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Objective: To make a research of Marsdenia Tenacissima extracts on proliferation effect of HepG2 in vitro.

目的:研究乌骨藤提取物体外对培养的人肝癌细胞(HepG2)增殖的影响。

The optimum work concentration of the indirect ELISA for detecting and screening positive hybridoma cells was established by research again and again. Polystyrene microtitration plates were coated with the purified DHV antigen at the amount of 0.5μg/well.

对鸡胚和鸭胚增殖的尿囊液病毒用反复冻融、氯仿处理、0.22μm滤膜过滤、PEG反透析浓缩以及Sephadex G-200分子筛层析的方法进行纯化,并以此来包被酶标板以及进行BALB/c小鼠免疫,效果良好。

Methods The polysaccharide was extracted and purified from Millettia Speciosa Champ,CFDA-SE staining and flow cytometry were used to detect the effects of polysaccharide on mouse lymphocyte proliferation stimulated by Con A.

提取、纯化牛大力多糖,利用CFDA-SE染色,流式细胞术检测多糖对Con A刺激引起的小鼠T淋巴细胞增殖的影响。

MHC class Ⅱ,CD40,CD80,and CD86 on DC were measured using flow cytometry.Results The ability of DC to stimulate nonadherent splenocytes proliferation was decreased after injury,and the levels of cytokines were changed.

致伤后24小时分离小鼠脾脏DC ,检测脾脏DC在体外抗原作用下刺激脾非黏附细胞增殖的能力、细胞因子分泌功能及其表面重要分子表达的情况。

The level of β-tp was in direct proportional to the proliferation of cell in the oligodendroglioma cell lines (SHG-44, U-251, BT-325), but not in the non-oligodendroglioma cell lines (oophroma, prostate-carcinoma and neuro-oocyte).

但在非胶质瘤细胞株,如卵巢癌细胞,神经母细胞瘤细胞及前列腺癌细胞株等,β-tp的浓度与细胞增殖的趋势相反。

These results showed that there were significantly different effects of AGE-BSA on cell proliferation between human and rats' osteoblastic cell.

以上结果表明:AGE对人和大鼠的成骨细胞增殖的诱导作用相差很大。

AIM: To study the effects of simvastatin on osteoblastic differentiation and proliferation of human bone marrow stromal cells, and investigate the mechanism of the effects of simvastatin on hBMSC.

目的:通过对成人骨髓基质细胞体外培养的观察,研究辛伐他汀对hBMSC成骨分化,增殖的影响和作用机制。

Third: for understanding the effect of c-myc AS ODN on pannus formation in synovium, we investigated the effect of c-myc AS ODN on endothelial cell line—ECV304 cells proliferation.

了解c-myc AS ODN是否对滑膜中的血管翳形成具有抑制作用,观察c-myc AS ODN对血管内皮细胞系ECV304细胞增殖的影响。

AIM: To investigate the antineoplastic effect of pholiota nameko polysaccharides and the mechanism of inducing K562 cell apoptosis. METHODS: MTT assay was used to determine the inhibitory rate of PNP with different concentrations on the proliferation of K562 cells, and the cell growth curve was drawn.

采用MTT法检测PNP对K562细胞增殖的抑制作用;绘制生长曲线;Hochest 33258染色计算凋亡率;RTPCR法、Western Blot方法检测凋亡相关基因Caspase3的表达。

Objective: To study the relationship of HIF-1α expression and proliferative activity in meningioma.

目的:探讨缺氧诱导因子-1α的表达与脑膜瘤细胞增殖的关系。

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