基质的

Secondly, the thiolation of protein and the coupling of thiol protein with liposomes were studied, and large unilamellar vesicles with highly immunoactivity and stability were acquired.

首先系统研究了混合饱和类脂体系的脂质体的制备和脂质体的性质，然后研究了蛋白质的巯基化和巯基化的蛋白质与脂质体的偶联，从而获得了具有高的免疫活性的、稳定的大单层脂质体。

AMF strains Glomus mosseae-1 and G.mosseae-2 had higher AMF infection rate than other AMF strains, and G.mosseae-2 had the highest Arbuscule percentage in root samples, so G.mosseae-2 can be used as suitable inoculums in tomato organic soil cultivation. Tomato, AMF, Substrate, Seedling stage, Mineral nutrition uptake

为此本试验比较了几种不同丛枝菌根菌种接种剂对基质栽培番茄生长发育和矿质营养吸收的影响，结合对根部菌根侵染率的观察，以筛选适合番茄基质栽培的适宜丛枝菌根菌种。1材料与方法试验于2004年在布达佩斯匈牙利科学院土

Specifically, itcontains 8 chapters.In chapter 1, the formation, structures, properties and the futureprospect of liposome were thoroughly reviewed;In chapter 2, the stibility and permeability of phopholipid -eleostericacid liposome were studied together with the effect of polymerizationof eleostearic acid. This membrane system was very sensitive to 〓,the effect of 〓 was clarified to increase the aggregation/fusion ofliposomes and made the permeability of mixed liposomes much higher;In chapter 3, two polymerizable conjugated diyne bolaamphiphiles were synthesized. They could form very stable mixed liposome, andthe diyne could be polymerized by UV light in bilayer liposomes, as aresult, the stability of mixed liposome against solvent or surfactantafter polymerization were enhanced. In chapter 4, two kinds of amphiphilic amino acids were synthesized andstable liposomes were formed therefrom After the condensationpolymerization of amino acid in bilayer liposomes, stable polypeptide liposomes were obtained, which had lower phase transition temperatureand higher permeability.In chapter 5, four kinds of glycolipids were synthesized and theiraggregation behavior in water was comparied. When incorporated intophospholipid bilayer membranes, they could increase the phase transitiontemperatures and inhibit the aggregation and fusion of mixedliposomesat lower temperature.In chapter 6 and 7, three kinds of steroidal bolaamphiphiles withdifferent chain lengths were synthesized. Incorporation of steroidalmoiety to the center of lipid bilayer membrane obviously increased themobility of lipid membrane and shifted Tc to lower temperature side incomparasion with cholesterol. The bolaamphiphile which was shorter thanthe hosted lipid bilayer membrane thickness influenced the lipid packingmore obviously.

全文共分8章：第一章对脂质体的形成、结构、性质及展望进行了较为详细的文献综述；第二章研究了磷脂-桐酸脂质体的稳定性，通透能力及桐酸的聚合对这些性质的影响；磷脂-桐酸混合脂质体为一类对〓灵敏的脂质体，〓的作用首先是使脂质体集聚然后使脂质体融合，并加速内包荧光物的释放；第三章通过合成两种可聚合共轭双炔双极性双亲分子DDCA，DDOL，研究了共双炔分子在双分子层脂质体膜上的聚合及对脂质体性质的影响，聚合可以提高脂质体相对于溶剂及表面活性剂的稳定性；第四章合成了两类氨基酸为极性基团的双亲分子，它们均可以在超声下形成稳定的脂质体结构；氨基酸基团可以在脂质体上进行缩聚反应，若聚合后脂质体表面仍有足够的亲水能力，则可得到稳定的多肽型脂质体；聚合后脂质体的相变温度降低，通透能力增加；第五章合成了四种亲水基团为单糖基的双亲分子GL-l，GL-2，GL-3， GL-4，研究了它们在水中的分散情况、集合体形态与分子结构的关系；在DMPC双分子层膜中加入糖脂分子可以使脂质体的相变温度提高，阻止脂质体在低温放置时的集聚与融合；第六章-第七章合成了三种不同碳链长度的双极性含胆甾环双亲分子 CL-1，CL-2，CL-3；它们可以象胆固醇一样与磷脂混合形成稳定脂质体，胆甾环基团位于脂质体双分子层膜的中间；与胆固醇的作用相反，它们可以增加磷脂双分子层膜的流动性，降低混合脂质体的相变温度；三种分子的作用与其碳链长度和磷脂双分子层膜的厚度有关，比膜厚度短的分子影响最大。

Results Among the three groups,the children's rib cartilage had the most blood vessels,the most chondrocytes,well-distributed stain of matrixes,and the type Ⅱ collagen was expressed actively and highest in photedensity.The rib cartilage of teenager group had less blood vessels,unhomogeny distributed stain of matrixes,the enlarged and separated cartilage lacunas.The rib cartilage in adult group showed the least blood vessels,the least chondrocytes.the hyalinization of perichondium,the most deposition of calcium salt,and the type II collagen was expressed at the lowest level in photodensity.

结果 儿童组肋软骨膜血管最丰富，软骨基质染色均匀，软骨细胞数目最多，Ⅱ型胶原蛋白表达最活跃，平均积分光密度值最高；青少年组软骨膜内血管减少，软骨基质染色出现明显的不均质状，软骨陷窝体积变大，并呈分隔状，陷窝内软骨细胞数目减少，II型胶原蛋白表达较儿童组减弱；成人组软骨膜血管、细胞成分明显减少，软骨膜内的纤维成分明显玻璃样变，钙盐沉积较青少年组时明显增多，Ⅱ型胶原蛋白表达较青少年组减弱。

This experiment further investigated the possibility of differentiation of BMSCs in rats into neural stem cells in vitro, and into neural cells and neuroepithelial cells, so as to provide seed cells for cell transplantation of nervous system disease.

目的：已证实骨髓基质细胞可分化为中胚层组织细胞，实验予进一步探讨体外分离培养的骨髓基质细胞向神经干细胞分化的可能性，以及是否能继续定向分化为神经细胞及神经胶质细胞，为神经系统疾病细胞移植治疗提供种子细胞。

This experiment is sought to study the contribution of different gene in osteogenous differentiation of bone marrow stromal cells and optimize the seed cells of bone tissue engineering.

为了优化骨组织工程种子细胞，探讨不同基因对骨髓基质干细胞成骨活性的影响，我们首先利用RT-PCR方法获得了全长BM P-2，VEGF 165及bFGF基因，克隆入真核表达载体pcDNA 3.0，鉴定正确后，经脂质体介导转入分离培养的大鼠骨髓基质干细胞，G 418筛选出稳定表达株。

Two dominate variation trends are distinguished in the data plots of compressional-wave velocity versus Possion ratio, indicating the effects of mud content and calcite cement on the elastic properties of sandstone samples. In each change trend, with the rock texture transfering gradually from grain-supporting to matrix-supporting with increasing mud and calcite cement content, the propagation of elastic wave also changed from grain-to-grain contacts to rock matrix, which resulted in the measured data illustrating extremely different tendency. It helps utilize the physics properties of reservoir sandstone to extract rock texture and diagenesis properties in the research area.

样品在纵波速度－泊松比交汇图中表现出两种主要变化趋势，分别对应泥质与钙质胶结物的影响；在每个变化趋势中随泥质含量与钙质胶结物含量的增加，岩石结构从颗粒所构成的骨架支撑逐渐表现为基质支撑，弹性波能量的传播也从通过颗粒接触边界转变为通过岩石基质传播，这是使砂岩样品测试结果表现出两种截然不同变化趋势的主要原因，为利用岩石物理特性从地震资料中提取岩石结构、沉积与成岩特征的信息提供了依据。

Ethods Tissue specimens were stained immunohistochemically for MMP-2 and MMP-9 in 30 cases of squamous cell cervical carcinoma,29 cases of cervical intraepithelial neoplasia and 16 cases of normal cervices.

MP- 2鳞癌组与CIN组及正常组相比差异有极显著性(P 。0 1)。 MMP- 9正常组与鳞癌组及 CIN组相比差异有显著性(P 。0 5 )。MMP- 2在各组间质的基质细胞中表达均为阳性。MMP- 9在正常宫颈组织、CIN及鳞癌的基质细胞中阳性表达分别为 0 / 16 (0 %)；4/ 2 9(13.8%)；7/ 30 (2 3.3%)。各组间相比差异无显著性。

The final coated particle exiting from the condenser is then introduced into the aerodynamic particle sizer spectrometer or home-built aerosol laser time-of-flight mass spectrometer, and its aerodynamic size directly effects on the matrix to analyte molar ratio, which is very important for MALDI technique.

利用自行研制的蒸发/冷凝实验装置作为基质辅助激光解吸/电离飞行时间质谱仪的特种接口，通过冷凝方式在线添加基质，冷凝管出口处的最终粒子直接引入空气动力学粒径分析仪或自行研制的飞行时间质谱仪，可对实验室产生的生物气溶胶粒子进行实时检测。

The protein matrix in the soft wheat lacks continuity and the structure appears more open.

软质小麦中的蛋白质基质缺乏连续性，因而基质结构显得较松疏。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。