杜氏藻增殖的影响情况。

DMBQ, ferulic acid and resorcin were effective chemical signal substances in the process of seed germination and haustorium formation of Cistanche deserticola. 4.The embryo is consist of 12 cells, the endosperm cell degraded so as to provide nutrition for the embryo development .As a result, germ tube differentiated from the embryo and came out through micropylar end, the small cell at the tip of the germ tube held the capacity of cleavage and the other cells grew big ,so the germ tube elongated .The anterior extremity of germ tube swelled to form haustorium under chemical substances treatment, the haustorium cell stained ,especially its portrait because of the lignification of the cells.

肉苁蓉种子的胚是由12个细胞紧密排列形成的圆球状胚,球形原胚分裂增殖的同时,胚乳细胞分解,为胚细胞的增殖提供营养,最终胚上分化出的芽管由珠孔端冒出,芽管前端细胞较小,具有旺盛的分裂能力,后部细胞体积增大,芽管逐渐伸长,在化学物质的诱导下,芽管由中部至前端细胞膨大分化出初生吸器,初生吸器呈帽子状,而这些细胞比其周围的细胞着色深,吸器纵向局部区域的番红染色加深,细胞高度木质化。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实:两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用,并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著,最佳效应浓度为400nM;2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响:①CREG蛋白对VSMC迁移的影响:刮伤实验发现,加入最佳效应浓度的wtCREG和mCREG蛋白24h后,OB2组迁移能力下降,HITASY组无明显变化;细胞外基质金属蛋白酶-2,9(Matrix metallo-proteinase 2,9,MMP2 ,9)明胶酶电泳检测和Western blot检测结果证实,两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2,9减少,而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases,TIMPs)增加;②CREG蛋白对VSMC分化的影响:加入400nM的wtCREG和mCREG蛋白12h后,OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加,LM-1、FN表达减少;③流式细胞仪分析细胞周期和BrDU染色分析证实,加入400nM的wtCREG和mCREG蛋白后,OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572,HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034;3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用:①免疫共沉淀和免疫荧光双染色分析结果显示,CREG蛋白与M6P/IGF2R存在直接结合;②应用抗体阻断实验:将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中,CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱,而且与加入anti- M6P/IGF2R浓度正相关。

The results indicated that:(1) 4 d or 6 d after denervation, increase of endocyto--sis and proliferation of satellite cells in denervated muscle were induced.(2) Actino--mycin D inhibited activation of satellite cells and endocytosis in normal muscle.

结果表明:(1)去神经4d或6d的肌肉可引起胞纳的增加和卫星细胞的增殖;(2)放线菌素D抑制正常肌肉的卫星细胞激活和胞纳作用;(3)在去神经的肌肉中,放线菌素D抑制了卫星细胞增殖的同时还抑制了胞纳的增加,但不能阻止去神经肌肉的萎缩。

Though the primary stimulator of 1H is not clear, it is related to physical, cell and body fluid IH is a chronic process involving multiple faction injury and blood flowing abnormal are revamped as the main reason, the proliferation level depend on the length and width of the injure of vessel large amount research illustrate injury cause many growth factor infolded in IH, for example: template derived growth factor, fibroblast growth factor, transmission growth factor , endothelin (ET-1) et al, some early responsive gene such as c-fos、c-jun c-myc also take part in SMC proliferation after vessel injury.

虽然IH的确切起始刺激因子并未阐明,但已知与物理、细胞和体液因子有关。IH是一个多因素参与的慢性过程,损伤和血液动力学异常一真被认为是IH的主要因素。内膜增殖的程度有赖于血管损伤的长度和深度。近年来大量的实验研究结果表明,损伤等刺激引起的众多的生长因子样物质在IH中起关键作用。如血小板源性生长因子、碱性纤维母细胞生长因子、转化生长因子β、内皮素-1(ET-1)等诸如C-fos、C-jun、C-myc等早期应答基因也参与了血管损伤后SMC的增殖。

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Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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