增殖的

To investigate the expression of COX-2 in condyloma acuminatum and its relationship with the proliferation in tissues.

目的探讨环氧化酶-2(COX-2)在尖锐湿疣组织中的表达及与细胞增殖和血管增殖的关系。

Carotid artery samples of control and Doxy treatment groups underwenthistological and morphometric analyses at 1,3,7,14,28 days after injury respectivly, and wemeasured the thickness and area of neointimal, lumen area and the proliferation of SMCsusing a computer-assisted morphometric analyses system.

于术后 24h、3d、7d、14d、28d 原位灌注固定颈动脉后取标本，采用 HE 染色、VVG 染色、免疫组化标记 a-actin 和增殖细胞核抗原以及透射电镜的方法观察损伤动脉内膜厚度、管腔重构及 SMC 增殖的情况。

Objective To investigate the effect of hypoxia on proliferation of rat embryonic mesencephalon-derived NSCs and its mechanism.

目的 观察低氧对大鼠中脑神经干细胞体外增殖的影响，并探讨其增殖的可能机制。

In order to discuss the optimal conditions of Nostoc flagelliforme multiplication, the effect of types of medium, pH, temperature, light period, light intensity and hormone on growth and multiplication of Minor flagelliforme cells were investigated.

为探讨发菜生长增殖所需的适宜条件，研究了培养基类型、pH值、温度、光周期、光照强度和激素等对发菜细胞生长增殖的影响。

Igniarius were carried out. It will be of scientific significance in researching and developing rare medicial fungi and be useful for searching new natural antitumor medicine.The crude polysaccharides of P. igniarius PIP30、PIP60、PIP80 and PIWE1-10、PIWE10-100、PIWE100 were obtained by ethanol precipitation and ultrafiltrate respectively after hot water extracting.

首先对桑黄子实体多糖进行了分离纯化，通过热水提取、乙醇分级沉淀分离得到粗多糖PIP30、PIP60和PIP80，通过超滤分离得到粗多糖PIWE1-10、PIWE10-100和PIWE100；6种粗多糖经体外刺激淋巴细胞增殖实验表明均有体外刺激淋巴细胞增殖的作用。

Apoptosis and changes in cell cycle were examined by means of flow cytometry. P21(superscript waf/cif1) mRNA expression was assessed by RT-PCR.

结果：Compound和HCPT在微摩尔级浓度即能有效抑制BIU-87细胞增殖，而Compound抑制BIU-87细胞增殖的效果更明显。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Results: A 2-AAF/PH model was successfully established to activate the oval cell compartment in rat liver. HOC appeared mainly in periportal areas and could extend into the liver parenchyma. The volume of oval cells was about as big as one forth to one sixth of normal liver cells, with a oval-shaped nucleus and high nuclear/cytoplasm ratio.

结果：以2-乙酰氨基芴加三分之二肝切除术成功建立了大鼠肝卵圆细胞增殖模型，HE染色汇管区可见大量增殖的小圆细胞，并可向肝实质内延伸，免疫组化证实增生细胞OV-6、c-kit、CKl9及AFP染色阳性，而白细胞共同抗原染色阴性。

Objective: To understand the differentially gene expressions of nasopharyngeal carcinoma CNE-2 cell lines in different proliferative status and to investigate the mechanism of accelerated repopulation in NPC during irradiation.

目的：了解不同增殖状态鼻咽癌细胞（CNE-2）细胞周期基因表达差异，探讨鼻咽癌放射治疗中加速再增殖的机理。

Cell growth pattern and its responses to cytokines were evaluated by trypan blue exclusion and MTT test, respectively.

台盼蓝拒染法测定细胞增殖状态，MTT实验检测不同细胞因子对细胞增殖的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。