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The recombinant baculovrius expressing the human antibody genes was isolated by co-transfection recombinant plasmid DNA with liner baculovirus DNA and purified by plaque assay, the intact recombinant human antibodies IgG-IV-2 and IgG-IV-6 was expressed in insert cells sf-9 after recombinant baculovrius infection and purified by protein G affinity chromatography.

通过空斑纯化,获得表达人抗体基因的重组杆状病毒,感染昆虫细胞进行抗体的全基因表达,表达产物经G-蛋白亲和层析纯化。

Gene gun transduction of Kras antisense gene into carcinoma cell can significantly inhibit cell growth of Kras mutant pancreatic carcinoma cell lines.

转导Kras突变特异性反义基因后,具有突变型Kras基因的胰腺癌细胞系的生长都受到明显抑制。

It means that decreased expression of Adipo R2, Krs, TNF-αmight be relateted to the action of lipid metabolism by EA treatment, and the down-regulation of Utr gene expression was might be involved in cell damage after simvastatin treatment. To confirm the effect of simvastatin on the changes of gene expression and the state of cell growth, C2C12 was treated in 0, 1, 3, 10, 30 and 100μmol/L of simvastatin.

在正常组、高脂组、辛伐他汀组、针治组、针预组、针非组6个组中,AdipoR2和Krs在针治组表达量最低,TNF-α在针非组中最低,Utr在他汀组中最低,与差异基因的筛选结果一致;同时也说明AdipoR2、Krs、TNF-α在针刺组中下调表达可能与其降脂作用有关;而Utr基因在他汀组中表达减少,可能与该药对肝细胞的损伤作用有关。

Although the frequency of the B allele was much lower than that of the A allele, likelihood ratio test showed that the gene frequencies were in Hardy-Weinberg equilibrium.

群体中B等位基因的频率很低,但似然比检验结果显示群体的基因频率处于哈代-温伯格连锁平衡状态。

In order to further into research the effect of trans-acting factor TGA1a on expression of exogenous gene in the transgenic plant progeny, different transgenic plants were grown and analyzed. The result of experiments revealed that the separation of resistant gene in the transgenic plant progeny was consistent with Mendel's law of segregation.

对子代植株中gusA基因表达水平进行分子检测和GUS活性测定结果显示,反式调控系统控制下的gusA基因的mRNA转录水平和GUS活性均明显高于对照。

The result showed that the inheritance of Bar gene was a single dominant gene and accorded with the law of Mendelian segregationbasically.

结果表明,抗除草剂转基因小麦杂交与回交后代Bar基因的遗传,符合1对显性基因的分离比例和孟得尔遗传规律。

At the same time, however, the propensity to choose someone with slightly similar genes can also avoid the problem of outbreeding--the mixing of genes that are too different.

然而,与此同时,选择稍微相似基因的人为配偶也能避免远系繁殖差异太大的基因组合问题。

The I/D polymorphism of ACE was detected by polymerase chain reaction, and the CMA/B poly- morphism of Chymase gene was detected using PCR-restriction fragment length polymorphism.

运用聚合酶链反应检测 ACE基因的I/D多态性,运用限制性片段长度多态性技术检测Chymase基因的CMA/B多态性。

Meanwhile, we investigated the mechanisms by which HDAC inhibitor NaBu stimulates ALAS2 gene expression.

我们发现NaBu不仅能上调红系细胞ALAS2基因的转录,而且能激活非红系细胞ALAS2基因的转录。

Results The hyper-methylation rates of RASSF1A gene were 93.75%(15/16 cases) in NPCs and 0 in chronic nasopharyngitis.

结果 RASSF1A基因的高甲基化率在鼻咽癌组织中为93.75%(15/16),在慢性鼻咽炎组织中为,两组病例的RASSF1A基因高甲基化率差别显著,P.005。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法,同时,采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。

Hey Big Raven, The individual lies dont matter anymore - its ALL a tissue of lies in support of...

嘿大乌鸦,个别谎言的事不要再-其所有的组织的谎言,在支持。