基因的

Because the dimeric form is the active form to exert its biological activities, many trials failed to express TGF β s in prokaryotic systems because of their complex folding manner and disulfide patterns. In 1991 Cerletti and coworkers from Ciba-Geigy Corporation in Switzerland successfully finished the functional expression of human TGF β 2 in bacteria, but the details are protected by the patent.

由于双体是其发挥生物活性的形式，TGF β复杂的折叠方式和二硫键配对使其早期原核基因工程研究都以失败而告终。1991年，瑞士Ciba-Geigy公司的Cerletti等成功完成了人TGFβ2基因的细菌表达，但其技术细节被专利加以保护。

Although direct lethal effects on cotton boll worm couldn't be generated in tobacco plants transformed with cholesterol oxidase full gene vector or mature enzyme gene vector, inhibitory effects on the growth and development of cotton boll worm have been observed to a certain degree.

转单价胆固醇氧化酶全酶基因和成熟酶基因的转基因烟草植株均不能对棉铃虫产生直接的致死作用，但能使棉铃虫的生长发育受到一定程度的抑制。

Genes count for little by comparison: being a genetic Leonardo was not enough to compensate for having been born near Milan instead of Florence.

比较起来，基因的作用无足轻重，具有达芬奇的遗传基因，并不能弥补住的地方离米兰近、离佛罗伦萨远带来的影响。

Third, Construction of the physical map covering the target gene using large fragment libraries.

只要目的基因的表型可以鉴定，并能通过遗传连锁分析将它的位置定位在染色体上距离很近的两个分子标记之间，它可用于任何基因的克隆。

Abstract］ AIM: To construct recombinant vectors expressing siRNA that target CD59 gene and a stableinhibit cell line A2780 in order to analyze the role of CD59 in the protection to complementmediated cytolysis.

摘 要］目的：构建针对人CD59基因的siRNA表达载体并筛选稳定细胞系，观察CD59基因改变后对补体溶破的抵抗作用的变化，探讨CD59在肿瘤细胞免疫逃逸及相关信号转导通路中的作用。

At present, most genes were isolated by screening the cDNA or DNA library with theprobes homologous to sequences of dicot plants, and their functions were identified by themorphology of floral organs in transgenic plant.

目前研究手段主要是利用双子叶植物的同源序列来筛选水稻的cDNA文库，获取相关基因，然后根据转基因植物花器官的表型来验证该基因的功能。

In yeast, protein complexity may serve as a better indicator of gene dispensability than do duplicate genes.

对酵母菌的基因可移除性来讲，蛋白质的复杂性应该比重复基因的影响来的重大许多。

METHODS: Subtractive cDNA DNA library of PDLF was constructed with a recently developed gene cloning technique that is based on PCR and subtractive hybridization and then screened by restriction analysis and reverse southern dolt blot.

采用基于PCR和消减杂交的基因克隆技术构建体外培养的人PDLF与GF差异表达基因的扣除文库，采用酶切和反向杂交的方法筛选目的克隆，并进行测序和计算机分析。

In this review, we provide a general background of the Pax genes, highlight the role of Pax proteins playing within specific tissues in terms of embryological development, and give a brief introduction of alternatively spliced isoforms of Pax protein identified.

文章简述了Pax基因的相关背景知识，详细介绍Pax1—Pax9调控在胚胎组织发育中的各项功能，并列举了现已确定的Pax基因在不同物种中的选择性剪接产物。

Although overexpression of Hspa5 does not cause detectable defects, its knockdown by injecting Hspa5 morpholino antisense oligonucleotides results in slower epiboly during gastrulation and dorsalized phenotypes at 24 hpf, including smaller and curled tail and absence of caudal ventral fin. When Hspa5 expresison in embryos is knockdown, the expression of the dorsal markers gsc and ntl is enhanced while the expression of ventral markers is reduced.

尽管在胚胎中过量表达Hspa5对胚胎发育没有明显影响，但是利用反义吗啉环寡核苷酸抑制胚胎中Hspa5的表达会导致胚胎外包变得缓慢，24hpf出现尾部变细或卷曲、缺少腹鳍等背部化的表型，同时gsc和ntl等背部标记基因的表达增强，但eve1等腹部标记基因的表达减弱。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。