增殖的

3While in denervated muscle, actinomycin D inhibited proliferation of satellite cells asw...

上述结果提示：肌肉的卫星细胞增殖和胞纳增加可能发生于去神经后某些因素的出现，或者胞纳的增加即是卫星细胞增殖的结果。

The PKC family is subdivided into three groups; the classical PKC members are Ca〓 and diacylglycerol dependent, the novel PKCn are Ca〓-independent but diacylglycerol dependent and the atypical PKCs are not activated by Ca〓and diacylglycerol in vitro.

丝裂素活化蛋白激酶是一族在脊椎类动物体内广泛存在的蛋白质丝氨酸/苏氨酸激酶，是目前发现的最主要的一个生长信号调节蛋白，是细胞内多种促细胞增殖信息传递的共同通路或称会聚点，是细胞表型变化和增殖的蛋白激酶。

The study was purposed to investigate the effect of extract of Agkistrodon Halys venom on proliferation and apoptosis of K562 cells. The inhibition of K562 cell proliferation was measured by MTT assay; The morphologic changes of K562 cells was observed by microscopy; the apoptosis of K562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase in K562 cells was detected by Western blot.

本研究探讨皖南蝮蛇毒蛋白提取物对白血病细胞K562增殖的影响及诱导凋亡的作用，采用MTT法检测不同浓度的蛇毒蛋白提取物对K562细胞生长的影响；应用电子显微镜观察药物作用后K562细胞形态的改变；流式细胞术检测蛋白提取物对K562细胞细胞凋亡作用；Western blot检测细胞增殖酶活性的变化。

Morin can significantly inhibit ConA stimulated activation and proliferation of murine T lymphocytes, in which the S phase lagging may serve as one of the major mechanisms.

Morin可显著抑制ConA刺激的T细胞活化及增殖；其对增殖的抑制作用主要表现为S期的细胞的阻滞。

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数按摩明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Result: CDK4 and P16 were expressed in parabasal layers and the upper layer, mainly in the prickle and granular layers of cholesteatoma epithelium.

CDK4与P16在中耳胆脂瘤发生中是共同而非单独起作用的，从细胞周期的角度可反映胆脂瘤上皮细胞过度增殖的同时也增强负调控因子来抑制增殖，使凋亡同时加快而达到新的平衡。

Methods The cells of LLC-PK1,Hela,Caki-1,T24 and fibroblast were incubated on 96-w ell plate with addition of diffe-rent concentration of cystic fluid of ACDK and t hat of simple renal cyst. After 24 hours for reaction, MTT method was used to de termine and compare the proliferative activity of 5 cell lines.

将LLC-PK1， Hela， T24， Caki-1和成纤维细胞接种于96孔培养板中，经过24h培养，加入不同浓度的ACDK和单纯肾囊肿的囊内液作用24h，用MTT法测定培养细胞的增殖活性，比较两者之间对细胞增殖的抑制作用。

Telomerase activation is a key tumor marker which immortalizes cell.If a medicine blocks telomere prolonging by attenuation of telomerase activity, it could inhibit tumor cells proliferation.

因为端粒酶活化是大多数肿瘤细胞维持端粒长度并获得永生化的必要条件，如果某种药物能抑制端粒酶的活性就可阻断端粒的合成，从而使细胞退出增殖周期，达到抑制肿瘤细胞增殖的目的。

Based on the above findings, it could be concluded that:(1) berbamine could obviously inhibit the cell proliferation and induce apoptosis in four different kinds of leukemic cell lines (including K〓, Jurkat, NB〓, HL〓 cells) in a time-and-concentration-dependent manner in vitro.

综上所述，我们认为：(1)钙调素拮抗剂小檗胺在体外除对HL〓细胞外，还对其它3种不同类型的白血病细胞株(K〓、Jurkat、NB〓)细胞具有明显的增殖抑制作用及明确的诱导凋亡作用，并呈时间-浓度依赖关系，诱导细胞凋亡可能是其抑制白血病细胞增殖的主要方式之一。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。