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Methosd The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product .

先从并殖吸虫病患者痰中分离出虫卵,然后PCR扩增出虫卵中完整的核糖体DNA第二间隔区基因(ITS2 ),并直接用于测序从而获得该基因的核苷酸序列。

RT-PCR analysis showed that a 239bp segment could be detected in CD gene transduced pancreatic carcinoma cells, but not in parental Patu 8988、SW1990 cells.

加入前药5-FC后,转CD基因的胰腺癌细胞可被杀死,而周围未转CD基因的胰腺癌细胞也可通过&旁观者效应&被杀死。

Recombining the plasmid PcDNA3.1-sTNFRⅠ.sTNFRⅠin cell culture supernatant was detected by ELISA and sTNFRⅠblockage of TNFαin pericementum cell by MTT assay.

RT-PCR方法筛选目的基因,并构建质粒;ELISA方法检测牙周膜细胞转染PcDNA3.1-sTNFRⅠ后培养上清中目的基因的表达;MTT检测目的产物是否在牙周膜细胞中有抑制TNF-α的作用。

Finally we studied the secretion expression of TGF β1 mature peptide gene in the periplasm and extracellular medium respectively.

在胞内表达TGFβ1基因的同时,还研究了TGFβ1基因在周质和胞外培养基中的分泌表达。

Pheny lalanyl tRNA synthetase,eukaryotictranslation elongation 〓 genes may be related directly to changes ofphenotypes of induicible cells.

这些基因的变化不仅为基因治疗靶点的选择提供了依据,而且为寻找导致肿瘤发生主要因素提供参照。

The research results indicated that in the genetic background of weak photo-sensitivity of flowering, photoperiodic sensitive genic male sterility and fertility restoration were controlled by two sep...

结果表明,在弱感光型遗传背景下光敏不育性及其育性恢复受两对独立的细胞核隐性主基因控制和修饰基因的作用。

There is attaching much importance to impact of population genetic structure in China upon polygenic disease research.

在对我国群体遗传结构研究的基础上开展我国多基因病相关基因的研究课题日益得到重视。

Methods: We used a 5075 gene cDNA microarray to examine gene expression in peripheral blood mononuclear cells from two type 2 diabetes patients with distal symmetric polyneuropathy, two type 2 diabetes patients without distal symmetric polyneuro...

采用包含5075个功能已知人类基因的cDNA芯片检测2名2型糖尿病并发远端对称性多神经病变患者,2名2型糖尿病不伴远端对称性多神经病变患者以及2名年龄和性别匹配的正常个体外周血单个核细胞基因表达谱。

A single point mutation in intervening sequence 2 (IVS2) of human mitochondrial arginyl-tRNA synthetase gene causes abnormal cleavage of its transcript, resulting in pontocerebellar hypoplasia.

人线粒体精氨酰-tRNA合成酶基因2号内含子中的一个单点突变导致该基因的转录本被异常剪接,造成脑桥小脑发育不全。

CEA promotor could control the expression of EC-CD gene in CEA positive colorectal carcinoma cells specifically in vitro.

CEA启动子能够控制EC-CD基因专一性地在CEA阳性的结肠癌细胞中表达,从而实现EC-CD基因的靶向性作用。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法,同时,采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。

Hey Big Raven, The individual lies dont matter anymore - its ALL a tissue of lies in support of...

嘿大乌鸦,个别谎言的事不要再-其所有的组织的谎言,在支持。