基膜

Under normal temperature.the Ca2+-ATPase activity was found on the innerside of the PM.on the vacuole envelope and the chloroplast envelope,and theenzyme activity declined upon heat stress.The Ca2+-ATPase activity on the vacuoleenvelope,PM.and the chloroplast envelope in both Ca2+-and oxalate-treated peppermesophyll cells was higher than that in the control under the same condition.TheCa2+-ATPase was also found at the granum lamella,and it was activatedsignificantly by exogenous Ca2+ and oxalate treatments.Under heat stress.the Ca2+-ATPase activity declined slowly in both Ca2+- and oxalate-treated mesophyll cells.But La3+ treatment inhibited the enzyme activity under both normal condition andheat stress.

常温下生长的叶肉细胞，在质膜、液泡膜、叶绿体被膜等处有Ca2+-ATPase活性，热胁迫后酶活性下降；外源Ca2+和草酸预处理对辣椒叶肉细胞各种膜上Ca2+-ATPase活性具有促进作用，特别是定位于液泡膜、质膜和叶绿体被膜上的酶活性明显比对照提高；在叶绿体基粒和基质片层上也有酶活性，并且Ca2+和草酸预处理对该部位上的酶活性激活作用更明显；La3+处理的作用与Ca2+和草酸处理的效果相反。

High energy Ar ion bombardment is helpful to reduce chlorine at the interface, as well as increasing coating density and enhancing interdiffusion between coating and substrate.

提出了一个提高膜基体系性能的新思路，结果表明，界面处的氩离子轰击有助于减少膜基界面的残余氯含量，提高膜层致密度，增强膜基原子的扩散。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

The results show that the porosity of the membrane prepared by ScCO_2 induced phase separation is higher than that prepared by conventional method;the membrane structures are significantly influenced by the process parameters;compared with the conventional immersion precipitation phase inversion,ScCO_2 induced phase separation process can effectively avoid the formation of macrovoids at suitable operating conditions;during depressurization process,the great differences in the effect of depressurization time on membranes structure for various polymer materials result from that the speed of CO_2 gaseous nuclei escaping from membrane substrate can not keep the pace with the outer depressurization rate.(2) The tendency that the interaction parameters affect the binodal curve position is illustrated by changing the interaction parameters in reasonable ranges.

研究结果表明：与传统方法相比，采用ScCO_2诱导相转化法可获得更高的膜孔隙率；通过改变操作参数可对膜的结构进行调控，且操作参数对膜结构的影响与铸膜体系的自身性质也紧密相关；在适宜的操作条件下，ScCO_2诱导相转化法能有效避免采用传统的浸入沉淀相转化法制膜时易出现的大空腔结构；泄压过程中CO_2气核从聚合物膜基体中逃逸的速度能否与膜基体外部的泄压速度保持同步，是泄压时间对不同材质膜结构影响截然不同的根源所在。

The UV and IR spectroscopic studies of the monomer films showed that the photoreaction ratio of monomer (about 50%) was higher than the corresponding photosensitive polymer (about 10%, PVCi). The polarized IR spectra proved that obvious anisotropic absorption was displayed in the irradiated photosensitive film. With polarizing microscopy method, we found that these kinds of photoalignment films exhibited good alignment ability, and the stability of the photoalignment film could reach about 100℃. 2 A photosensitive self-assembled monolayer was fabricated that contained photo-cycloadditable groups, such as, coumarin and cinnamate. The reaction process were detected by contact angle method because the IR absorption of monolayer was very weak. The photosensitive self-assembled monolayer promote uniform alignment of nematic liquid crystals by LPUV, and it thermal stability could reach about 130℃. 3 A cationic bolaform amphiphiles was synthesized that had two photosensitive double bonds, and can be polymerized by ultraviolet. A layer-by-layer self-assembled deposition film was prepared in aqueous solution by using this material and poly (sodium 4-styrenesulfonate). The UV-Vis. spectra proved that the multilayers film was uniform and layer-by-layer. When LPUV irradiated the film, the photopolymerizable double bond underwent [2+2] cycloaddition, and the production of the reaction distributed along the polarized direction of incident LPUV. Polarized UV-Vis. spectra indicated that the irradiated self-assembled multiplayer film was anisotropic, and the dichromic ration is about 0.09, it is higher than the spin-coating method of photosensitive polymer or monomer. We used this anisotropic film as alignment layer of nematic liquid crystals, and obtained a uniform and stability alignment result, the static contrast ratio is about 100, which is very close to application of liquid crystal device. This thesis is an instance that the photochemical material was used in the physics region of liquid crystals, the route that was from photosensitive monomer, self-assembled monolayer film to self-assembled multiplayer film was undergone, by this logical research method photoailgnment of liquid crystal was extended and consummated.

围绕上述思路开展了如下工作：1合成了三种可进行光二聚反应的光敏小分子单体，LPUV 辐照制作了光控取向膜；用紫外-可见光谱法和红外光谱法分析了薄膜中的光化学反应，发现小分子的光反应程度可达到50%以上，至少比光敏高分子高出5倍；薄膜的偏振红外光谱证实，经过LPUV 辐照后薄膜出现了明显的各向异性；将小分子光控取向膜制成液晶器件，在偏光显微镜下观察看到均匀一致的液晶取向；并且热稳定性达到100℃左右，接近实用化要求。2制备了含肉桂酸、香豆素基团的自组装单层膜；采用接触角的方法监测自组装反应的动力学过程；紫外-可见光谱证实了自组装单层膜在石英基板表面形成；自组装单层膜经LPUV 辐照后能诱导液晶均匀取向，而且热稳定性可达130℃，热稳定性进一步提高。3合成了含有光敏双键的双季铵盐，在水溶液状态下将它和聚乙烯基苯磺酸钠沉积生成layer-by-layer 自组装多层膜，紫外-可见光谱证实这是一个逐层均匀的沉积；在LPUV 照射下，自组装膜中的光敏双键按照偏振方向发生[2+2]环加成反应，形成取向膜，用二向色性值评价的分子取向度高于高分子及小分子光控取向膜的水平；实验证明用自组装多层膜制成的液晶器件亮暗态均匀，静态对比度约为100，达到实用化器件的水平。

The studies of hydrogen behaviour in HR-1 stainless steel and its composite sample with CVD coated chromium oxidic film have been presented.

本文介绍氢在HR－1不锈钢基体及镀有氧化铬膜的复合材料中的行为研究，包括基体的准备；膜的制备；用不同手段从不同角度对膜进行的分析测试；膜与基体之间的平均结合力测定；用不锈钢材料构成的高温低压渗透回路的建造、调试、密封性能校准；纯HR－1基体材料中的氢渗透规律研究；有膜复合材料中氢的稳态迁移过程的表现规律实验结果。

Results: Before the fixed bridge was applied, under vertical load, the high-stress region was near around the apical of periodontal ligament with mainly compressive stresses. Under oblique load, the high-stress region was near around the cervical of periodontal ligament with both tensile and compressive stresses. After the fixed prosthodontics, stress and strain distributions were similar with that when the fixed bridge was not applied. But the maximum stress, strain and displacement increased apparently. The stresses in exterior surface of periodontal ligament were larger than that of interior surface; this character of periodontal ligament under vertical load was more obvious than that under oblique load.

结果：固定桥修复前，垂直载荷下，基牙牙周膜的应力以压应力为主，应力集中在根尖；斜向载荷下，基牙牙周膜的应力主要是拉应力和压应力，应力集中在唇、舌颈缘；固定桥修复后，基牙牙周膜的应力和应变分布规律与修复前相似，但最大应力、应变值都明显增大；牙周膜内表面的应力大于牙周膜外表面；垂直载荷时，牙周膜内、外表面的应力差异更为显著。

Two series of polyurethane acrylate grafted acrylic resins (Acr-g-TPU-PETA and Acr-gPPU-PETA) self-emulsifying systems are obtained by grafting -NCO group modified polyurethane acrylates with acrylic copolymer prepared from n-butyl acrylate, styrene,βhydroxyethyl methacrylate and acrylic acid The self-emulsifying systems showed Newtonian fluidity with viscosity closed to that of water, the solid contents were around 30% The T〓 of 15～4℃ for dried film before photocuring favored film formation at room temperature DSC and DMA results showed that PU-PETA is compatible with acrylic resin in the grafted resin The TG and dTG curves for Acr-g-PU-PETA samples were substantially different from the corresponding blends, and the brodened dTG peaks for photocured grafted resin suggest that there might be multiple interactions among chain segments The photocued film of the grafted resins self-emulsifying system exhibited good overall properties with polyurethane acrylate grafts ranging from 30% to 50%, and Acr-g-TPU-PETA series showed better properties than Acr-gPPU-PETA series The adhesion of the photocured film was 1～2 grade, impact strength was 50kg-cm and flexibility was 1mm The Shore hardness and the adhesion of the film decreased markedly if PETA was replaced by HEMA as photoactive group due to the lower C=C double bond content in the grafted resin The polyhydroxy amine-linked thioxanthones photoinitiators can be evenly dispersed in self-emulsifying system without color spot appearing in the dried film The films photocured with IV〓～IV〓 generally exhibited higher Shore hardness, gloss and solventresistance than that cured with Irgacure 2959 and did not induce the rusting of the iron substrate Pigmenting with titanium oxide had no effect on the photocuring The polyhydroxy amine-linked thioxanthone IV〓 and IV〓 can be new candidates for the formulation of titanium oxide pigmented photocurable water-borne coatings for their pale-yellow appearance and slight interference with the color of the coatings WB 4759 with high water-solubility coalesced and separated from the dried resin film, and even induced the rusting of iron substrate.

以丙烯酸丁酯，甲基丙烯酸-β-羟乙酯，丙烯酸及苯乙烯合成厂丙烯酸共聚树脂，并与含-NCO端基的聚氨酯丙烯酸酯接枝反应，经水性化后，得到两个系列（Acr-g-TPU-PETA和Acr-g-PPU-PETA）各5个不同配比的丙烯酸树脂接枝聚氨酯丙烯酸酯自乳化体系，粘度与水相近，为牛顿流体，固含量30％左右。光固化前干燥涂膜的玻璃化转变温度在-15℃～4℃之间，有利于室温下成膜。DSC及DMA谱表明两种树脂基本相容。Acr-g-PU-PETA的热失重行为明显不同于共混材料，接枝样dTG峰明显宽化，表明接枝样中链段之间的相互作刚有多重性，丙烯酸树脂与聚氨酯之间有一定的相容性。接枝树脂自乳化体系光固化涂层的综合性能以聚氨酯丙烯酸酯成分为50％～30％者相对较好，且以Acr-g-TPU-PETA系列更理想。光固化膜的附着力为1～2级，冲击强度为50kg·cm，柔软性多为1mm。以HEMA代替PETA作为接枝树脂的活性基团，碳—碳双键密度太低，光固化膜交联点太少，硬度和附着力均较差。多羟基胺连硫杂蒽酮光引发剂能均匀分散于树脂自乳化体系和接枝树脂干膜中，不出现色斑，光固化膜肖氏硬度一般高于以Irgacure 2959为光引发剂的光固化膜，光泽度及耐丁酮溶剂性能均有所提高，不会诱导铁基生锈，钛白着色对光固化结果几乎没有影响。本论文所合成的多羟基胺连硫杂蒽酮光引发剂IV〓与IV〓为浅黄色，对光固化涂层的颜色干扰较小，为配制钛白着色光固化水性涂料提供了新的光引发剂。水溶性太强的WB 4759在干膜中易聚结析出，且对所附着的铁基有诱导锈蚀作用。

Western blot analysis with the antiserum ATP95 revealed a significant increase of protein amounts of the V-H〓-ATPase subunits B and c of S. slasa under 100 and 400 mmol/L NaCl treatment, which gave another evidence for a salt-induced coordinate up-regulation of V-H〓-ATPase subunits at transcription and translation levels. The coordinated salt-induced increase of subunit B, H and c of V-H〓-ATPase from S. salsa at transcription and translation levels indicated an increase of V-H〓-ATPase holoenzyme amounts, which might be the reason for the increase of V-H〓-ATPase activity of S. salsa under salt stress.

400 mmol／L NaCl处理盐地碱蓬植株分离其叶片液泡膜微囊进行Western-blot分析发现盐胁迫明显诱导了盐地碱蓬液泡膜H〓-ATPase B、c亚基蛋白的表达，证明盐胁迫下，盐地碱蓬液泡膜H〓-ATPase各亚基在转录、翻译水平存在协同表达。100、400mmol／L NaCl处理亦明显增加了盐地碱蓬叶片液泡膜微囊H〓-ATPase活性，表明盐胁迫下，盐地碱蓬液泡膜H〓-ATPase各亚基在转录、翻译水平的协同表达增加了H〓-ATPase全酶的数量，进而增大了H〓-ATPase的活性。

OBJECTIVE: To explore the effects of laminin and type Ⅳ collagen on the adhesion and proliferation of Schwann cells.

目的：了解基膜粘连蛋白和Ⅳ型胶原对体外培养条件下许旺细胞的贴壁及增殖作用的影响。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。