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In vivo-induced genes spd_0300, spd_0414, spd_0622, spd_1663, spd_1719, spd_0235, spd_0873, spd_1672 regulated by transformation were screened out and they may participate in the processes such as growth regulation, temperature reception, carbohydrate metabolism, and lipoids metabolism.

筛选出受转化调控的体内诱导基因spd_0300、spd_0414、spd_0622、spd_1663、spd_1719、spd_0235、spd_0873、spd_1672,它们可能参与生长调节、温度感应、糖脂代谢等环节,表明细菌转化可通过调节某些体内诱导基因的表达来增强细菌的毒力。

The prawn lysozymes are presumed to be the non-calcium binding family of chicken-type lysozyme because they have two conserved catalytic sites Glu~(51) and Asp~(68),as well as eight structural Cys residues,which are highly conserved among the species of chicken-type lysozymes but lack of three Asp residues at the site 101,106 and 107,which are conserved sites in calcium-binding chicken-type lysozyme.

两种沼虾溶菌酶都具有c-型溶菌酶典型的两个酶活性位点(Glu51)和(Asp68),以及8个保守结构氨基酸残基Cys,且在101、106和107位上缺少Asp,因而推测本实验所克隆的两种沼虾溶菌酶基因属c-型溶菌酶基因的非钙结合亚型。

EF116490. Characterization and comparison of this sequence with mouflons, goat, cattle and European bison orthologues were also conducted. Results showed that: 1 The 5'-flanking region contained many potential transcriptional factor binding sites such as those for C/EBPb, C/EBP, SP1, Cap, USF, HFH-2, HNF-3b, and Oct-1, which might have an important effect on transcription activation and regulation as well as tissue-specific expression.

EF116490,在分析其序列结构特征的基础上与GenBank中摩弗伦羊、山羊、普通牛、欧洲野牛进行了比较基因组学和系统进化研究,结果表明:(1)欧拉型藏绵羊GHR基因启动子P1区存在C/EBP、C/EBPb、SP1、Cap、USF、HFH-2、HNF-3b、Oct-1等多个潜在的转录因子结合位点,可能与GHR基因的转录调控和起始以及特异表达有关。

Bp cDNA encoding L-galactono-1,4-lactone dehydrogenase fragment was cloned from fruit of Rosa. roxburghii Tratt by the method of RT-PCR on the basis of the homologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc.

根据GenBank中登录的拟南芥、花椰菜、甘薯和草莓等植物的半乳糖内酯脱氢酶基因保守序列设计引物,采用RT-PCR法,从刺梨果实中扩增出该基因的cDNA片段855bp并测序。

The vector will be the foundation of the next research on the transfect of cells .

为了进一步研究noggin基因的神经诱导功能和在细胞分化过程中的表达情况,本研究利用基因工程的方法构建pCS2+[Tα1]-Noggin载体。

To study ovarian gene expression in teleosts, we constructed an adult ovary cDNA library of tilapia, Oreochromis mossambicus, and analyzed the gene expression profile using an expressed sequence tag-based strategy.

为了研究硬骨鱼卵巢的基因表达,本文构建了成年莫三比克吴郭鱼的卵巢cDNA文库,利用表达序列标签策略分析了卵巢基因的表达。

All three exons including splice sites were screened. Results Two of the 25 patients with advanced retinopathy of prematurity showed point mutation in the non-read frame of the third exon of the ND gene. Similar analyses of the first and second non region failed to detect additional mutation, including splice bites. None of the controls resealed similar mutation.

结果 2例患儿的第3外显子的非读码框架基因序列经银染后可见2条异常条带出现,并经直接测序验正发生基因的点突变由T到C,而外显子1及2以及其剪接区均未发现突变对照组未发现相同突变。

Methods A CMT2L proband with 423 mutation in HSP22 gene had been studied and reported by the present authors.

方法应用聚合酶链反应和DNA直接测序方法,对1个发现HSP22基因423突变的CMT2L家系外的114个CMT家系先证者进行了HSP22基因的突变分析。

Expression of colicin and immunity genes in translational level has not been investigated extensively in the ColE operon.

C0lE7操纵子中,有关大肠杆菌素基因与免疫蛋白基因的转译表现,至今还详尽的研究,本论文利用西方对这个问题做进一步的探讨。

Coli lacZ gene at icp34.5 gene (RL1), an antiapoptosis and neurovirulence gene, which was constructed in our lab, is one of the oncolytic viruses.

本室构建的缺失凋亡抑制基因icp34.5并插入LacZ基因的单纯疱疹病毒就是一种有靶向性的溶瘤病毒。

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