基膜

The effect of the toxin on protoplasmic membrane protein was studied by the method of fluorescence, using 8-anilino-1 -naphthalene-sulfonic acid as probe and taking the mesophyll protoplasts from maize leaves as the research system.

以玉米叶片原生质体为研究对象，以ANS(1-苯胺基-8-萘磺酸)作荧光探针，用荧光法研究了Ⅱ-3与质膜蛋白的互作。

RCF is an ideal method that can quantifiably evaluate the bonding strength. However, small balls are necessary for thin films.

滚动接触疲劳法能够得到膜基界面强度的定量值，是比较理想的结合强度测试方法。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Spikelets with 1 floret, mostly laterally compressed, rachilla extension absent, falling entire; glumes absent or reduced to small scales at base of floret; lemma membranous to leathery, keeled, 5–10-veined, entire, with or without a straight awn from the apex; palea resembling lemma but narrower, 3–7-veined with central keel.

小穗具1小花，多数侧面压扁，小穗轴扩展无，变为全缘；颖片小花的无的或退化至小鳞片在基部；外稃膜的到革质，翻转，5-10脉，整个，有或没有来自顶的一直的芒；内稃象外稃但是狭窄，3-7脉与中心的龙骨一起。

Fruit dehiscent siliques or silicles, linear, oblong, obovoid, or ellipsoid, terete or slightly 4-angled, sessile or shortly stipitate; valves with an obscure to prominent midvein, glabrous, smooth or subtorulose; replum rounded; septum complete or basally perforated, membranous; style obsolete or short and less than 1 mm; stigma capitate, entire.

果开裂长角果或短角果，线形，长圆形，倒卵球形，或椭圆形，四棱形的圆柱状的或稍，无梗或具短柄；裂爿具一不明显对突出中脉，无毛，平滑或者；假隔膜绕行；基部的隔膜完全的或具孔，膜；花柱废退的或短和不到1毫米；柱头头状，全缘。

Lowermost leaf sheath retrorsely strigose at base, uppermost sheaths closed 1/6–1/3 of length; blade grayish green, flat, folded, or involute, papery, 2–8 mm wide, abaxially smooth, adaxially nearly smooth to densely scabrid along prominent veins, apex slender prow-tipped; ligule white or off white to brownish or yellowish, membranous-papery.

最下的叶鞘反曲的具糙伏毛的在基部，最上部的鞘关闭长度中的1/6-1/3个；叶片灰绿色，平，折叠，或内卷，纸质，毫米宽，背面平滑，正面近平滑兑一沿着突出脉浓密粗糙，叶舌白色或者离或者淡黄，膜似纸白色的到带褐色。

The major outer membrane protein B, the most abundant surface-exposed protein, is one of the principal rickettsial candidate antigens for developing diagnostic agents and subunit vaccine.

外膜蛋白B是立氏立克次体最主要的表面蛋白抗原，被认为是发展落基山斑点热诊断试剂和亚单位疫苗的靶分子。

Sepals oblong, ascending or spreading, base of lateral pair not saccate, margin membranous.

萼片长圆形，上升的或开展，侧的对的基部不囊状，边缘膜质。

Sepals oblong, base of lateral pair not saccate, margin membranous.

萼片长圆形，侧的对的基部不囊状，边缘膜质。

Sepals ovate, base of lateral pair not saccate, margin not membranous.

萼片卵形，不囊状的侧对的基部，边缘而不是膜质。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。