基因的

We isolated a transcriptional enhancer element from the genome of an extinct marsupial, the Tasmanian tiger ( Thylacinus cynocephalus or thylacine), obtained from 100 year-old ethanol-fixed tissues from museum collections.

艾契尔曾试图复制袋狼。在实验过程中，研究人员将袋狼的一个基因植入老鼠胚胎后，检查该基因的功能，结果发现，发育中的老鼠软骨原骨已经具有功能，最后并可以形成骨骼。

The genes gB,gC and gD amplified from infectious laryngotracheitis virus Chinese Wanggang strain were cloned into the Eco RⅠ site of the eukaryotic expression vector pCAGGS,re-spectively. The positive recombinant plasmids were screened by transforming into E.coli DH5 α cells,which were named pCAGG-gB,pCAGG-gC and pCAGG-gD respectively after identification by restriction endonuclease analysis and sequencing analysis.

将传染性喉气管炎病毒王岗株的gB、gC、gD基因分别克隆到真核表达载体pCAGGS的 Eco RⅠ位点，经过酶切、测序分析，筛选鉴定出含有gB、gC、gD基因的重组质粒，分别命名为pCAGG-gB、pCAGG-gC、pCAGG-gD。

The effect and variation of major gene are estimated by the maximum likelihood method implemented via expectation-maximization algorithm. Major gene is tested with the likelihood ratio test statistic.

该方法以EM算法实现的极大似然估计方法进行主基因的效应估计，以似然比统计量进行主基因的各种遗传假设检验。

In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase gene in Epstein-Barr virus transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT－PCR protocol and analysed by polyacrylamide gel electrophoresis.

为检测用EB病毒转化建立的永生性人B淋巴母细胞株中N5，N10－亚甲基四氢叶酸还原酶基因的表达及其cDNA序列，用EB病毒转化人外周血B淋巴细胞，建立永生性细胞株后，从培养细胞中提取总RNA，用RT-PCR法扩增MTHFR基因cDNA的不同片段，进行PAGE电泳分析和cDNA序列测定。

And the rats in the control were executed at 5 months to observe the develope of pancreatic cancer by macrograph and microscopy, The EnVision TM immunohistochemistry was used to assay the expression of EZH 2 and PTEN in above pancreatic specimens.

较大剂量DMBA置入胰实质内可获得较高胰腺癌发生率，TSA能抑制胰腺癌的发生和生长；EZH 2基因的激活及PTEN基因的失活在大鼠胰腺癌发生中可能起关键作用。

These genes exact expression is activated by flower meristem identity genes and controlled by various positive and negative regulators.

这些基因的精确表达需要花分生组织特异性基因的激活和多个正负调节因子的调控。

Examination of the 100 genes most specific to each brain region showed that expression in some regions, such as the hypothalamus and midbrain, oerlaps significantly with expression in others.

人们对100种脑区域特异基因的表达进行检测发现，下丘脑和中脑中的某些基因同时在脑的其他区域表达。

The modificative effects of HDACi seem to be dependent upon the type of tumour cell rather than the specific HDAC inhibitors used.

HDACi对基因表达的调控不是通过直接修饰DNA序列，而是通过协助DNA紧密地结合在组蛋白氨基端上阻止肿瘤抑制基因的转录和表达来发挥作用的。

Thus, each cell population displays a vast heterogeneity in patterns of mono- and biallelic gene expression, providing numerous combinatorial patterns of gene expression.

因此，每个细胞群在单一和等位基因的表达模式上显示出巨大的差异性，提供了基因表达的许多组合模式。

In this study,we constructed two plant expression vector with rip gene from barley and linked to two promoters which have high promoting efficiency in monocotyledonous plants,respectively,and then transferred to rice mediated by Agrobacterium Tumefaciens.

本研究构建了分别由单子叶植物中启动效率较高的PactⅠ和P2×35s启动子调控的大麦核糖体失活蛋白基因的植物表达载体，通过农杆菌介导法转化水稻，经PCR检测初步验证了rip基因已导入水稻。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。