增殖体

Two specieses, Lycoris radiate and Lycoris aurea, were selected for the studying of artificial propagation in vitro. A few specieses of Lycoris, from the southeast area of China and part of locations in Japan, were chosen to research their karyotype differentiation and to measure their genetic diversity by ISSR analysis. The results could be summarized as follows.1. The vegetative propagation conditions of Lycoris aurea and L. radiate in vitro was studied by two-scaling. Different illuminate condition had an effect on the bulblet formation rate. To the species of L. radiate, the rate was higher in the state of 16h 800-12001x illumination than in the darkness. The medium also affected the new bulblet formating rate. When the culture was MS medium 0.2 mg/L NAA 4 mg/L 6-BA, the bulblet formationg rate of L. aurea was 220%;at the same time, when the medium was MS 0.2 mg/L NAA 2 mg/L 6-BA, the rate of L.

本研究选择西南部分地区及日本的几个石蒜品种，从细胞学和DNA分子角度，分析了它们的遗传多样性水平和遗传结构状况，对红花石蒜和忽地笑的离体繁殖技术进行了初步研究，得出如下结论： 1 进行了红花石蒜和黄花石蒜双鳞片快速繁殖条件的研究，结果表明：红花石蒜在16小时800-1200 lx光照下比黑暗条件下出芽率要高；黄花石蒜在MS NAA0.2mg／L 6-BA4mg／L下出芽率为220％，红花石蒜在MS NAA0.2mg／L 6-BA2mg／L下出芽率为108％；NAA比IBA有利于石蒜生根；硅藻土显著提高黄花石蒜双鳞片出芽率，活性炭起抑制作用；6％蔗糖浓度有利于红花石蒜小鳞茎增重，MS 6-BA4mg／L NAA0.5mg／L培养基有利于小鳞茎增殖，切割一刀比两刀有利于小鳞茎增殖。

Administering adequate but not excessive solution of crystalloid to preserve blood pressure during the recipient surgery.

在反映粘膜上皮细胞增殖程度的DNA含量相对倍体值和细胞增殖核心抗原方面，实验组也明显优于对照组。

Seed explants of Eleutherococcus senticosus produced somatic embryos directly ongrowth regulator-free medium. The results were showed that use low temperature stressmethod to relieve the Eleutherococcus senticosus seed conformation dormancy andphysiological dormancy, to induce seed germinate. Proliferation of Eleutherococcus senticosussomatic embryo without any plant growth regulathors was better in liquid medium containing1/3MS+sucrose 3%, and better in solid medium containing 1/3MS+sucrose 1%.Eleutherococcus senticosus secondary embryo was stick to matrix through espial of cytology.Eleutherococcus senticosus somatic embryo was cultivated some times to form immature frondin liquid medium, then transfer into solid medium to form full plant. The experiment wasindicated that much more Eleutherococcus senticosus immature frond can be cultured inbioreactor, so large-scale production in bioreactor culture can be done.

研究结果表明：低温胁迫处理刺五加种子可以打破种子的形态休眠和生理休眠，促使种子发芽：在不添加任何植物激素的培养基中诱导刺五加体胚形成，其中，1／3MS+3％蔗糖的培养基搭配有利于液体培养的刺五加体胚增值，1／3MS+1.0％蔗糖的培养基搭配有利于固体培养的刺五加体胚增值；在进行体细胞胚增殖时，体细胞胚继代三周后表面开始形成很多次生胚，对刺五加次生胚进行细胞学观察，发现刺五加次生胚与母体只是粘贴在一起；刺五加体胚需在液体培养基中培养一段时间形成幼植物体，实验表明可以通过生物反应器培养大量的刺五加幼植物体，然后转入固体培养基中形成完整植株，因此可以通过生物反应器培养进行规模化生产。

In conclusion, the lag phase of Streptococcus thermophilus was shorten in the eutrophy culture medium, and the time achieving the culminated point of logarithmic phase was shorted also, at the same time, the stationary phase lengthened. After secondary culture, Streptococcus thermophilus reached directly the log phase hardly through the lag phase, and then quickly reached the decline phase. There were more relations of the metabolic regalities of the three kinds of nutritive substances with the super-concentrated incubation process of Streptococcus thermophilus: the more the number of the bacteria, the quicker the metaboling, while the fewer, the slower the metabolism. Moreover, there apparently existed morphologic changes in this course, and there maybe existed the apoptosis, which correlated with bacteria propagation and acid producing. With the culture time postponed, the nutritive substances in the culture medium lacked also, and the morphologic change and apoptosis appeared more obviously.

结果表明，嗜热链球菌在营养丰富的培养基中的生长延滞期缩短，到达对数生长顶点的时间变短，稳定期的时间延长，当经过二次培养后，嗜热链球菌几乎不经过延滞期直接到达对数期，平稳期持续的时间较短，很快到达衰亡期；嗜热链球菌在超浓缩培养过程中三大营养物质的代谢规律也同它的培养过程有很大的关系，菌数多，其代谢旺盛，菌数降低其代谢速度减慢；嗜热链球菌在超浓缩培养过程中存在着明显的菌体形态变化，并可能存在着细胞凋亡，与菌体增殖和产酸规律密切相关，随着培养时间延长，培养基中营养物质变得贫乏，菌体形态变化和细胞凋亡的情况愈加明显。

It is obvious that callus induction and proliferation of gerbera need growth regulators, and are influenced by varieties and explants of different parts of the plants.

以上结果说明，生长素是非洲菊愈伤组织诱导和增殖所必须的，非洲菊愈伤组织诱导、增殖和芽再生方式受品种及外植体类型的影响。

Results:Whith the time and doses of ART incubation extended, ART significantly inhibited the proliferation of SGC-7901 cells and the inhibited effect shows dose-and -time-dependent. Obvious changes of apoptotic morphology were observed by invert microscope, fluorescence microscope, scanning electron microscope and transmission electron microscope, they showed that cells had marked nuclear condensation or the fragmentation of chromation as well as apoptotic , mitochondrial edema and vesicle , with the time of incubation extended , the proliferation rate become slow and the volume became small and transformed. FCM assay indicated that most of the cells were arrested in Go/Gi and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. At the same time, the number of S and G2/M phase cells were decreased. The result of TUNEL indicant that there are apoptosis and necrosis.

结果：随着药物浓度的增加和作用时间的延长，蒿甲醚对胃癌SGC-7901细胞的抑制作用呈时间和浓度依赖关系：在倒置显微镜、荧光显微镜、扫描电镜和透射电镜下可观察到典型的凋亡细胞的形态改变，表现为：核固缩或染色质边聚或凝聚成大块状，可见凋亡小体，线粒体肿胀增殖，严重的空泡化，随作用时间的延长，细胞增殖速度减慢，细胞体积缩小变形；流式细胞术显示胃癌SGC-7901细胞出现明显的凋亡峰，随着作用时间的延长，其凋亡率逐渐升高，细胞周期阻滞在G_0/G_1期，S及G_2/M期细胞数大量减少；流式细胞术TUNEL检测结果显示细胞凋亡和坏死同时存在。

With mature seeds of C. dayanum as explants, different media were selected to establish asymbiotic germination technique system With protocorms as materials, conservation, proliferation resumption and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily. Mature seeds of C.

方法］以冬凤兰种子为外植体，选择不同培养基建立冬凤兰非共生萌发技术；同时利用原球茎为材料，选择低温离体保存，恢复增殖及植株再生条件，初步建立冬凤兰低温离体保存技术体系。

The results indicated that the mutant protein promoted the survival of dorsal root ganglion、induced TF-1 prolification and made the normal mice lose weight,decrease appetite and reduce fat index.The weight loss effect was dependant with its administration dosage,ED50 was 150.986?

结果是突变体蛋白能促进鸡胚背根神经节的生长；促进TF-1细胞增殖，MTT测定法表明突变体蛋白与国际参考品相比，比活不低于2.0×106U/mg；使正常小鼠的体重减轻，摄食量减少，脂肪指数下降，并且体重的减轻与突变体蛋白的给药剂量呈现良好的剂量依赖关系，其ED50为：150.986？

These uncontaminated explants showed that morphogenetic potential were 70%-75% and each responding explant had 1.2-1.4 shoots formation on MS medium supplemented with 2 mg/L NAA and 16 mg/L BA. Moreover, adding antibiotics (100 mg/L Rifampicin and 100 mg/L Gentamycin) to solidify MS contained 3% galactose were tested, results showed the lowest contamination rates were 20% and 1.0-1.2 shoots were regenerated per responding explant. The antibiotic treatments with 100 mg/L Rifampicin and 100 mg/L Gentamycin could reduce internal contaminants, while the addition of antibiotics into medium in vitro culture led to phototoxic phenomena of axillary explants, morphogenetic potential lost and the growth of regenerant was also inhibited.

以100 mg/L Rifampicin+100 mg/L Gentamycin或100 mg/L Rifampicin+100 mg/L Gentamycin+150 mg/L Streptomycin组合浓度处理者，具有显著降低污染率的效果分别为60%及50%；克服内生菌污染而建立初代无菌培养之腋芽培植体，则以100 mg/L Rifampicin或以100 mg/L Rifampicin+100 mg/L Gentamycin处理者，芽体再生率最高70%-75%，惟平均每一腋芽培植体芽体增殖数仅1.2-1.4个；另在醣种类及浓度与抗生素试验，显示100 mg/L Rifampicin+100 mg/L Gentamycin之抗生素浓度组合添加至含3g/L半乳糖的培养基，显著降低污染率至20%；但未污染之培植体，形态发生潜能低至40%以内，可反应生长之培植体芽体形成数也仅达1-1.2个之间，再生芽体之生长也显现抑制现象。

This is resulted from the multiplication of the original nucleates or clusters in the low temperature melt. And the mixing mode of high and low temperature melts play an important role in the MTT process.

从原子团簇的角度分析认为，这是由於高、低温熔体混合后，低温熔体中大的原子团簇得到细化，从而导致混合熔体中形核质点增殖的结果。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。