增殖体

In addition, the role of the RER in protein body forming, the role of amyloplast envelope in amyloplast proliferating, the relations between nucleus and cytoplasm of starchy endosperm cells in rice, and the variation of nucleus in plant PCD, the roles of plasmalemma invagination, ER, Ca〓 and phosphatases during endosperm development were discussed.

本文对粗面内质网在蛋白体形成中的作用，淀粉质体被膜在淀粉质体增殖中的作用，淀粉胚乳细胞的核质关系，植物PCD中细胞核的变化，以及质膜内陷、内质网、Ca〓和磷酸水解酶在胚乳发育中的作用等进行了讨论。

These results suggested that many organella could all develop into amyloplast, variation of amyloplast envelope resulted in amylopast proliferation, RER played an important role in synthesis and deposition of protein, ER showed many functions during endosperm development, intercellular space and plasmalemma invagination played an important role in transportation of nutrients, degeneration of starchy endosperm nucleus was a special PCD, Ca〓 and phosphatases played an important role during endosperm development.

上述结果表明：多种细胞器可以趋同发育成淀粉质体，淀粉质体增殖来源于其被膜的活动，粗面内质网在蛋白质的合成与积累中具有重要作用，内质网在胚乳发育中具有多种功能，胞间隙和质膜内陷在营养物质的运输中具有重要作用，淀粉胚乳细胞核的衰退是一种特殊形式的编程性死亡，Ca〓和磷酸水解酶在胚乳发育中起重要作用。

Methods immunophenotype and dna ploidy of the bone marrow and peripheral blood samples was analyzed by means of the flow cytometer with 3-color fluorescence. results of 52 al patients, 28 were patients with diploid, 22 were patients with heteroploid and 2 were patients with suspected heteroploid.

采用流式细胞仪和dna检测试剂盒检测急性白血病患者dna倍体、合成期细胞百分比、增殖指数并与dna倍体分析对照组进行比较。

This paper reports something about it .The callus was induced and proliferated by using young stem , leaf and leafstalk as explants .which came out in early spring . In this paper , its effects on induction and proliferation were discussed by using different kinds of explants and hormone and different physical and chemical factors.The growth circle and some physiological and biochemical factors were studied during the culture of callus, a set of rapid propagation techniques were founded, which was rapid, efficient and steady.

本文以丹参初春萌发的幼茎、叶、叶柄为外植体，探讨了不同的外植体、不同的植物生长调节物质、不同的理化因子对愈伤组织的诱导和增殖效应，研究了黑暗和光照条件下愈伤组织细胞培养生长周期以及生长周期中生理生化指标的变化特征，摸索了一套快速、高效、稳定的丹参快速繁殖技术，为丹参组织培养、次生代谢物的生产提供理论依据，为丹参快速繁殖应用于生产提供技术支持。

Vitrification, chlorosis, scale-shaped differentiation and failure to root of regenerated shoots frequently occur in industrialized production of tetraploid watermelon Citrullus vulgaris L.

西瓜四倍体工厂化生产中存在试管苗玻璃化、黄化死亡、鳞片状分化、不生根等不利问题，在MS中NH4NO3 含量减半的情况下，前两者可得到有效的控制；用连续增殖、连续壮苗的方法，解决了西瓜四倍体鳞片状分化的难题；用茎尖扩繁的最佳培养基为MS(含半量NH4NO3 )+2 。

Results In Vero cell system,under lucifugal and illumination conditions,fullerene C60 has direct virucidal activity and infection block effect,but it does not has any multiplication inhibition action to HSV-1F strain.

以逆相蒸馏法合成了富勒烯脂质体，用药物对病毒所致细胞病变的抑制实验以及病毒的空斑减数实验，观察C60脂质体对HSV-1F株的直接杀灭、感染阻断及增殖抑制作用。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The flow cytometry was used to measure the DNA ploidy type and cell proliferous ratio of NP,BPH and PC.

PC组异倍体率为65.71%；NP组和BPH组均为DNA二倍体。PC组细胞增殖各期比值与BPH组、NP组差异有显著性(P.01)。

Objective To investigate the potential pathogenicity of Mycoplasma penetrans (M. penetrans), and to study nitric oxide production and cell prolification in lipid-associated membrane proteins-stimulated mouse macrophages.

目的 检测穿透支原体脂质相关膜蛋白能否诱导小鼠巨噬细胞产生一氧化氮及其对细胞增殖的影响，以了解穿透支原体潜在的致病性。

Result: With a high cancer-inhibiting rate on the animal model having been transplanted the cancer, this medicine can remarkably restrain the growth of cancer cells cultivated in vitro and improve the life quality of mouse model, and also shows obvious promotion and enhancement function on the DTH ear-swelling of mouse with cancer, the proliferation of mouse spleen lymphocyte induced by ConA and the cytokine (TNF-α、IFN-γ) of mouse with cancer respectively. It can obviously decrease the adverse effects like weight reduction, damage of viscera and degradation of life quality caused by the chemotherapeutic medicines, and has a remarkable stimulation on the lymphocyte proliferation induced by ConA. It is also able to improve the ratio of SMMC-7721 cells in G0-G1 period and reduce the cell amount in G2/M period to cut down the possibility of stepping into the division period; additionally, it can obviously inhibit some cancer genes of the cancer cells and meanwhile up regulate the cancer-inhibiting gene.

结果：本品能明显抑制体外培养肿瘤细胞的生长，对在体肿瘤移植模型动物具有较高的抑瘤率，能明显提高模型小鼠的生存质量；对荷瘤小鼠DTH耳肿胀、ConA诱导的小鼠脾淋巴细胞增殖、荷瘤小鼠细胞因子（TNF-α、IFN-γ）呈现明显的促进或提高作用；能显著减少化疗药所引起的小鼠体重降低、脏器损害以及生存质量下降等不良作用；本品含药血清对ConA诱导的淋巴细胞增殖具有明显的促进作用；能提高SMMC-7721细胞G0-G1期比例，降低G2/M期细胞数目，而减少其进入分裂期；对肿瘤细胞的多个癌基因具有明显的抑制作用，同时上调抑癌基因等。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失