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The results showed as:(1)Gegen decreases fasting insulin and insulin resistance index and increases insulin sensitive index of rats of insulin resistance induced by dexamethasone significantly;(2)Gegen can improve glucose tolerance of rats of insulin resistance but not to alloxan indued diabetes mice;(3)Gegen increases glucose intake of 3T3-L1 adipocytes and improves the insulin resistance of 3T3-L1 adipocytes induced by dexamethasone significantly;(4)Gegen can activate peroxisome proliferator- activated receptor gamma, make it bind to PPAR response elements;(5)Gegen can up-regulate mRNA expression of PPAR v ,aP2 and adiponectin.

结果表明:①葛根显著降低地塞米松诱导的胰岛素抵抗大鼠血清胰岛素水平和胰岛素抵抗指数,升高胰岛素敏感指数,改善大鼠的胰岛素抵抗;②葛根显著改善胰岛素抵抗大鼠的糖耐量,但对四氧嘧啶糖尿病小鼠的糖耐量没有明显的作用;③葛根显著增加胰岛素抵抗3T3-L1脂肪细胞对葡萄糖的摄取,改善地塞米松诱导的3T3-L1脂肪细胞的胰岛素抵抗;④葛根能促进HepG2肝癌细胞过氧化物酶体增殖物激活受体γ与靶基因的特异DNA序列结合,对PPARγ及其下游基因的转录具有激活作用;⑤葛根能够促进PPARγ及其下游基因脂肪酸结合蛋白(aP2)、脂联素mRNA的表达。

The utilization of the XP1 will contribute the investigation of the role in the process of megakaryocyte differentiating, maturating and platelet formation. Our study would provide additional therapeutic drug as for thrombocytopenic patients.

这一新型的cMp1分子配体将会促进有关巨核细胞增殖和分化及其血小板产生的分子机制的研究,也为开发具有自主知识产权的升高血小板新药打下了基础。

The axillary bud on young shoot of Barbados nut was used as explants,adding different concentration of plant hormone combinations(6-BA and IBA) on minimal medium,to carry out buds inducing and multiplication culture.

以麻疯树幼嫩枝条腋芽为外植体,以M S为基本培养基,附加不同浓度的激素组合(6 BA与IBA)进行芽诱导和增殖培养。

Investigations into PPAR agonists may develop new approaches to the treatment of skin disorders.

过氧化物酶体增殖因子活化受体激动剂的研究可能提供皮肤病治疗的新手段。

Giemsa staining was used to observe the growth of C.andersoni,and transmission electron microscope was used to observe the developmental stages of C.andersoni.MTT assay was used to evaluate the toxicity of nitazoxanid and ginkgoic acids to HCT-8 cells.C.andersoni parasites growing in HCT-8 cells were treated with different concentrations of NTZ and GAs,and the copies of COWP gene were determined by Real-time PCR.

安氏隐孢子虫卵囊感染人结肠癌(HCT-8)细胞,Real-time PCR技术测定RPMI-1640培养液中分别加入不同浓度胎牛血清(fetal calf serum,FCS)、葡萄糖(glucose,GLU)、维生素C(ascorbic acid,ASC)、叶酸(folic acid,FOLIC)、泛酸钙(calcium pantothenate,CAPAN)和胰岛素(insulin,INSULIN)后虫体的增殖效果。

Focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

结果]各项酶学检验无异常;组织学光镜下观察到竖脊肌纤维部分溶解变性,肌纤维肥大,少量炎性细胞浸润;电镜下观察到肌纤维灶状溶解,核周线粒体聚集,胞内脂滴、溶酶体增多,肌卫星细胞增殖分化。

The protection of AA on hepatic GSH level insulted by CTX in mice with Heps solid tumor treated with AA and CTX confirmed the attenuated toxicity effects of AA, which might be associated with hepatopretection of AA.

另外,也发现AA具有抑制肿瘤细胞增殖的作用[9, 10]。但是对于AA应用于在体抗肿瘤治疗的实验研究尚未见报道。

In this study, we amplified both heavy and light genes from anti-SP hybridoma cells, which were later cloned and expressed on the surface of M13 phage.

在本研究中,我们利用PCR的方式来大量增殖重链和轻链的产物,并将其接到质体上,并表现在的M13噬菌体的表面上。

Objective: Cyclooxygenase-2 (COX-2) and peroxisome proliferators-activated receptor-gamma play very important roles in occurrence, progression and prognosis of tumor. But the definite effects and mechanisms of specific COX-2 inhabitor and PPARγagonist on the prevention and cure of pancreatic cancer have not been fully elucidated.

目的:近年来研究表明,环氧化酶-2(cyclooxygenase-2, COX-2)和过氧化物酶体增殖因子活化受体γ在肿瘤发生、发展中起十分重要的作用,但特异性COX-2抑制剂和PPARγ激动剂对胰腺癌的防治作用及其机制尚未十分明了。

AIM:To investigate the effects of pEGFP-TGF-β1 plasmid DNA on proliferation and survival of human lens epithelial cell.

摘要目的:探讨脂质体介导的TGF-β1基因(pEGFP-TGF-β1)转染对人晶状体上皮细胞系B3(HLEC- B3)增殖、凋亡及细胞周期的影响。

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