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In view of practical problem during Sulige gas field development, this paper complete follow study content. Study material balance theory of two district compound gas reservoir. Carry out stress sensitivity experiment on matrix rock and labor created fracture core from Sulige gas field with America Core Company high pressure high temperature flow experimental instrument to study stress sensitivity change rule of different permeability matrix rock and created fracture core at the condition of time and again switch on and off well and different drawdown pressure. Carry out depletion experiment on matrix rock of different permeability serial combination with different kinds of allocation, using double core clamper serial connected experimental apparatus, to study gas well production performance of bi-zonal compound low permeability reservoir during low pressure production stage. At the basis of experimental study, establish single well simulation model to analyze development index infection caused by stress sensibility and production performance infection rule caused by inner formation and outer formation of bi-zonal compound gas reservoir permeability variation while considering stress sensitivity.

针对苏里格低渗气田开发中存在的实际问题,完成以下研究内容;研究了两区复合气藏物质平衡原理;利用美国岩芯公司高温高压流动实验仪,对取自苏里格气田基质岩芯和人工造缝岩芯进行应力敏感实验,研究不同渗透率基质岩芯和造缝岩芯在多次开关井和不同生产压差条件下的应力敏感变化规律;采用双岩芯夹持器串联实验装置,对不同渗透率串联组合的基质岩芯进行各种配产条件下的衰竭实验,研究两区复合的低渗储层气井在低压生产阶段的生产动态特征;在实验研究的基础上,建立单井模拟模型分析储层应力敏感性和非均质性对开发指标的影响,研究考虑应力敏感时两区复合气藏储层中内区和外区渗透率变化对开发动态的影响规律。

It was shown that the matrix suction decreased with the increase of the water content and density. The relationship among temperature, density, matrix suction and water content was exponential.

试验数据分析表明:基质吸力随含水率的增大而减小;基质吸力随密度的增大而增大:温度、密度、基质吸力与含水率之间符合幂函数的变化关系。

Based on the evaluation system, the plants whose relative remediation age was between 0~10 years were named as"Hyperaccumulator"and those between 10 ~ 50 years were named as"Accumulator".7 In the experiments six kinds of substrates were mixed with different solid wastes and the absorption and enrichment of plants on heavy metal Cd was studied. The results showed that relative remediation age was an ideal index to evaluate the capability of the phytoremediation on heavy metals, because it took into account more factors which affected the absorption evaluation effects than others, such as the heavy metal content and the biomass of the over-ground part of plants, the heavy metal content and the whole gross in substrates, harvesting frequency of plants and the environment standard of heavy metal. This made relative remediation age be suitable to the sorption capability evaluation of not only different plants on one heavy metal but also one plant on different heavy metals.8 It can be drawn from the relative remediation age of heavy metal Cd that the absorption capability of different plants was not same.

以相对修复年限为评价指标定义相对修复年限为0-10年的植物为"超富集植物",10-50年的植物为"富集植物"。7在由不同废弃物按一定的比例混合配制的六种土地复垦基质上,系统地研究了几种植物对重金属镉的吸收和富集情况,结果表明,相对修复年限既适用于不同植物对同一种重金属元素的吸收能力的评价,又适用于同一植物对不同重金属元素的吸收能力的评价,是衡量重金属元素植物修复效果的理想指标。8从不同植物对同一基质重金属镉的吸收和富集能力看,不论是经过多年种植的基质,还是第一次种植的基质,小白菜和油菜都是相对理想的植物;而黑麦草、高羊茅和苜蓿的吸收效果并不理想。

Methods Porcine bile duct and ureter were treated using detergent sodium dodecylsulphate and 1% Triton X-100 to prepare the acellular lumens matrix. The toxic effects of different concentrations of acellular lumens matrix extract were tested by MTT to assess the proliferation of human scarfskin fibroblasts. The cytotoxicity of the target biomaterial was graded according to the national standards. The growth manner of the human intrahepatic bile duct endothelial cells seeded on the acellular lumens matrix was studied after 20 d under scanning electron microscopy.

采用十二烷基磺酸钠、Triton X-100处理封闭群小香猪输尿管和胆管,获得去细胞管腔基质支架材料; MTT法检测基质支架材料的不同浓度浸提液对细胞增殖的影响,并按医用生物材料细胞毒性分级标准进行评级;在基质支架材料上种植人肝内胆管上皮细胞,于接种后20 d通过扫描电镜观察人肝内胆管上皮细胞在支架上的生长情况。

Methods A lamellar flap was produced in the rabbit cornea with a microkeratome. The flap interface in whole corneas and corneal beds were soaked in the rAAV –EGFP solution for 10 min, BSS solution was applied in the control group. The corneas were harvested at day 1, 3, 14, and 21 after application for the structure analysis of cornea by HE staining. Expression of the transferred gene was monitored by fluorescence stereoscope and laser confocal microscope. Results After 3d of infection with rAAV-EGFP ,slight fluorescence was detected in rabbit cornea by fluorescence stereoscope .

微型角膜刀制作兔角膜基质瓣;转染组用含rAAV-EGFP转染液浸泡角膜瓣和角膜基质床10 min,对照组用等量BSS液浸泡角膜瓣和角膜基质床10 min,不同时间点(1、3、7、14、21d)通过荧光体视镜观察角膜出现EGFP荧光的起始时间及分布情况,收集角膜,其中一半用激光共聚焦显微镜观察、照相,另一半固定后行HE染色。

Based on the evaluation system, the plants whose relative remediation age was between 0~10 years were named as"Hyperaccumulator"and those between 10 ~ 50 years were named as"Accumulator".7 In the experiments six kinds of substrates were mixed with different solid wastes and the absorption and enrichment of plants on heavy metal Cd was studied. The results showed that relative remediation age was an ideal index to evaluate the capability of the phytoremediation on heavy metals, because it took into account more factors which affected the absorption evaluation effects than others, such as the heavy metal content and the biomass of the over-ground part of plants, the heavy metal content and the whole gross in substrates, harvesting frequency of plants and the environment standard of heavy metal. This made relative remediation age be suitable to the sorption capability evaluation of not only different plants on one heavy metal but also one plant on different heavy metals.8 It can be drawn from the relative remediation age of heavy metal Cd that the absorption capability of different plants was not same.

以相对修复年限为评价指标定义相对修复年限为0-10年的植物为&超富集植物&,10-50年的植物为&富集植物&。7在由不同废弃物按一定的比例混合配制的六种土地复垦基质上,系统地研究了几种植物对重金属镉的吸收和富集情况,结果表明,相对修复年限既适用于不同植物对同一种重金属元素的吸收能力的评价,又适用于同一植物对不同重金属元素的吸收能力的评价,是衡量重金属元素植物修复效果的理想指标。8从不同植物对同一基质重金属镉的吸收和富集能力看,不论是经过多年种植的基质,还是第一次种植的基质,小白菜和油菜都是相对理想的植物;而黑麦草、高羊茅和苜蓿的吸收效果并不理想。

Specific inhibition of Hh signalling using small molecule inhibitors, a neutralizing anti-Hh antibody or genetic deletion of smoothened in the mouse stroma results in growth inhibition in xenograft tumour models.

由癌细胞分泌的Hedgehog配体,在肿瘤表皮细胞中未能激活信号作用,而是在&基质&上发挥作用(&基质&由细胞外基质、成纤维细胞、内皮细胞和微血管组成,恶性细胞着床于其中)。

Method] 5 matrices: M1(50% peat + 50% perlite),M2(40% peat + 60% perlite),M3(50% peat + 50% chestnut soil),M4(40% peat + 60% chestnut soil) and M5(100% peat) used for Euonymus fortunei cutting in container were tested and the effect of the different matrices on the rate of callus induction and root growth,the length and number of root,and the growth amount of Euonymus fortunei was investigated.[Results] The results showed that the best effect on Euonymus fortunei cutting was from the M1,in which,the rate of root growth,callus induction,the length of root and the number of new branch were 87%,88%,68.0 mm and 4.6 branches,respectively.

方法] 采用5种基质配比,分别为M1(50%泥炭+50%珍珠岩)、M2(40%泥炭+60%珍珠岩)、M3(50%泥炭+50%栗钙土)、M4(40%泥炭+60%栗钙土)、M5(100%泥炭)对扶芳藤进行容器扦插育苗试验,对不同基质引起的扶芳藤愈伤率、生根率、根长、单株根系数目、新生枝数及新生枝生长量进行调查分析,[结果]结果表明,基质M1中扦插的扶芳藤生根率、愈伤率最高,分别为87%和88%;根长最长,为68.0 mm;平均新生枝数最多,为4.6条,效果最佳。

Six soil free mixtures (Ⅰ. decayed grass fibre : slag : chicken dung = 3 : 1:1;Ⅱ. Decayed grass fibre: peat: slag2 : 2 :1;Ⅲ Decayed wood fibre: slag: chicken dung3 : 1: 1;Ⅳ. Decayed wood fibre: peat: slag2 : 2 : 1;Ⅴ. peat : slag chicken dung=2 : 1 : 1;Ⅵ. Peat : vermiculite : perlite = 2 : 2 : 1;Ⅶ. Soil.) were used as growth medium for perennial ryegrass turf establishing experiment.

以单一的基质为材料,调制成6种混合无土基质,分别为:Ⅰ3腐熟草质纤维十炉渣+鸡粪;Ⅱ2腐熟草质纤维+2泥炭十炉渣;Ⅲ3腐熟木质纤维+炉渣+鸡类;Ⅳ2腐熟木质纤维+2泥炭+炉渣;Ⅴ2泥炭十炉渣+鸡类;Ⅵ2泥炭+2蛭石+珍珠岩、Ⅶ土壤。

Method]Isolated bMSCs were cultured in vitro. rh Fibroblast Growth Factor 1(rhFGF-1)、rh Transforming Growth Factor-β1(rhTGF-β1)、rh Insulinlike Growth Factor-Ⅰ were utilizated. The proliferation of cells was detected by MTT assay, and the macroscopic histology , HE staining and immunohistochemical examinations were performed to seek the best cell factor. In vivo , to investigate the repair of the articular cartilage, bMSCs combined with fibrin glue and rhTGF-β1、rhIGF-I was compared with control group.

方法]rhFGF1、rhTGF-β1、rhIGF-I单独或联合应用对骨髓基质干细胞进行体外诱导培养,应用常规染色、MTT、免疫组织化学染色的方法筛选诱导骨髓基质干细胞成软骨细胞的最佳细胞因子,并将其与骨髓基质干细胞复合于纤维蛋白凝胶制成凝胶复合物,直接种植到兔膝关节实验性关节软骨缺损处,并与对照组相比较,观察软骨修复效果。

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