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The ultrastructures of amyloplast development and proliferation in maize endosperm cell were observed using the method of glutaraldehyde-osmium tetroxide fixation.

采用戊二醛+锇酸双重固定法观察了玉米胚乳细胞淀粉质体发育和增殖的超微结构。

The ultrastructures of amyloplast development and proliferation in endosperm cell of maize were observed using the method of glutaraldehyde-osmium tetroxide fixation.

采用戊二醛+锇酸双重固定法观察了玉米胚乳细胞淀粉质体发育和增殖的超微结构。

Effects of explants,2,4-D,6-BA,NAA and AgNO3 on inducing buds were studied in pineapple(Ananas comosus cv.Hawaii) by using orthogonal design.

采用菠萝试管苗的叶片、叶鞘、茎段和茎薄片作外植体,以MS或1/2 MS为基本培养基,应用正交试验设计方法研究了不同浓度的2,4-D、6-BA、NAA和AgNO3对夏威夷菠萝不定芽的诱导效果,并在添加不同激素组合的培养基中进行芽的增殖和生根培养研究。

This paper summarized the technology progress of tissue culture of Anthurium andraeanum, included the sources of explants,the different culture mediums, the sort and content of the hormones, the different carbon source.

文章介绍了在红掌的组培快繁方面的研究进展,对外植体的选择,不同基本培养基、激素、碳源对其增殖与分化的影响,以及生根壮苗的方法等进行了综述。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子,以色谱法分离纯化后进行体外活性研究,构建表达载体pPICZαA- ALR,经电穿孔转入毕赤酵母中,用0.5%甲醇诱导表达;重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明, rhALR以分子量为30kD的二聚体为主;定量分析结果表明,重组酵母培养上清中rhALR约占总蛋白的66%,表达量约为40mg/L;经DEAE柱和G75柱纯化后,获得的rhALR纯度大于95%,得率为52%;体外生物学活性实验表明,rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Investigation of formation, proliferation and degradation of the peroxisome is an important part of study of organelles biogenesis.

过氧化物酶体(peroxisome,P)是真核细胞中普遍存在的细胞器,参与多种重要的代谢过程。P的产生、增殖及降解是细胞器发生机理研究的重要部分。

Through a study on tissue cultureof the bud scale of corm of Lili longiflorum separated from the plant ,several practical TC prompt proliferation techniques ,such as inducing of lush bud ,proliferation cultivation of successive generation, cultivation of growing roots and transplanting , etc., are researched in this paper .

通过对百合球茎鳞片离体组织培养的研究,探讨了丛生芽诱导、继代增殖培养、生根培养及移栽等方面的组培快速繁殖以及生产上实用的组培技术。

Consistent with predominantly stochastic gene expression and increased energy demands induced by aneuploidy,the "buffering" canalization processes in Down syndrome would be reduced thereby giving rise to increased variance to less stable developmental pathways such as proliferation.

与非整倍体诱导的主要预测基因的表达和增长的能量需要相一致,Down综合征中的"缓冲"的发育稳态过程将会减少,从而导致像增殖这样不稳定的发育途径的多样性增加。

It turned out that hematopoietic stem cells be immortalized by constitutive Notch1 and Delta has the function as the mitogenic regulators of hematopoietic stem cells.

研究人员将活化形式Notch受体导入造血干细胞,使其获得了细胞因子依赖性的"永生";另外,Notch的配体Delta、Jagged具有类有丝分裂因子作用,可保持造血干细胞的自我更新能力,促进其体外增殖。

These culture conditions not only maintain the undifferentiated state and normal diploid karyotypes of BALB/c mouse ES cells effectively, but also maintain a series of their characteristics of murine stem cells. two different kinds of digestive methods and Two kinds of digestive juice with different concentrations were designed to dissociate proliferous inner cell mass and ES cell clonies deriver from dissociative ICM.

这一培养条件不但有效地维持了ES细胞的未分化状态和正常二倍体核型,而且维持了其作为多能性胚胎干细胞的一系列特征;实验设计了两种离散方法和两种浓度的消化液,用来离散增殖的ICM和ICM离散后出现的ES集落。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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