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The background survey and tracking survey of artificial reef area in Chenghai Laiwu were carried out before and after reefing in 2003 and 2007, respectively. The results indicated that after the deployment of artificial reefs, the biomass density of fishery resources increased by 25.63 times, which were much higher than before. The resources species was much more abundant; total number increased from 23 to 41, which increased by 0.78 times. The crab resources sharply increased by 1.75 times after artificial reef were deployed. Harpodon nehereus and Portunus sanguinolentus , which did not appeared in background survey, now became the dominant species in tracking survey. There were high diversity indexes in artificial reefs area and control area in tracking survey. In conclusion, the results indicated that the fish-aggregating effect of artificial reef site has improved a lot, and the community structure and the habitat environment have been significantly improved.

摘 要:2003和2007年分别对澄海莱芜人工鱼礁区进行了投礁前的本底调查和投礁后的跟踪调查,结果表明,投礁后礁区海域游泳生物的资源密度明显比投礁前高,增加了25.63倍;礁区海域各类资源种类均比投礁前丰富,总种数由投礁前的23种增加至41种,比投礁前增加了0.78倍,其中,蟹类种数增加最多,增加了1.75倍;在本底调查中没有出现的经济种类龙头鱼 Harpodon nehereus 和红星梭子蟹 Portunus sanguinolentus 在跟踪调查中已成为主要优势种;Shannon-Wienver多样性指数在礁区和对比区均比投礁前有所增加

Content of Chla,β-carotene and zeaxanthin of S. platensis grown in Se decreased, violaxathin, fucoxanthin, 19'-hexanoyloxyfucoxanthin and peridinin increased, Chla and Chlb of D. salina also decreased, but carotene increased, all pigments of D. zhanjiangensis increased except violaxanthin and 19'-hexanoyloxyfucoxanthin decreased. Effect of Ge on pigments of microalgaes were different in different algaes: Chla,β-carotene and zeaxanthin of S. platensis decreased, three other carotene increased, in D. salina, violaxanthin and lutein decreased, violaxathin, fucoxanthin and 19'-hexanoyloxyfucoxanthin increased, all pigments of D. zhanjiangensis increased except fucoxanthin and peridinin decreased; Chla,β-carotene and violaxanthin of Chlorella sp increased, but fucoxanthin and peridinin decreased.

暴露于硒中的钝顶螺旋藻(S.platensis)的叶绿素A、β-胡萝卜素和玉米黄素的含量都减少,岩藻黄素、19'-乙酰氧化岩藻黄素、多甲藻素和紫黄素的含量明显增加,盐生杜氏藻的叶绿素类也减少,但类胡萝卜素的含量则增加,湛江叉鞭金藻(D.zhanjiangensis)中除紫黄素和19'-乙酰氧化岩藻黄素有减少外,其它色素都增加;锗对四种微藻光合色素的影响也不尽相同,钝顶螺旋藻(S.platensis)的叶绿素A、β-胡萝卜素及玉米黄素含量减少,岩藻黄素、19'-乙酰氧化岩藻黄素和紫黄素的含量增加,盐生杜氏藻中除紫黄素和叶黄素有增加外,其它色素都减少,湛江又鞭金藻(D.zhanjiangensis)正相反,除岩藻黄素和多甲藻素有减少外,其它色素都增加,小球藻的叶绿素A、β-胡萝卜素和紫黄素增加较大,但岩藻黄素和多甲藻素则减少。

We also found that the content of Na(superscript +), K(superscript +) and proline in leaves varied with different treated days. Under all treatments, the absorption of Na(superscript +) increased and the content of proline in leaves decreased with the prolonging of treated days. The absorption of K(superscript +) measured on the 7th day and the 21st day increased and was restrained on the 14th day, which lead to the decline of K(superscript +)/Na(superscript +) ratio. While the content of praline measured on the 14th day remarkably increased with the increase of salinity. Our experiment indicated the live oak seems to adapt the salt stress by increasing the absorption of K(superscript +) during the earliest and late stages of salt stress and accumulating proline in leaves during the middle stage of salt stress.

同时发现,盐胁迫处理不同时期对叶片脯氨酸含量和Na、K含量的影响各不相同,在处理7d和21d时,叶片对Na和K的吸收量均有增加,使K/Na维持相对稳定的比值,而脯氨酸含量随NaCl质量质量浓度的增加减少;在处理第14d时,对Na吸收增加的同时,抑制了对K的吸收,导致K/Na比值随NaCl质量质量浓度的增加而迅速下降,而此时叶片脯氨酸含量随着盐质量质量浓度的增加而迅速增加,表明在盐胁迫早期和晚期,弗栎可能通过增加对K的吸收以减轻Na离子毒害效应,而在盐胁迫中期,叶片积累脯氨酸是适应盐胁迫的方式之。

By employing such a method, 5 series of elastomers, including natural rubbers, elastomeric polyester fibers and ethylene-octene, ethylene-methyl acrylate as well as SIS triblock copolymers, were investigated with the purpose of studying elongation induced phase structure and mobility changes of the samples. For NR samples, it was demonstrated that there exists a small amount of crystals at RT even for unelongated samples. For both NR and the soft segment of PEE, with the increase of elongation ratio, the elongation-induced crystallinity increases almost linearly in a certain region of R, in accord with the increase of the lamellar thickness of the crystals; The behavior of ethylene copolymers, in terms of elongation induced morphorpholgy changes, are quite different from NR and PEE. The crystals are found to be gradually destroyed with the increase of R, with accord with the decrease of the lamellar thickness; The motional behavior of the soft segment of SIS, changes from "liquid" to "solid", in terms of 13C NMR spin-lattice relaxation.For many of the aformented samples, the dependence of 13C spin-lattice relaxtion time T1 on R are different for different groups, indicating that the changes of mobility at MHz frequency region with elongation are different for different groups.

通过对天然橡胶、聚醚酯嵌段共聚物、乙烯-辛烯共聚物、乙烯-丙烯酸甲酯共聚物、SIS等高分子弹性体样品聚集态结构和分子运动的研究,获得了一些新的实验结果,如:给出了确切的实验证据,证明了室温下未拉伸的天然橡胶样品中存在着微量的结晶;发现随着拉伸比增加,天然橡胶及聚醚酯样品的软段部分会出现拉伸诱导的结晶,且结晶度在一定范围内呈现近似线性的增加,所形成晶片的厚度也随之逐渐增加;而乙烯共聚物随着拉伸比的增加,原有的结晶会被逐渐破坏,晶片厚度逐渐变薄,结晶中的缺陷逐渐增加;随着拉伸比的增加,SIS中PI软段部分的核磁共振弛豫行为会出现从液态到固态的转变等;在对上述多个体系的研究中,还发现不同基团的自旋-晶格弛豫时间随着拉伸比呈现不同的变化趋势,表明不同基团的高频运动随拉伸比增加有不同的变化规律。

The change of average size of products along the radius at the exist of reactor has also been calculated. The calculated results indicate that the residence time is longer, the average size of products is larger, but the distribution of size is narrower, tending to self-preserving distribution; when the concentrations of reactants increase, the average size of products becomes larger, but the distribution of size becomes narrower; the gradients of reactants are greater, the average size is larger; the increasing of reacting temperature will cause the size of product to be larger under same residence time. The average size of product at the exist of reactor varies along the radius, has a maxim value.

模型计算结果表明,产物粒度随停留时间的增加而增大,但粒度分布变窄,趋向自保分布;产物粒度随反应物进料浓度的增加而增大,粒度分布随进料浓度的增大而减小;反应器中反应物浓度梯度较大(相当于化学反应为快反应)时,相同停留时间下所得到的产物平均粒度增加;反应温度的增加,粒子平均粒径增加,粒度分布变窄;反应器出口处粒子平均粒径,随着与对称轴的距离的增加,有一极大值,先增加,后减小。

Reinhardtii; by 13梸20% or 12?20% in S. obliquus; by 35挆81% or 29~79% in C. pyrenoidosa, respectively. On the other hand, specific growth rate in low or high P media, when CO2 concentration increased from 10 to 51 and 99~tM, increased by 13-46% or 10-44% in C. reinhardtii; by 15梸20% or 8-42% in S.

此外,当CO_2从10μM增加到51和99μM时,莱因衣藻的比生长速率在低P条件下增加13~16%,在高P条件下增加10~14%;斜生栅藻的,在低P条件下增加15~20%,在高P条件下增加8~12%;蛋白核小球藻的,在低P条件下增加20~44%,在高P条件下增加26~111%。

The result of simulation analysis shows that the extent of the consequence is increasing by the rising level of atmosphere stability; the consequence extent is rising first and then decreasing along with the increase of RH of the atmosphere but increasing significantly by atmosphere stability; the consequence is decreasing gradually by the aggrandizing of surface roughness.

结果表明:随着大气稳定度等级的增加,LNG泄漏事故后果严重度逐渐增加;随着空气相对湿度的增加,LNG泄漏事故影响后果先增加后减少,这种趋势随大气稳定度等级的增加而越加明显,且事故后果严重度也相应增加;随着地表粗糙度的增加,LNG泄漏事故影响后果基本呈现出逐渐减小的趋势。

From the point of view of structure,bamboo cell wall ratio,amount of bundle per unit area,fraction of bundle sheath,fraction of vessel increase from the root to the top of the bamboo,while fractionof parenchyma.Single bundle sheath area decreases with the increase of height.Fiber length,fiber width and vessel length increase from the root to the top andget theirs maximum value at the length of 17th node to 23rd node and thendecrease.Fiber length and width ratio slightly decreases while vessel widthincreases with the increase of height.

从构造角度来说,竹材胞壁率、单位面积维管束个数、纤维束比量、输导组织比量从基部到到梢部随着高度增加增加,而薄壁组织比量、单个维管束纤维束面积随着高度增加而减小;纤维长度、纤维宽度、导管长度从基部到梢部随着高度的增加增加,在第17竹节到第23竹节达到最大值,而后下降,纤维长宽比随着高度的增加而缓慢下降,导管宽度随着高度增加而一直增加

The results showed:(1)Nitrogen addition changed the soil physical and chemical properties, enhancing the content of NO3—- N available resources in the soil, increasing the plants height and cover, reducing the vegetation light penetration. With increasing of N addition, both the species richness and the diversity decrease sharply (P 0.001).(2)N addition increased the aboveground biomass significantly (P 0.05). With increasing of N addition, the aboveground biomass increased first then decreased, and the grasses biomass increased while the forbs and legumes biomass decreased.(3)There was a significant linear positive relationship between species richness and vegetation light penetration (P 0.05), and also between aboveground biomass and soil NO3—- N content (P 0.05). The relationship between aboveground biomass and species richness was negative upon enhanced N supply.

结果表明:(1)氮素添加提高了土壤中NO3—- N等可利用资源的含量,增加了植物群落植被的盖度,减小了植被的透光率,随着施氮量的增加群落中物种丰富度显著降低(P 0.001);(2)氮素添加显著改变了植物群落地上生产力(P 0.05),随着施氮量的增加地上生产力呈现先增加后降低的变化趋势,各功能群中禾草生物量显著增加,而杂草和豆科生物量随施氮量增加逐渐减少;(3)物种多样性与植被透光率呈线性正相关(P 0.05);地上生产力与土壤NO3—- N含量呈线性正相关(P 0.05);随着施氮量的增加物种丰富度与生产力之间呈负相关关系。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。

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