基因的

Methods: Unrepeated MRSA strains from clinical specimens were divided into two groups: C-MRSA and H-MRSA, We used Chelex 100 to get DNA template and amplified mecA gene by PCR. The DNA templates of MecA were used to RAPD(random amplified polymorphic DNA), then we got the RAPD-DNA fingerprint-maps, randomly sampled 20 strains from each group as study objects.

收集临床MRSA标本，将其分为社区获得性MRSA与医院获得性MRSA两组，用Chelex 100裂解法制备DNA模本，PCR扩增mecA基因，选择mecA基因的DNA模本，用RAPD技术扩增出各菌株的RAPD-DNA指纹图谱。

In recent years, with the development of the molecular cloning technology, genetic engineering and bioinformatics, the study of vitellin gene will provide a new method to biological control of pests.

近几年，随着分子克隆技术、基因工程手段和生物信息学的发展，对卵黄蛋白基因的研究将为寻找害虫生物防治提供新途径。

We chose the gene, vomeronasal type 1 receptor(V1rc), as the first candidate gene and western blotting was employed to quantify the V1rc protein level in 0hr, 3.5hr, 7hr, 12hr after training.

我们挑选 vomeronasal type1 receptor (V1rc)做为第一个候选基因，以西方点墨法定量V1rc基因的蛋白质表达，分别记录在训练后立即、3.5小时、7小时以及12小时，四个牺牲时间点的V1rc表达量。

The point mutations of the 12th coden of ras gene and p53 E5 and E7 gene were studied in 40 cases w ith laryngeal squamous cell carcinomas by PCR-SSCP(polymerase chain reaction-s ingle stranded conformational polymorphism).

应用多聚酶链反应-单链构像多态性分析方法分析40例喉鳞状细胞癌H-ras，K-ras，和N-ras基因的第12密码子，p53 基因第5，7外显子中点突变的情况。

Simultaneously, we also found their geminating speeded up, leave chlorophyll content improved by 13%, flowering date moved up and the boll number increased significantly. This work had proved that the transgenic cotton expressing Vitreoscilla hemoglobin showed both yield improvement and water logging resistance.

本研究首次报道了转透明颤菌血红蛋白基因的棉花在增产和抗涝性提高两方面具有作用，并注意到了叶绿素含量测定可作为检测vgbM基因表达的指标，为进一步获得增产转基因农作物提供了研究基础。

Objective: The purpose of this study was to determine whether potentiating induction of apoptosis in cervical cancer cell line SiHa lacking full-length FHIT transcripts and endogenous FHIT protein by cisplatin via introduction of extopic FHIT gene. Meanwhile, to investigate if the apoptosis of the SiHa cells is induced by FHIT gene.

目的 验证外源性脆性组氨酸三联体抑癌基因的导入是否诱导宫颈癌SiHa细胞凋亡，观察FHIT导入与顺铂联合应用诱导宫颈癌SiHa细胞凋亡的作用是否有协同效应，为联合基因治疗和化疗药物治疗人宫颈癌提供实验依据。

A system which predetermines what each gene must do or how many genes there are can only evolve to predetermined boundaries.

一个预先确定每个基因必须做什么，或有多少基因的系统只能演化至预先确定的界限。

Hass-PBP、Hass-GOBP-1、Hass-GOBP_2 genes were only expressed in the antennae of adult,but not in the other parts of body,which including proboscises,head without antennae,thorax,abdomen,leg,wing;besides,Hass-PBP gene was only expressed in the antennae of male adult,while Hass-GOBP_1、Hass-GOBP_2 genes were expressed in the antennae both of male and female adult.

烟夜蛾3种气味结合蛋白基因的时空表达：(1)烟夜蛾Hass-PBP、Hass-GOBP_1、(来源：A3cBCfc6c论文网www.abclunwen.com)Hass-GOBP_2基因在卵期、幼虫期、蛹期均不表达，只在成虫期表达，且表达量与成虫的日龄无关。

Kelch-like ECH-associated protein-1 (Keap1) normally sequesters Nrf2 in the cytoplasm in association with the actin cytoskeleton, but upon oxidation of cysteine residues Nrf2 dissociates from Keap1, translocates to the nucleus and binds to ARE sequences leading to transcriptional activation of antioxidant and phase II detoxifying genes.

在正常情况下，Kelch样环氧氯丙烷相关蛋白-1 (Kelch-like ECH-associated protein-1， Keap1)可将Nrf2与肌动蛋白细胞骨架一起收集在细胞质内，但是在半胱氨酸残基发生氧化的情况下，Nrf2和Keap1脱离，进入细胞核并与ARE结合，从而激活多种抗氧化基因和II相解毒基因的转录。

Kelch-like ECH-associated protein-1 (Keap1) normally sequesters Nrf2 in the cytoplasm in association with the actin cytoskeleton, but upon oxidation of cysteine residues Nrf2 dissociates from Keap1, translocates to the nucleus and binds to ARE sequences leading to transcriptional activation of antioxidant and phase Ⅱ detoxifying genes.

在正常情况下，Kelch样环氧氯丙烷相关蛋白－1(Kelch-like ECH-associated protein-1， Keap1)与Nrf2耦联，并与肌动蛋白细胞骨架结合被锚定于胞浆，但是在半胱氨酸残基发生氧化的情况下，Nrf2和Keap1解耦联，进入细胞核并与ARE结合，从而激活多种抗氧化基因和Ⅱ相解毒酶基因的转录。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。