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The HUVECs retained spindled shaped or cobblestone appearance and positively stained by anti-CD31. DAPI staining showed that 90%of scaffolds surface was reendothelialized and endothelial cells were distributed evently on the surface forming a confluent monolayer.

脱细胞支架细胞成分完全脱去,胞外基质保存完好;分离的细胞培养一周后,细胞呈梭形或多角形,核仁清晰,大小均匀,呈典型的铺路石样;免疫荧光检测可见细胞胞浆内有颗粒状红色阳性信号,说明Ⅷ因子相关抗原染色阳性;膜表面有蓝色阳性信号,说明CD31抗原染色阳性。

Fluorescence of NC16 and NC10NC at high pressure The monomer fluorescene intensity of the naphthalene chromophore in NC16and NC10NC in solid state and in LB film was decreased and red-shifted with theincrease in pressure, and a structureless excimer band at 415 nm was observed.

四。高压条件下NC16和NC10NC的荧光性质高压条件下研究了NC16和NC10NC在固体和在LB膜中的荧光性质,发现随压力增加,样品的单体荧光强度逐渐下降、红移;并在415 nm处出现萘的激基缔合物荧光。

In order to improve the stability and mechanical properties in moist state,the gelatin nanofibrous membranes were chemically crosslinked by 1-ethyl-3-dimethyl-aminopropyl carbo-diimide hydrochloride and N-hydroxyl succinimide.

采用1-乙基-(3-二甲基氨基丙基)碳二亚胺和N-羟基琥珀酰亚胺对明胶纳米纤维膜材料进行化学交联处理,改善了材料的耐水性、热稳定性和力学抗拉性能。

The effect of the introduction of sulfonic group on the improvement of conductivity and solubility in organic solvents and water as compared with polyaniline are specifically analyzed.

结合典型实例,论述了磺化聚苯胺的合成及成膜方法,着重分析了磺酸基的引入对聚苯胺电导性及其在有机溶剂和水中的溶解性的改善作用。

This paper presents a rapid method for the characterization of sulphonated condensationpolymerized products of phenolphthalein and dichlorodiphenylsulfone membrane by using infrared attenuated total reflection and diffuse reflectance spectroscopy.

采用漫反射及衰减全反射红外技术,快速鉴定酞侧基聚砜膜的改性水平,得到了磺化后—SO3H基团的红外光谱差谱。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制,酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力,酶蛋白亚基间的相互作用,酶蛋白的折叠以及内运动状态与酶活力的关系,酶蛋白发挥功能时的结构特性;运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性,热稳定性机制;确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位;确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构;确定了一个新型的染色质蛋白Cren7的溶液三维结构;正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

To explore effect of copper on MPTP in liver cell, performance, blood index, hepatic function, growth hormone and histopathological change, copper sulfate, copper methionine and tribasic copper chloride had been used in this experiment, level of copper was 11mg/kg in control group, the three levels of each copper sulfate, copper methionene and TBCC added to the basas diet were 110mg/kg, 220mg/kg and 330mg/kg copper in experimental groups.

为了全面了解肉鸡饲料中铜元素对其肝细胞线粒体膜通透性转变孔、生产性能、血常规、肝功能、生长激素以及组织病理学变化的影响,本试验选用:硫酸铜、蛋氨酸铜、三碱基氯化铜作为铜源,基础日粮对照组饲料铜含量为Feedstuff推荐的11mg/kg,三个试验高铜组分别为:110mg/kg、220mg/kg、330mg/kg.360羽1日龄AA商品代肉鸡随机分为12组,每组30羽。

SPMDs filled with triolein, and goldfish were simultaneously exposed to nine moderately hydrophobic hydrophobic chlorophenols and nine weakly hydrophobic nitroaromatics respectively, from water in laboratory continuous-flow experiments.

针对氯酚和硝基芳烃两类、各九种有毒有机污染物,在实验室内分别进行了流动体系下SPMD模拟采样动力学研究,并和鱼体富集进行平行比较,探讨了半渗透膜采样技术对中、低等疏水性污染物进行监测的可行性和机理。

The research indicated that, the structure inside the pure C8TES SAM was indeed loose and unassembled completely, the molecule density inside the SAM was very low, which would result in an ultra-low surface contact angle, only 20~21°, on the C8TES SAM surface.

我们的研究表明,由于C8TES分子头部三个乙氧基形成的空间(来源:b5e5ABe3C论文网www.abclunwen.com)位阻的影响,C8TESSAM的内部处于一种不完全组装的松散状态,所形成的自组装单分子膜内分子密度偏低,造成C8TES SAM的表面接触角偏低,只有20~21°。

When TA or Diethylene glycol terephthalate were applied to common metabolism, F4 and p.ch were used to degrade uncrystalline Polyethylene terephthalate film, which was prepared by resolving PET fiber with organic solvent, at temperature of 30℃, shaking frequency 140 r/min in HZQ-C air bath oscillator from one month to three months, and then the scanning electron microscope was applied to observe faces of PET film.

在菌株最佳生长条件下,分别以TA和DTP作为共同代谢基质,研究F4和p.ch菌株降解PET膜。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。