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In order to discuss the optimal condition in which GPMV proliferates in chicken embryo and its pathogenicity, GPMV of different titer was inoculated into 911-day chicken embryo and the allantoic fluid was harvested after 36, 48, 60, 72 and 84 h, the HA titer was measured as well.

为了探讨鹅副粘病毒在鸡胚中增殖的最佳条件及其致病性,将不同血凝滴度的鹅副粘病毒分别接种于9~11日龄鸡胚尿囊腔,收获孵化36、48、60、72和84 h的鸡胚尿囊液,测定血凝滴度。

RESULTS: Treated for a same period (3 d), the inhibitory effect of MG132 on PC12 ceil proliferation enhanced with the increment of the concentration of MG132(0, 1, 2.5, 5, 10, 20 μmot/L).

用不同浓度的MG132处理PC12细胞3d,通过3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐微量比色法检测MG132对PC12细胞增殖的影响,通过姬姆萨染色法和流式细胞术检测MG132对PC12细胞凋亡的影响。

Objective To study the effect of monocyte chemotactic protein-1(MCP-1) on the proliferaion of tumor infiltrating lymphocytes in patients with transitional cell cancer of bladder and their cytoxicity to bladder tumor.

目的 探讨不同浓度的单核细胞趋化因子(MCP 1)对肿瘤浸润淋巴细胞增殖的作用,以及能否增加TIL对膀胱肿瘤细胞的细胞毒性。

Objective: To study proliferation of mesenchymal stem cells affected by different concentration of bFGF ,and influenced by bFGF with TGF-β 1 in vitro. To evaluate chondrogenesis of MSCs affected by growth factors and time.

目的:研究碱性成纤维生长因子的不同浓度及同转化生长因子(TGF-β_1)联合作用,对兔间充质干细胞(Mesenchymal Stem Cells,MSCs)增殖的影响,研究不同因子和培养时间对兔MSCs体外软骨形成的影响。

Then the cell proliferation activity was detected using MTT colorimetrics, the cell cycle and apoptosis after LPS stimulation using flow cytometry, and expressions of caspase3, Bcl2, Bax using immunocytochemical staining.

mg/L的LPS作用于细胞,采用MTT染色法检测LPS对细胞增殖的影响;采用流式细胞术检测细胞周期及凋亡;采用免疫组织化学染色技术检测细胞的caspase3、Bcl2、Bax表达变化。

Because of the colon, prostate, breast and some other organizations may have enzymatic functioning mechanisms, they can be 25 hydroxy vitamin D into 1,25 dihydroxy vitamin D, which is a powerful hormone regulating cell proliferation, and may restrictions on certain types of cancer metabolic activity.

因为结肠、前列腺、乳腺和其他一些组织可能拥有酶促运转机制,它们能将25羟维生素D转化成1,25二羟维生素D,后者是一种强有力的调节细胞增殖的激素,并且可能限制某些癌症的代谢活性。

Objective: To explore the inhibition effects of ectogenic wild-type p53 cDNA (Ad-wtp53) on colorectal carcinoma cell lines with different p53 gene status and search for the role of wild type p53 tumor suppressor gene in occurrenc and progress of malignant tumor.

目的:探索重组腺病毒介导的外源性野生型p53cDNA(Ad-wtp53)对内源性p53基因状态不同结直肠癌细胞的抑制作用是否存在差异,研究p53基因抑制肿瘤细胞增殖的机制。

HepG2 cells were exposured to oxymatrine, final concentrations of which were 0.1, 0.2, 0.3, 0.4, 0.5, 0.8, 1, 2, 5, 8, 10, 15 g.L-1 respectively, MTT method was used to determine the inhibiting effects of oxymatrine on HepG2 cells proliferation, followed by Haematoxylin and Eosin staining to observe the cell morphology.

以0.1,0.2,0.3,0.4,0.5,0.8,1,2,5,8,10,15 g.L-1的氧化苦参碱处理HepG2细胞,四噻唑蓝法检测其对细胞增殖的影响;苏木精-伊红染色比较细胞形态差异;免疫组化染色进一步检测HepG2细胞肿瘤相关蛋白表达量的变化。

Objective To investigate whether novel isoflavone and steroid derivatives have better antitumor activity.

目的 研究改构后的异黄酮及甾体类化合物是否具较好的体外抗细胞增殖的活性。

It is indicated that the ,mechanism of topical treatment with calcipotriol in psoriasis involves not only inhibiting keratinocyte proliferation, but also effecting ICAM-3 positive infiltrating leukocytes in lesional skin, which may be involved in the pathogenesis of psoriasis.

外用钙泊三醇治疗银屑病的机制除了有抗角朊细胞增殖的作用外,对可能参与银屑病发病机制的ICAM-3阳性浸润细胞也有作用。

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