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Specific siRNA was designed and synthesized, and transfected Hela with liposome siNEO FX, then analyse the role of siRNA on proliferation vitality outside the Hela cells, cells periodic distribution by MTT method, double-layer soft agar clone-forming assay, and flowing cell technology.

设计并合成siRNA,脂质体siNEO FX转染Hela细胞,分别用MTT法,双层软琼脂克隆形成试验和流式细胞术分析了siRNA对Hela细胞体外生长增殖活力、细胞周期分布的作用。siRNA作用后的Hela细胞增殖速度减慢,软琼脂克隆形成率降低,G0/G1,期细胞比率增加。

Rufa Planch could restrain the cell proliferation in the stomach carcinoma SGC7901, lymphocytic leukemia L1210, mouse neuroblastomax rat gliom hybrid NG108-15, and human hepatocellular carcinoma Bele7404 cell strain by clone cells and restrain the cell SGC7901, NG108-15 cell strain by MTT.

结果山梨猕猴桃根提取物用集落形成法R6,R8对胃癌细胞株SGC7901、白血病细胞株L1210、神经性肿瘤细胞株NG108-15、肝癌细胞株Bele7404的增殖有抑制作用;用MTT法对NG108-15,SGC7901的增殖有抑制作用。

Objective To observe the effect of Seropharmacological Plastrum Testudinis on Proliferation cell unclear antigen of rat mesenchymal stem cell in vitro.

目的:观察益肾龟板血清对大鼠骨髓间质干细胞(mesenchymal stem cells, MSC)体外增殖过程的增殖细胞核抗原(Proliferation cell nuclear antigen, PCNA)表达的影响。

The process of invasion and replication of Bombyx mori cytoplasmic polyhedrosis virus in the silkworm, Bombyx mori was studied. After molting, the third instar larvae of B.

采用在家蚕活体内研究家蚕质型多角体病毒的入侵增殖复制过程,探讨BmCPV的入侵过程和增殖复制机制。

Its expression reach to peak in G1/S conversion, and in GO phase, differentiation or apolexis, the mRNA levels of MCM proteins declined to so little that can not be detected.

MCM蛋白在增殖细胞中表达水平高,在静止期细胞或分化好的细胞中不表达或水平很低,提示MCM蛋白可以作为增殖细胞的特异标志。

Some key techniques related to the close and continuous process were investigated by the application of H9N2 avian influenza virus with Vero cells, such as the susceptibility of cell to influenza virus, virus production with cell microcarrier culture method, cell bead-to-bead transfer, virus production through bead-to-bead transfer, cell culture and virus production with serum free medium, metabolism analysis, and repetitiously intermittent bead-to-bead transfer of cell for virus production to simulate the close and continuous process.

通过使用Vero细胞增殖禽流感病毒H9N2,本文针对封闭连续工艺过程的一些关键技术开展研究,包括细胞对流感病毒敏感性分析、细胞微载体培养生产病毒工艺、细胞珠到珠转移、转移后细胞对病毒增殖敏感性验证、细胞无血清培养生产流感病毒、代谢分析、可模拟连续操作的多次间歇式珠到珠转移培养细胞生产流感病毒等方面。

In conclusion ,the results of this study showed that the inducing agent HMBA could effectively inhibit the proliferation activity of the human ostosarcoma MG-63 cells,cause the arrest of G0/G1 phase, reverse the malignant phenotype characters,upregulate expression of the osteoblat-like phenotype markers including collagen,osteocalcin,osteonectin , facilitated the formation of bone nodule .

本研究的结果表明HMBA能够有效抑制人成骨肉瘤MG-63细胞增殖活动,改变肿瘤增殖核抗原表达,将细胞周期阻滞在G0/G1期,逆转人成骨肉瘤细胞恶性表型特征,在促进终末分化标志物Ⅰ型胶原纤维蛋白、骨钙蛋白、骨粘素的表达同时,促进骨结节生成,从而说明HMBA能够诱导人成骨肉瘤MG-63细胞终末分化,并且Rg1、BPO-L、Rg1+BPO-L以及Rg1+HMBA等均具有终末诱导分化能力。

The cell proliferation and differentiation were measured as index of stimulating osteoblastic bone formation. Cell proliferation was assayed by MTT method with fluoride sodium as positive control.

以成骨样细胞的增殖和分化为活性检测指标,成骨样细胞的增殖以MTT法测定,氟化钠为阳性对照药。

In vitro micropropagation of Taraxacum formosanum Kitamura was established to get maximum shoots multiplication rate by cultivating stem-node on MS medium supplemented with BA 0.6 mg/L+ NAA 0.06 mg/L and rooting rate achieved 100% by cultivating on MS and 1/2 MS medium or it supplemented with IBA 0.2 mg/L for 3 weeks.

试验结果发现龙珠顶芽优势强,在腋芽的增殖上,於BA 0.1 mg/L + NAA 0.01 mg/L之MS培养基,虽然可获得最多芽数,但产生的腋芽数只有2.3,且高度较矮,不易切芽,建议以MS为增殖培养基;在发根方面,不管在MS、1/2 MS或是添加IBA,培养4周后,发根率均为100%。

Objective: To study the dynamic changes of neointimal peoliferation,and the influence of thrombin on vascular proliferative response.

目的:探讨内皮拉伤后血管内膜增殖反应的动态变化,和凝血酶对损伤血管增殖反应的影响。

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