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Treatment of canine osteoarthritis with insulinlike growth factor1 (IGF1) and sodium pentosan polysulfate〔J〕.

IGF1是一种细胞增殖调控因子,在细胞的分化、增殖、个体的生长发育中具有重要的促进作用。

The sry-gene chimerism of bone marrows, spleen and thymus of the receiptor, T-lymphocyte subpopulation of peripheral blood cells, ConA and LPS induced T and B cells proliferation activity tests, Mixed lymphocyte reaction between the donor and the receiptor and the third part, the incidence rate of GVHD and survival analysis were observed in mice after 8Gy TBI with MSCs and haplo-BMT treatment together.

通过观察半相合BMT联合MSCs治疗致死剂量照射小鼠后不同时间骨髓、胸腺和脾脏中供者细胞植入,外周血中的淋巴细胞亚群,ConA和LPS刺激的T、B淋巴细胞增殖,供受者及第三者混合淋巴细胞反应,急、慢性GVHD的发生率和生存分析,提示MSCs促进骨髓干细胞植入,加快T淋巴细胞增殖,促进体液免疫恢复,通过供受者免疫耐受,减少GVHD发生率,提高半相合BMT动物生存率。

Methods The effects of various concentrations of tea polyphenols on proliferation, invasion and apoptosis of CGTH W-3 cells were observed by MTF assay, plate scarification assay and flow cytometer, respectively.

方法用MTT法检测细胞增殖活性,观察不同浓度茶多酚对CGTH W-3细胞增殖活性的影响;并通过培养板划痕法观察茶多酚对CGTH W-3细胞侵袭能力的影响;应用流式细胞仪检测各浓度茶多酚诱导CGTH W-3细胞凋亡的作用。

Result: Compared to those from corresponding normal tissues the expression of Tob mRNA in colorectal cancer tissues were significantly increased.

F j;^ Tob基因是抗增殖基因,可抑制细胞的增殖[1],最近的研究表明:在肺癌的发病机制中Tob可能为抑癌基因[2]。

Proliferation assay, cellular cycle and apoptosis analysis, invasion and migration assay and adherency assay showed that GEBP11 could inhibit the proliferation of Co-HUVECs and HUVECs, induce the apoptosis of ECs, but not alter the cell cycle of ECs.

MTT细胞增殖实验、细胞周期及凋亡分析、细胞侵袭迁移实验、细胞粘附实验显示,GEBP11短肽对Co-HUVECs及HUVECs的增殖表现出不同程度的抑制作用,对Co-HUVECs抑制作用更强,GEBP11能诱导内皮细胞的凋亡,而对细胞周期无明显影响。

EGF has the auxoaction on proliferation of the cultured fibroblasts in vitro. Collagen scaffold has some regulatory effects on proliferation rate of the cultured fibroblasts in vitro.

EGF对FB的增殖具有促进作用,而胶原网架对FB的增殖速率具有一定的调节作用。

By Percoll fluid and density gradient centrifugation,the MSC was obtained;after the cells filled the bottom of vessel,subcultured them,when they subculture in third generation ,redigested them,500 R/min centrifugate,alter the

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞,这种细胞在体外培养体系中保持旺盛的增殖能力;经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化,增殖旺盛,可传一代培养,是软骨组织工程理想的种子细胞。

MSC can be obtained by percoll fluid and density gradient centrifugation, this cells kept proliferation and differentiation ability in vitro; MSC can differentiate into chondroblast by lightly centrifugation and chemical definition medium, after one generation, the differential cells kept the phentype of chondroblast, they are a kind of ideal seed-cells for cartilage tissue engineering.

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞,这种细胞在体外培养体系中保持旺盛的增殖能力;经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化,增殖旺盛,可传一代培养,是软骨组织工程理想的种子细胞。

Part one Isolating, culturing, chondroblast induction and evaluation of human MSC in vitroObjective:To examine the property and proliferation ability of primary culturing MSC ,mvestgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

结果:原代骨髓基质干细胞易于体外培养,增殖旺盛;经轻度离心和化学限定培养基诱导后细胞呈高密度生长,仍保持较旺盛的增殖能力,细胞转化成圆形肥大细胞,甲苯胺蓝染色显示软骨细胞外基质 v的特异性异染,11胶原蛋白免疫细胞化学染色呈强阳性,BT干CR鉴定显示*胶原mRNA丰富的表达。

Objective To compare the growth and proliferative characteristic of cultured fibroblast cells taken from pterygial and normal conjunctival specimens.

目的 对比观察相同的培养条件下翼状胬肉与正常结膜成纤维细胞的生长增殖状况,对两种成纤维细胞的生长增殖特性作出评价。

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