基因的

The major gene of CTXΦ receptor Tcp (toxin-coregulated pilus), tcpA, was detected by polymerase chain reaction analysis.

然而在本实验室研究中，于不含ctxAB基因的El Tor型霍乱弧菌中发现部分菌株含有RS基因同源序列，为此对这些菌株作CTXΦ基因组的进一步研究。

However, plastids only have a semi-autonomous transcription and translation machinery. The transcription of endogenous plastid genes is largely dependent on nuclear-encoded transcription factors.

然而， 质体仅具有半自主性转录和翻译机制，其内源基因的转录要依赖于核基因编码的转录因子。

In order to study the expression and functional characteristics of dehydrin gene during different growth, and make the polycolonal antibody, using wheat plumelet under the water deficit condition as experiment material, and its total RNA was extracted. The target dehydrin gene through RT-PCR was got, connect to the cloning vector PUCM-T, recombination expression plasmid PET-32a-wzy1-1 was constructed according to recombination of cloned vector PUCM-T-WZY1-1 of wheat dehydrin gene in this experiment.

为研究小麦在不同时期脱水素基因的表达情况和生物学功能及抗体制备，以小麦幼芽为材料，经干旱胁迫处理后，提取总RNA，通过RT-PCR得到小麦类脱水素基因片段(WZY1-1)，再连接至克隆载体PUCM-T，并成功构建重组表达质粒PET-32a-wzy1-1，将阳性重组质粒转化于受体菌BL21(DE3)感受态细胞中，经IPTG诱导表达，进行表达产物的聚丙烯酰胺凝胶电泳检测。

Basically, what we want to do interms of genetic studies, is to identify genes which predispose.

基本上，我们想做的是基因的研究，是要识别出哪个是易感基因。

Now targeted disruption of the Jhdm2a gene in mice shows that the enzyme is involved in spermatogenesis and regulation of transition nuclear protein and protamine genes.

现在对小鼠Jhdm2a基因的定向破坏表明，这种酶涉及精子生成和转换核蛋白及鱼精蛋白基因的调控。

Thus we conclude that the expression patterns of the putative PIN gene may relate to its function.

由此我们推测，该基因的这种表达模式与基因的功能相关。

However, there are still several safety concerns about the use of a DNA vaccine, which include the possibility of integration into the host genome, adverse immunopathology, and anti-DNA autoantibody induction.Attempting to solve some of these problems, eukaryotic expression plasmids pTL-8/ AMA-1 and pTL-8/ AMA-1 which express trans-activator or reverse trans-activator, respectively, and AMA-1 gene of Plasmodium falciparum were constructed by using tetracycline regulable system.

首先构建了恶性疟原虫顶第四军医大学硕士学位论文端膜抗原1(AMA一1)基因和反式活化因子基因的真核表达质粒pTL一8/灿认一l和PTL一8/灿认一1，并大量制备这两种质粒及表达转录抑制子的质粒pUHS6一1。

In order to elucidate the action mechanism of Rf gene in this system, many researchers have been contributing themselves to map and clone fertility restorer genes in recent years.

为阐明恢复基因在这一系统中的作用机理，众多研究者开展了恢复基因的定位和克隆研究。

In temperature and illumination combinations,the expression of per and tim gene did not present remarkable rhythmicity.

温度(25℃、20℃、15℃)和光照完全交叉设计组合之间，per基因和tim基因的表达水平也没有出现明显的变化。

Methods Six recombinant plasmids with shRNAs targeting the PDE5A3 gene of Homo sapien were constructed.

方法构建6个靶向人PDEsA3基因的短发夹RNA重组质粒，转染人阴茎海绵体平滑肌细胞48 h后，逆转录-聚合酶链反应及Western blot检测PDE5A3基因的表达抑制效果。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。