基因的

The aberrant phenotypes were observed in the anther of tobaccotransformed with ZM401 sense construct: the inflorescence growth and floral axiselongation are suppressed; the flower buds falling or flowers abscission earlier wereobserved. The degradation of tapetum are not observed at the binucleate pollen stagewhich corresponds to the mature stage, although the tapetal cells of untransformedtobacco anther at the same stage have already degenerated completely anddisappeared.

对转基因烟草进行形态学、组织学分析结果表明，ZM401基因以反向方式整合的转基因烟草表现一切正常，而含有ZM401正义基因的转基因烟草在表型和组织学上都出现了异常：花序生长缓慢，花序轴变短，生长过程中花逐渐脱落，花序轴褐化枯死；花药绒毡层退化延迟，药室内壁在雄配子体发育后期不能进行带状加厚，阻碍了花粉粒的释放。

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞，然后用携带lac-Z基因的腺病毒载体转染肌干细胞，待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围，分别于注射后5、15d，处死大鼠取出膀胱颈及后尿道，行组织学检查及X-gal染色。

Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞，G418抗性筛选3-4周后，经PCR扩增和报告基因EGFP表达检测，得到稳定整合外源基因的转基因供体细胞系，为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

A pair of specific primers was designed according to the reported cDNA sequence of the DC-ACO(Dianthus caryophyllus L.ACC Oxidase),and a partial sequence of the gene was amplified from genomic DNA of a carnation variety 'Master' by PCR.

据已报道的香石竹氨基环丙烷羧酸氧化酶基因的cDNA序列设计特异引物，以香石竹品种&Master&基因组DNA为模板，用PCR方法克隆出香石竹ACO基因的部分序列。

We composed the rhoa-gene of Wistar rat with the plasmid of pAdTrack-CMV to get the recombinant plasmid. On the basement, we can use the catholyte lipofetamine to transfected the neural stem cells , In the following, we observe the expression of the rho-gene and the geng how to act the motor neuron and NSCs.

本试验用克隆出的大鼠rhoa基因构建到质粒pAdTrack-CMV中，获得重组质粒pAd-rhoa，用阳离子脂质法介导转染到神经干细胞，观察目的基因的表达及其对运动神经元和神经干细胞的作用。

Crocin can induce the significant alteration of gene expression profile of T24 cell. It is suggested that the widly konwn anti-tumor effects of crocin are medicated at least in part by regulating the cell cycle controlling gene expression.

藏花素可以诱导T24细胞基因表达谱广泛的改变，并可能通过调控细胞周期相关基因的表达来抑制T24细胞的增殖。

Objective To construct retrovirus vectors carrying IL17 or siRNAIL17 genes with Thy1.1 gene and determine the infected ability of the retrovirus vectors to diabetogenic BDC2.5 T cells.

目的 构建含有Thy1.1细胞表面抗原(Thy1.1 cell surface antigen，Thy1.1)基因的携带IL17或siRNAIL17基因的逆转录病毒载体，并观察逆转录病毒对致糖尿病性BDC2.5 T细胞的转染能力。

Taxol have been used in clinic therapy of breast cancer. Generally speaking, taxol can accelerate the-assemble of microtubule, stabilize microtubule from its discomposing, make mitosis stagnate at G2/M, sequentially interdict the normal mitosis and proliferation of cells, induce cell apoptosis; Besides, a direct function of taxol on cell signal and gene expression, activations of apoptosis genes and expression of proteins can induce apoptosis.

紫杉醇已应用于乳腺癌的临床治疗，一般认为紫杉醇能增强微管聚合，稳定微管防止解聚，使有丝分裂停滞在G2/M期，从而阻断正常的有丝分裂和细胞增殖；除此之外，紫杉醇可以直接在细胞信号和基因表达上发挥作用，活化多种凋亡相关基因的表达，从而引发细胞凋亡。

Analysis of PcG mutants shows that the PcG system represses genes required for anteroposterior, dorsoventral, and proximodistal patterning of imaginal discs and that it also represses cell cycle regulator genes.

对PcG突变体研究发现，PcG系统表达基因在幼虫发育的前端、背腹以及远端模式都是必须的，同时可抑制细胞分裂调节基因的活性。

I found that the major distinction for gene duplicability in protein complexity is between hetero-complexes, each of which includes at least two different types of subunits, and homo-complexes, which include monomers and complexes that consist of only subunits of one polypeptide type.

接下来，我利用功能性基因体及蛋白质结构资料，探讨蛋白质复杂性（蛋白质次单元种类的数目，protein complexity）对基因的可移除性及可复制性的影响。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。