增殖的

Methods: Virus proliferation assay, cell viability assay and Western blot were performed to assess the selective replication and cytolysis of CNHK500 in telomerase positive liver cancer cells Hep3B, HepGⅡ, SMMC7721 and in normal cells.

方法利用病毒增殖实验、细胞活力实验、蛋白印迹分析来检测增殖病毒CNHK500在端粒酶阳性的肝癌细胞株HepGⅡ、Hep3B、SMMC7721及正常细胞中选择性增殖和溶解细胞的特性。

Primary operation cases of parotid pleomorphic adenoma have more proliferative activity of Ki-67 than nonrecurrent group, less than group of low grade malignant parotid gland tumors. Partial parotidectomy is still suitable for primary operation cases of parotid pleomorphic adenoma.

腮腺多形性腺瘤手术复发前者Ki-67增殖活性高于未复发者，但与低度恶性腮腺肿瘤比较，其增殖活性明显不同，因此手术方式仍可选择腮腺部分切除术，但应结合其组织病理学和增殖活跃的特点，加强术后随访。

To investigate the effect of p38MAPK signal conducting pathway on livercancinoma cell's malignant phenotype induced by VEGF,we take theexperiment with cell growth test,scanning microscope and laser scanningconfocal microscope so that observe effect of p38MAPK signal conductingpathway on liver cancinoma cell growth,pseudopodium formation andframework of cytoskeleton induced by VEGF.results indicate that the cellbecame ellipse and there were more and thick pseudopodium in the cell'ssurface after being treated by VEGF,and destroyed framework ofcytoskeleton,which can be blocked by pretreated with a special inhibitor ofp38MAPK SB203580 so that VEGF promote metastasis by enhancing livercell migration and movement via p38MAPK signal conducting pathway,butVEGF promote cell growth without p38MAPK signal conducting pathway.

为进一步探讨VEGF通过p38信号传导通路诱导肝癌细胞转移时，对肝癌细胞恶性表型的影响，采用细胞增殖实验、扫描电子显微镜、激光扫描共聚焦显微镜观察VEGF对肝癌细胞增殖作用、细胞伪足形成、细胞骨架微丝结构的影响以及p38MAPK信号通路调控作用。结果显示，VEGF能够不通过p38信号通路促进肝癌细胞增殖；VEGF诱导肝癌细胞丝状伪足增多、增长，使细胞骨架微丝结构破坏、甚至消失。阻断p38MAPK信号通路，可以抑制VEGF诱导的肝癌细胞伪足形成，细胞骨架丝状结构呈束状，排列较规整。上述结果表明，VEGF可以通过p38信号传导通路诱导肝癌细胞伪足增多、增长，促使细胞骨架微丝结构破坏，使肝癌细胞迁移、运动能力增加，促进肿瘤转移。VEGF并不通过p38信号通路诱导肝癌细胞增殖作用。

The virus was purified by ultracentrifuge. According to the NS gene sequence published by Genbank, One primer T-1 was designed to amplify the cDNA by reverse transcript.

本实验用接种鸡胚的病毒增殖方法获得了A/Chicken/Mudanjiang/0823/00(H9N2)分离株的大量增殖，增殖病毒经差速离心进行纯化，经超迷离心浓缩。

He CD4〓 T-cell viability determined by the trypan blue test was over 90%. PBMCs from the recipients, after inactivated, were added in 96-well culture plates with donor CD4〓 T-cell cocultured at 37℃ for 24 hours, TJU103 and CTLA4-Ig were given for additional 48 hours coculture. The CD4〓 T-cell was collected and washed as anergic CD4〓 T-cell.

JU103联合CTLA4-Ig应用对供者T细胞增殖活性的影响根据TJU103有剂量限制性特点，选择TJU103最大抑制效应浓度50μg/ml，与不同浓度CTLA4-Ig（1μg/ml、10μg/ml、50μg/ml、100μg/ml）联合，对供者T细胞的增殖抑制率分别为（59.5％、90.5％、93.4％、95.8％），TJU103（50μg/ml）与低浓度CTLA4-Ig（10μg/ml）联合，增殖抑制率在90％以上，已接近最大增殖抑制率，为二者联合应用的最佳组合，与单独应用组比较，有显著的统计学差异（p＜0.05）。

Proliferating cell nuclear antigen is usually regarded as anindex of determinating cell proliferating and its expression levelin the nuclear is correlated with with the activity of cellproliferation. The activity of tumor cell proliferation can beknown by examinationing PCNA of tumor cells.

增殖细胞核抗原(proliferating cell nuclear antigen，PCNA)常作为测定细胞增殖水平的指标，其在细胞核内阳性表达的高低与细胞增殖活性密切相关，通过检测肿瘤细胞的PCNA ，可了解和评价肿瘤组织的增殖活性。

Results When the culture medium was supplemented with 0 .05μg/ml sodium selenite,the proliferation of transformed cells was significant ly promoted and the transformation did not change remarkably,when supplemented w ith 0.25μg/ml,the proliferation was promoted but the transformation was inh ibited,and when with 0.75μg/ml and 1.50μg/ml,the proliferation was not pro moted and the transformation was strongly inhibited.Big dose of (1.50μg/ml) sodium selenite m ade the nuclei of the untransformed lymphocytes gather together like a few grape s doing,and even caused them to fuse.

结果 在培养物中添加0.05μg/ml亚硒酸钠能显著促进转化细胞增殖；添加0.25μg/ml可促进转化细胞增殖，但显著抑制细胞转化；添加0.75μg/ml和1.50μg/ml，不仅不能促进细胞增殖，而且强烈抑制细胞转化。1.50μg/ml剂量的亚硒酸钠使非转化淋巴细胞发生葡萄状聚集甚至核融合。

The vein grafts with perivenous support by fibrin glue demonstrated a statistically decrease in neointimal and medial hyperplasia compared with non-supported vein grafts（p.01）. immunohistochemical analysis showed that the expression of proliferating cell nuclear antigen was inhibited significantly in fs group compared with ns group. almost intact endothelium was showed in fs grafts（p.01）. but in ns grafts there were severe endothelium damages where lymphocytes infiltrated. in ns grafts, the smooth muscle cells were hyperplasia and migrated to intimal, whereas in fs group, the hyperplasia of smooth muscle cells were inhibited and migrated to adventitia.

免疫组化结果显示与ns组相比，fs组移植静脉细胞增殖核抗原表达明显受到抑制；ns组内皮层损伤严重，有淋巴细胞侵润，而fs组内皮层则几乎完好无损（p.01）；在ns组平滑肌细胞增殖活跃且向内膜迁移，fs组平滑肌细胞增殖受到抑制，且向外膜迁移；在fs组移植静脉外膜有丰富的成熟微小血管网形成，但是在ns组，微小血管数量则非常少。

Pella R. The protective effect of atrial natriuretic peptide on cells damaged by oxygen radicals is mediated through elevated CGMP-levels, reduction of calcium-inflow and probably G-proteins.

心肌肥厚是细胞促增殖和抑增殖因素失去平衡的结果，以往的研究多集中于促增殖因子如ET-1、 AngⅡ等，但是从治疗的角度看，对抑增殖因子的研究具有更重要的意义。

There was significant difference in T lymphocyte proliferation between the TF treatment groups and the control groups (p＜0.01), and between TF from black-bone silky fowl and other treatment groups (p＜0.01). Significant difference was detected between TF from black-bone silky fowl and homogenous Cherry Valley duck (p＜0.01). The effect on T lymphocyte proliferation in vivo was markedly enhanced when taking oral TF in 24 h, and significant difference remained between the treatment groups and the control groups in 240 h.

实验结果进行t检验分析，各实验组TF与对照组数据进行比较极显著差异，p＜0.01；乌骨鸡来源TF与其他实验组数据进行比较极显著差异，p＜0.01；T细胞体内转化增殖实验结果表明TF口服作用24 h内极显著提高T细胞转化增殖效果，而且240 h内T细胞的增殖效果与对照组相比仍然保持极显著差异。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷