增殖体

Specific siRNA was designed and synthesized, and transfected Hela with liposome siNEO FX, then analyse the role of siRNA on proliferation vitality outside the Hela cells, cells periodic distribution by MTT method, double-layer soft agar clone-forming assay, and flowing cell technology.

设计并合成siRNA，脂质体siNEO FX转染Hela细胞，分别用MTT法，双层软琼脂克隆形成试验和流式细胞术分析了siRNA对Hela细胞体外生长增殖活力、细胞周期分布的作用。siRNA作用后的Hela细胞增殖速度减慢，软琼脂克隆形成率降低，G0/G1，期细胞比率增加。

The process of invasion and replication of Bombyx mori cytoplasmic polyhedrosis virus in the silkworm, Bombyx mori was studied. After molting, the third instar larvae of B.

采用在家蚕活体内研究家蚕质型多角体病毒的入侵增殖复制过程，探讨BmCPV的入侵过程和增殖复制机制。

MethodsNeural tubes isolated from the region between midotic placode and third somite of mouse embryo at ED 8.5d were cultured in DMEM/F12 serum-free medium containing basic fibroblast growth factor and epidermal growth factor. The cell cycle of CNCCs was analyzed with flow cytometry, the proliferation capability was determined by MTT method, AP-2α expression was detected by immunocytochemistry as a biological marker of cardiac neural crest cells. The features of migration, multi-directional differentiation were observed as well.

材料与方法成年2月龄C57／BL6小鼠交配，取ED8.5d小鼠胚胎枕中部至第3体节神经管，应用包含碱性纤维生长因子和表皮细胞生长因子的无血清培养基培养，利用MTT法检测细胞增殖能力，利用流式细胞仪检测细胞周期，采用转录激活因子2α(AP-2α，Activator protein-2α)作为其生物学标记物，观察其增殖状况、细胞周期、迁移、分化等生物学特性。

The results were showed that embryogenic calli were induced from young leaves, which were cultured on MS medium supplemented with 2,4-D 2mg/L and KT 0.2mg/L. For the proliferation of embryogenic calli, the suitable culture medium was MS+BA 8mg/L +NAA 1mg/L. The development and maturation of somatic embryo could be much improved by using the medium of MS+ZT 2mg/L or BA 5mg/L +IAA 0.2mg/L. For the induction of secondary somatic embryo from integral somatic embryo, the suitable culture medium was MS+KT 0.1mg/L+NAA 0.01 mg/L or MS+ZT 0.1mg/L+NAA 0.01 mg/L, the proliferation frequency is 214%, 256% respectively. The cotyledonary generated from somatic embryos of Aesculus hippocastanum L.

本文就欧洲七叶树的组织培养和体细胞胚发生以及植株再生进行了系统研究，主要结果如下：以植物细胞具有全能性的理论为依据，以欧洲七叶树幼嫩叶片为外植体，进行体细胞胚胎发生研究，研究结果表明，诱导愈伤组织的适宜培养基是MS+2，4-D 2mg／L+KT0.2mg／L，MS+BA 8mg／L+NAA 1mg／L有利于胚性愈伤组织的诱导和增殖，添加ZT 2mg／L或BA 5mg／L和IAA 0.2mg／L的MS培养基有利于体细胞胚发育和成熟，体细胞胚可直接诱导次生胚发生，MS+KT 0.1mg／L+NAA 0.01 mg／L或MS+ZT 0.1mg／L+NAA 0.01 mg／L培养基诱导效果最好，增殖频率分别为214％、256％。

Las multiplied again in the hemocoel and then invaded other host tissues, including hemocytes, nervous tissue, fat body, trachea, and muscle. Eventually, Las invaded the salivary gland and the Las-infected psyllids were enabled to transmit Las to other host plants. Symptoms of serious pathogenesis occurred in the muscle tissue.

黄龙病菌必需先穿过中肠上皮细胞并於中肠细胞内进行增殖，接著进入血腔继续进行增殖，并逐渐移行至其他组织包括血球、神经、脂肪体、气管上皮细胞及肌肉等，最后抵达唾腺，届时柑橘木虱即具有传播黄龙病的能力。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后，利用SABC法检测CD80的表达；MTT法测定肿瘤细胞体外增殖能力；将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度；在同源淋巴细胞肿瘤细胞混合培养后，通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

Cell immunity and humoral immunity index in group autogenous bone and group xenogenic deproteinizated bone are nonsignificant. BrdU labeled autogeneic MSCs can be detected in xenogenic deproteinizated bone mesh after 4 weeks.

在体支架材料内BrdU标记自体MSCs增殖的大动物示踪研究： XDPB复合BrdU标记的山羊自体MSCs植入术后4周，免疫荧光检测可见BrdU标记细胞在XDPB网孔内生长增殖。

Comparing as BA, 0.1mg·L〓 TDZ was more effective in inducing germinating and developing of buds, regenerating prolific caespitose buds via organogenesis, promoting rhizogenesis and activity of root system greatly, as a result, acclimation survival rate was improved significantly. When leaf segments, cut from in vitro plantlets, were placed on MS media containing 0.1mg·L〓 TDZ, adventitious buds regenerated from segments. After transferred to propagated and rooted media, adventitious buds would proliferate and come into full plantlets.

与BA相比，TDZ的细胞分裂素活性至少高出一个数量级，0.1mg·L〓的TDZ能有效地诱导四个基因型芋茎尖的萌动、生长、大量增殖丛生芽，促进试管苗根系发生，将根系活力提高2倍以上，显著提高了试管苗的驯化成活率；浓度为0.01mg·L〓的TDZ能诱导魁芋类型的芋离体叶片切块产生再生芽点，转接后可增殖不定芽并形成再生植株。

The proliferative capacity of LEC from human had directly relevance to the age of donor(r=-0.996).Conclusions The formation of lens-like spherules is characteristics of LEC lines in the cultured cells.Under identical conditions,the proliferative rate of LEC from bovine and rabbit is fast than that of LEC from human,but dedifferentiation of LEC from bovine and rabbit is easier than that of LEC from human;LEC from the three species exhibit similar limited growth potential.The proliferative rate of LEC from human has a inversely proportion with age.

&晶状体小体&的形成可作为确定晶状体上皮细胞株的一项特征性依据，而体外培养的人、牛、兔晶状体上皮细胞具有相同的有限生长潜能，在相同的条件下，牛、兔晶状体上皮细胞的生长增殖速度比人晶状体上皮细胞快，但易于发生去分化；此外，人晶状体上皮细胞的生长增殖率与年龄密切相关，年龄越小，晶状体上皮细胞的生长增殖速度越快。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。