增殖的

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

It was found that Pelargonium citrosum Vanleenii proliferation period can be prolonged in proper concentration of Ca~(2+).

结果表明：适当提高Ca2+的浓度，可以延长驱蚊香草试管苗的正常增殖生长周期；不同浓度的Ca2+处理对驱蚊香草试管苗增殖的影响要大于对其生根的影响；驱蚊香草试管苗体内Ca2+含量与试验培养基中Ca2+浓度成线性正相关。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞，建立小鼠T淋巴细胞活化、增殖的模型，以不同浓度的CPT作用于该模型，流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂，以不同浓度的TMB-8及与环孢菌素A联合作用于该系统，用流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术，分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色分析细胞周期的分布情况。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型，通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用；采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用；利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

The germinative number of horsebeans and peas were raised by 44% with activated water by tourmaline. 200μl 1 X 10~6/ml mouse's H_(22) cell, 200μ 4X 10~6/ml splenic cell, 200μl 1 × 10~6/ml epidermal cell were all inhibited after the action of tourmaline. It is analyzed that the factors which effected the multiplication of the cells included direct action which are tourmaline's electric field, far-infrared, negative ions and solvent ions, and indirect effect which are small water clusters and pH value.

电气石活化水使蚕豆和绿豌豆种子发芽速度均提高44%。0.5g电气石分别对200μl 1×10~6/ml的小白鼠H_(22)肝癌细胞，对200μl 4×10~6/ml小白鼠脾细胞，对200μl 1×10~6/ml的小白鼠表皮细胞的增殖起抑制作用，分析认为，电气石影响细胞增殖的因素包括电场、红外线、负离子和溶出离子对细胞的直接作用和通过改变水分子结构、培养基pH值的间接作用。

Methods: 5% propranolol emulsion was applied externally on the back of a guinea pig\' s ear to produce the psoriasis-like dermatitis model to observe the effects of Baibi ointment on the histopathological changes of the model.Radioimmunoassay was used to observe the levels of cytokines like IL-2、IL—6 and IL-8, and the effects of the ointment to the cytokines. MTT method was used to study the effects of the extracts of the Baibi Formula and the extracts of its main components Huangjing , Kushen , Dahuang ,Huangbai, to the multiplication of HaCaT cells—one of the Malpighian cell line with and without stimulation of TNF-α.

实验采用5%心得安乳剂建立豚鼠银屑病样皮炎模型，观察白疙软膏对豚鼠银屑病样皮炎模型组织病理改变的影响；采用放射免疫法检测豚鼠银屑病样皮炎模型皮损中细胞因子IL-2、IL-6、IL-8的水平，观察白疕软膏对豚鼠银屑病样皮炎模型皮损中细胞因子的影响；采用MTT法研究了中药白疕方提取液及白疕方主要组成药物大黄、黄柏、黄精、苦参提取液对角质形成细胞系HaCaT细胞增殖的影响及对TNF-α刺激后的角质形成细胞系HaCaT细胞增殖的影响。

Result 1. CDK4, P16 were expressed in parabasal layers and the upper layer, mainly in the prickle and granular layers of cholesteatoma epithelium. Their positive rates were 68.6% and 88.6% respectively. Statistical analysis showed that there was a significant difference in positive rates between

CDK4与P16在中耳胆脂瘤上皮中均过度表达且有一定程度的正相关性，表明它们在疾病发生中是共同而非单独起作用的，从细胞周期的角度可反映胆脂瘤上皮细胞过度增殖的同时也增强负调控因子来抑制增殖，使凋亡同时加快而达到新的平

In Expt.1, that the effects of microbial inoculants and formic acid on the pH, the number of LAB, yeast and mold, and the formation of lactic acid was evaluated during the different periods of corn silage (after day0.5, 1,2, 3,5, 7, 10, 20, 40 and 60 of ensiling) using the methods of laboratory silos and microbiology techniques.

原因在于本试验接种的乳酸菌数量较少，发酵过程中没有成为优势菌群。添加甲酸在发酵初期抑制了乳酸菌的增殖，降低了乳酸的生成量和青贮初期的pH值，提高了青贮后期pH值；对酵母和霉菌最初表现为促进增殖的作用，5天以后对酵母有较强的抑制作用。

Then PLGA Micro spheres can promotes the proliferation of osteoblast in a long perlod because of the controlled release of bFGF from microspheres. The PELA microspheres satisfied the proprty reguirement of controlled release, but the biological activity of the released bFGF is partially destroyed.

2体外细胞试验中，游离bFGF对成骨细胞具有促分裂增殖的作用，但效应期很短；而PLGA微球通过持续释放活性bFGF，可在较长时期内促进成骨细胞的分裂增殖；PELA微球虽然达到了缓释的性能要求，但释放出的bFGF的生物活性受到了破坏。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。