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Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed.

以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌,采用Tn5转座子诱变技术,以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌,使Tn5插入并整合到冰核细菌基因组中,使冰核基因的线性连续性被破坏,失去冰核活性。

According to recent researches, intracellular signalling pathway systems involved in appressorium differentiation is related with cAMP pathway and MAP pathway. In this research, 21 bacteria strains and Chaetomium cupreum strains were assayed for their antagonism to appressorium formation in M.grisea.

随着稻瘟病菌一系列致病基因的克隆及功能明确,附着胞形成的信号传导途径包括:cAMP途径和MAP途径,这些途径中基因的失活都影响附着胞的形成。

Its alterations of phenotype and structure have been further studied at whole, organic and cellular levels. By means of backcross between wildtype and the mutant, its genetic background has been analyzed.

本文在整体水平、组织水平和细胞水平上深入研究了该突变体的表型和结构的变化、通过突变体与野生型之间的杂交对其遗传背景进行了分析,并运用分子生物学手段对突变体相关基因进行了克隆和定位,初步探讨了该基因的表达模式和功能。

We determined the mitochondrial DNA sequence of S. yangtsekiense and discussed the phylogenetic status of freshwater crabs in Brachyuran according to gene arrangement features and sequence data sets. We also sequenced regions of mitochondrial COI and ND5 genes in order to address the genetic structure of S.

本文以长江华溪蟹为代表,通过对线粒体基因组序列的测定,从基因序列和排列顺序2个方面探讨淡水蟹类在短尾类中的系统地位;并利用COI和ND5 基因的部分序列数据,对我国长江华溪蟹种群的遗传结构进行了初步研究。

Exploring novel genes and gene combinations are of primary importance in plant drought-tolerance and water-saving research. Based on the EST sequence of MYB gene obtained in the research on molecular basis of drought-tolerance and water-saving in broomcorn millet Panicum miliaceum L.

根据在糜子抗旱节水分子基础研究中获得的一个糜子 MYB 基因的EST序列,以其序列及水稻MYB18基因的序列为基础设计引物,扩增得到1 739 bp的全长基因组序列。

Therefore, the technique of gene SOEing is a simple and effective method for gene recombination and mutation in vitro, which is significant to study structurally and functional characteristic of gene in high plants.

因此,重叠PCR技术是一种简单有效的进行体外基因重组和突变的方法,对于研究高等植物功能基因的结构功能特性具有重要的意义。

An organism that has different alleles at a particular gene locus on homologous chromosomes.

杂合体在同源染色体的某一特定基因位点上有不同的等位基因的有机体。

We are also interested in developing rice plants with broad-spectrum disease resistance and stress tolerance for agricultural application.

克隆控制植物器官生长、分化的重要基因,研究这些基因的调节功能和它所涉及的调控网络,最终将拟南芥研究成果向经济作物转化。

The successful cloning and expressing of vp 1 gene become thebasis for the immunological studies of VP1 protein and themanufacture of genetic engineering vaccine of CAV.

vp1基因的成功克隆与表达,为VP1蛋白的免疫学研究和CAV基因工程疫苗的研制奠定了基础。

This study will provide new insight on the function of HBV preS/S gene.

3'末端缺失的preS/S基因能够在胞内表达,此研究为基因的功能研究奠定了一定的基础。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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