基质外的

CD44 is a single strand glucoprotein family encoded by single gene on the memebrane surface with high heterology,which can mediate adhesion between cell and cell as well as cell and extracellular matrix.Its different splicing variants will result in the variety of its gene products.Especially,its splicing varant V6 (CD44v6) is condided to have oncogenicity.CD44v6 takes part in the genesis and development of tumor at some certain and is one of prognostic factors.So it will be the marker indicating tumor development.

CD44是由单一基因所编码的具有高度异质性的单链膜表面糖蛋白家族，介导细胞与细胞、细胞与细胞外基质之间的黏附作用，其拼接变异体的不同构成了其基因产物的多样性；尤其拼接变异体V6(CD44v6)被认为具有致瘤性；CD44v6在一定程度上参与了肿瘤的发生发展，是预后因子之一，并且可能作为肿瘤进展的标志物。

FN and LN (the non-collagenous index) out of ECM were measured by immuno-histochemistry mean, and the accumulation of ECM was measured by typeⅠand Ⅲ collagen ,α-SMA was chosen as a symbol of the activated reposit fat cell. TGF-β〓 was chosen as a symbol of the cellular factor made the reposit fat cell activated. We observed the mechanism of 286 through the variety of the upper indexes during the experiment.

用免疫组化方法测评肝细胞外基质中的非胶原指标FN、LN，胶原指标Ⅰ、Ⅲ型胶原等在实验过程中的变化作为判定ECM沉积增多的事实指标；用a-平滑肌肌动蛋白作为贮脂细胞被激活的标志物指标；用转化生长因子-β〓作为激活贮脂细胞的细胞因子指标；通过观测上述指标在实验中的变化，来探讨"268方"的作用机理。

FN and LN(the non-collagenous index) out of ECM were measured by immuno-histochemistry mean, and the accumulation of ECM was measured by type I amd III coHagen, a -SMA was chosen as a symbol ef .the activated reposit fat cell.TGF- P1 was chosen as a symbol of th ieccellular factor made the reposit fat cell activated.We observed the mi sclnanism of 286 through the variety of the upper indexes during the experiment.

用免疫组化方法测评肝细胞外基质中的非胶原指标FN、LN，胶原指标Ⅰ、Ⅲ型胶原等在实验过程中的变化作为判定ECM沉积增多的事实指标；用a-平滑肌肌动蛋白作为贮脂细胞被激活的标志物指标；用转化生长因子-β_1(TGF-β_1)作为激活贮脂细胞的细胞因子指标；通过观测上述指标在实验中的变化，来探讨"268方"的作用机理。

ConclusionsRupture or disspearence of internal elastic layer, soakage of inflammation cell, different stage of atherosclerosis, apoptosis of SMC and changes of ECM, and so on are the pathological and morphological base of the occurrence and development of ISA.

内弹力层的断裂或消失、炎性细胞浸润、动脉粥样硬化改变、中膜SMC的凋亡变薄、细胞外基质纤维的排列紊乱、减少等变化是ISA发生发展的病理形态学基础。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

RESULTS: The extracellular proteoglycan can be detected as early as 3 weeks and the content increased over time.

结果：在微球培养的第3周就可以检测到细胞外基质中的蛋白聚糖，并且随着时间的增长其单位细胞的蛋白聚糖分泌量不断增加。

To address more specifically the mechanisms involved in cortical bone healing, we created drill-hole defects in the cortex of mouse femur, a lesion that triggers intramembranous repair, and compared the roles of bone sialoprotein and osteopontin, two proteins of the extracellular matrix, in the repair process.

为了更具体的了解皮质骨修复过程的机制，我们在小鼠股骨上制造了一个钻孔缺损，这一损伤能引发股骨的膜内修复，并比较细胞外基质中的两种蛋白骨涎蛋白和骨桥蛋白在这一修复过程中的作用。

The highly specialized lamellar organization of the anulus is well recognized and suggests that this structure may be the result

目前我们所知的纤维环高度分化的板层结构提示，这种结构可能就是极化的间盘细胞定向分泌细胞外基质形成的。

Therefore, collagen metabolism is the center of the extracellular matrix remodeling in intracranial saccular aneurysms, while the essence of the remodeling process is a kind of autoimmunological and inflammatory reaction.

因此，胶原代谢是囊性动脉瘤细胞外基质重塑的核心内容，而重塑过程的实质是一种以细胞免疫为主的免疫炎性反应。

And also the relationship of the expressions of TN and survivin in breast cancer was assessed.

1。随着乳腺组织从正常到癌变的过程中TN在细胞外基质中的表达逐渐降低，而在上皮细胞中的表达则成上升趋势。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。