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Effects of mechanical strain on proliferation and differentiation of Saos-2 osteoblastic cells Objective: To investigate the effects of mechanical strain on proliferation and differentiation of Saos-2 osteoblastic cells.

机械牵张应力对成骨细胞增殖和分化的影响目的:研究机械牵张应力对成骨细胞增殖和分化能力的影响。

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb,用Ang Ⅱ诱导促进其增殖,采用MTT法检测细胞增殖;羟脯氨酸试剂盒检测胶原含量;ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量;流式细胞仪检测细胞周期;硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

The article reported the effect of exogenous Ca 2+ on the proliferation of Saccharomyces cerevisiae and Schizosaccharomyces pombe .

SD培养基中外加不同浓度的Ca2 +可促进酿酒酵母与粟酒裂殖酵母细胞的增殖,但在促进增殖方式上存在明显差异。

Abstract〕 objective: To study the effects of retinoic acid on the proliferation and apoptosis of undifferentiated ectomesenchymal cells derived from facial primordia of mouse fetus.

摘要〕 目的:研究维甲酸处理未分化外胚间充质细胞后,细胞增殖和细胞凋亡的变化,探讨细胞增殖、凋亡与维甲酸之间的关系。

The 1/2 MS medium supplemented with 1.0 mg·L-1 BA and 0.1 mg·L-1 NAA was very beneficial to the protocorm differentiation and propagation of D.candidum .The highest protocorm propagation index was obtained from the medium containing the activated carbon.The highest root numbers and length were observed in plants growing in 1/2 MS medium containing 0.5 mg·L-1 NAA.

结果与结论:添加20%马铃薯液有利于种胚的萌发,种胚萌发率为79.37%;BA 1.0 mg.L-1和NAA 0.1 mg.L-1最有利于原球茎增殖,培养基中添加2%活性碳原球茎增殖倍数高;NAA有助于试管苗的生根,不同含量NAA以0.5 mg.L-1时试管苗平均根数最多和平均根长最长。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后,利用SABC法检测CD80的表达;MTT法测定肿瘤细胞体外增殖能力;将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度;在同源淋巴细胞肿瘤细胞混合培养后,通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

Prolife Feminism documents and anthologizes a strand of prolife and prowoman thinking that often goes unnoticed, especially by those so heatedly debating the legality of abortion and its connection to women's rights.

女权文件和增殖anthologizes一根增殖和prowoman以为谬论罢了,特别是那些做激烈争论的合法性及其连接堕胎妇女的权益。

Results Apototic cells emerged in the lung adenocarcinoma cells after irradiation and appeared in G1 aret subsequent to overc...

而增殖坏死可能是肺纤维母细胞照射死亡的主要形式,揭示了不同种类细胞增殖死亡的复杂性和多样性。

Proliferation of spleno cytes in duced b y both ConA and do uble-ways mixed lymphocyte cultur e.

应用MTT 法,观察雷公藤多苷对conA 诱导的脾细胞增殖反应和双向混合淋巴细胞增殖反应的作用。

TIL of human glioma is of good ability of proliferation in vitro, and this can not be affected significantly by retrovirus-mediated TNF-α gene transduc- tion into it. TIL is suitable as carrier cells for lymphokine gene therapy of human glioma.

1。人脑胶质瘤TIL具有良好的体外增殖能力,逆转录病毒中介TNF-α基因导入人脑胶质瘤TIL后,其体外增殖能力未受明显影响,符合人脑胶质瘤淋巴因子基因治疗运载细胞的要求。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

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