基质的

The suspension cells of the heart at E11 were respectively cultured in co-culture system based in the stromal cells from the heart, the fetal liver and AGM region with the same conceptus age, and c-kit+ cells were analyzed by flow cytometer, estimating the effect of different stromal cells on HSCs\' amplification.

将培养24h后的E11心脏悬浮细胞分别置入由同期的心脏内皮细胞、胎肝基质细胞和AGM区基质细胞构成的共培养体系中，于0h， 24h和48h分别检测c-kit+细胞比率，研究各组织基质细胞对HSCs扩增的影响。

Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A (0.1,1.0,10 mg·L-1),cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase was measured by enzyme linked immunosorbent assay.

含兔角膜基质细胞（1×105·mL-1）三维胶原凝胶表面添加提纯的不同浓度绿脓杆菌外毒素A（0.1、1.0和10 mg·L-1）后，放入无血清MEM培养液中37℃培养24 h，采用光学显微镜观察培养的兔角膜基质细胞形态学变化，采用酶联免疫吸附法检测损伤的兔角膜基质细胞释放的乳酸脱氢酶含量。

This investigation include two parts: lThe primary culture, chondroblast induction and evaluation of MSC. 2The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan.

第一部分人骨髓基质干细胞的原代分离、体外培养、成软骨诱导及鉴定目的：了解骨髓基质干细胞原代培养的特性及增殖能力，探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用；为软骨组织工程提供足量、合适的种子细胞。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Transplantation of BMSCs in neonate rats at 24 hours after HIBD has the maximal long-lasting ethological improvement, which is induced by brain damage, and the mechanism may be that early transplantation with BMSCs reduces effectively neuron necrosis and apoptosis, and is helpful for the migration and differentiation of BMSCs.

新生大鼠缺氧缺血性脑损伤后24 h接受骨髓基质细胞移植可最大程度改善脑损伤所导致的远期行为学障碍，其机制可能与早期移植可有效减轻神经元的坏死、凋亡，且有利于骨髓基质细胞的迁移、分化有关。关键词：骨髓基质细胞；细胞移植；缺氧；缺血；脑；新生大鼠

GRP can also make mice's MNC and hematopoietic stem cells from human's umbilical cord blood amplification. We use colony formation to analysis the linear relation during the cells'growth and the medicine's dosage, examine the amplification of CFU-GM induced by hypothallus and its supernatant, RT-PCR is used again to check three cytokines'genes.

发现GRP对小鼠MNC扩增有作用，从而将GRP运用到人脐带血MNC的扩增分化上，用集落形成法分析药物与扩增的线性关系，基质层、基质上清对CFU-GM的影响，用RT-PCR技术检测基质层细胞生长因子基因的扩增。

This review focused on the biological significance of matrix metalloproteinases in its regulation when mesenchymal stem cells differentiate into myoblastic, osteoblastic, chondroblastic, neural, and apidoblastic lineages.

文章详细阐述了基质金属蛋白酶在间充质干细胞分化为成肌细胞、成骨细胞、破骨细胞、神经细胞、脂肪细胞过程中所起的重要生物学作用，并对基质金属蛋白酶在间充质干细胞分化中的调控作用进行分析。

Ovarian stromal flow PSV, EDV and perifollicular vascularity are highly interrelated with ovarian response. Ovarian response will be improved when ovarian blood velocity increases and perifollicual blood flow is abundant. Follicular fluid VEGF level was obviously higher in poor responder group, which suggests VEGF be secreted increasing for compensating due to reduced ovarian stromal blood velocity and fewer perifollicular vascularity.

卵巢基质内血流的PSV、EDV和卵泡周血流分布、卵泡液VEGF水平与卵巢的反应性密切相关，卵巢基质内血流速度升高、卵泡周边血流丰富，有助于提高卵巢的反应性；低反应卵巢组卵泡液VEGF水平显著升高，推测是由于其卵巢基质内血流速度低，卵泡周血管分布少，导致VEGF代偿性分泌增加。

We study the express of heparinase,matrix metalloproteinase MMP-2,MMP-14 and cadherin in the gastric cancer tissue and perigastric normal mucosal tissue,and make a statistics analysis between heparinase, matrix metalloproteinase MMP-2,MMP-14,E-cadherin and gastric cancer biological behavior.We hope to knom the possibility that heparinase,matrix metalloproteinase MMP-2,MMP-14,E-cadherin are regared as clinical screen index of the gastric cancer recrudescence or metastasis.

本文就肝素酶、基质金属蛋白酶MMP-2、MMP-14、钙粘蛋白在胃癌组织及胃周正常粘膜的表达进行了研究，并就肝素酶、基质金属蛋白酶MMP-2、MMP-14、钙粘蛋白与胃癌的生物学行为进行了统计学分析，以期了解肝素酶、基质金属蛋白酶MMP-2、MMP-14、钙粘蛋白作为胃癌复发或转移的临床筛选指标的可能性。

Objectives: To study the relationship between matrix metalloproteinases and their inhibitors and the invasion and metastasis of human glioma, as well as the relationship between the expression of MMP-2 and TIMP-2 in human primary and recrudescent glioma and the grade of malignancy.

目的：探讨基质金属蛋白酶类与胶质细胞瘤浸润性生长之间的关系及其在胶质瘤复发中的作用，以及基质金属蛋白酶及其抑制因子(MMP-2、TIMP-2)的阳性表达与胶质细胞瘤病理分级及预后的关系。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。