基因的

During the postgenomics era the information about genomic sequence and gene functions provides a new foundation for evolutionary biology and ecology As the first whole-genome sequenced plant Arabidopsis thaliana and its wild relatives have played a critical role in understanding the evolution of genomics and speciation Both A halleri and A lyrata are closely related to the model species A thaliana A halleri ssp gemmifera occurs in northeastern China Japan and Taiwan; while its sister A halleri ssp halleri is mainly distributed in Europe Geographical barriers such as Tienshan Mountain Range isolate these intraspecific sisters Likewise A lyrata ssp kamchatica and ssp lyrata occur in East Asia and North America respectively Such distribution patterns seem to be consistent with allopartic speciation The comparison between ancestral and extant polymorphism by multilocus can be informative about the population genetics of speciation In this study we collected and analyzed DNA sequences of 98 genes from four wild relatives of A thaliana A halleri ssp gemmifera A halleri ssp halleri A lyrata ssp kamchatica and A lyrata ssp lyrata The ancestral states of these four species were compared to each other in terms of level of genetic variation However the ancestral species at the time of speciation were substantially more polymorphic than the extant geographical populations The observations are not fully compatible with speciation by strict allopatry At some species pairs parapatric speciation seems more reasonable in speciation of Arabidopsis The 98 gene sequences are also used for the congruence test between gene genealogy and species phylogeny Only 28 genes support the species phylogeny but there are 23 genes supports another major genealogy { lyrata} thaliana Based on the phylogenetic position change of A lyrata ssp kamchatica and Ks value for each species pair suggested the recent directional gene flow between A halleri ssp gemmifera and A lyrata ssp kamchatica

阿拉伯芥是第一个完成基因体定序的开花植物，其基因体资讯提供植物学研究的重要依据；在解析阿拉伯芥属物种的亲缘关系以及种化机制等重要的演化议题时，阿拉伯芥近缘的野生物种自然成了不可或缺的关键；跟阿拉伯芥近缘的物种包括A halleri及A lyrata，其中A halleri ssp gemmifera主要分布於中国东北、日本以及台湾，与近缘的A halleri ssp halleri其分布於欧洲隔著天山及大陆的障蔽，而A lyrata ssp kamchatica主要分布於东北亚及台湾，与分布於北美五大湖的A lyrata ssp lyrata被北极圈所分隔，这样的分布模式暗示异域种化的可能。藉由多基因分析比较祖先物种与现生物种遗传歧异度的相关可提供讯息探讨种化时期的族群遗传结构，本研究针对A halleri ssp gemmifera、A halleri ssp halleri及A lyrata ssp kamchatica、A lyrata ssp lyrata四个物种，两对互为亚种的姊妹群，以阿拉伯芥为外群进行研究，在四个物种完成98个同源基因的分子序列，利用套装软体MCMCcoal来估算祖先物种的遗传变异，亦估算现生物种的核苷酸歧异度，观察到？多物种配对中祖先物种遗传多型性大於现生物种DNA歧异度，显示异域种化模型并无法完全解释阿拉伯芥属物种的种化模式，在某些物种配对间邻域种化模式应比异域种化更为可能；在基因树与物种树的比较，98个基因片段的亲缘模式只有28个是与已知物种树一致的，有23个基因其树状图支持{ lyrata} thaliana的型式，藉由kamchatica位置的变化以及估算各物种配对间的平均同义置换率，推测在A halleri ssp gemmifera与A lyrata ssp kamchatica间具有近代的单方向基因交流。

The cowpea trypsin inhibitor gene and the bar gene discovered in recent years are found to be used for insect-resistant and anti -herbicide respectively because of their broad spectrum and are used in gene improvement of many crops widely now. Using the peanut Arachis hypogaea L. cv. Shanyou 523 and Arachis hypogaea L.

在多种抗植物虫害、耐除草剂基因中，豇豆蛋白酶抑制剂基因(cowpea trypsin inhibitor，CpTI)和耐除草剂基因因其抗虫和耐除草剂的广谱性而成为效果最为理想的外源基因，被广泛地应用于农作物抗虫、耐除草剂基因的改良上。

To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中，苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变，并筛选在自生生长时期、共生生长时期的表型，以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因，我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法，发现lsrA基因主要在根瘤侵染区开始表达，表达时序也在侵染阶段左右，但晚于bacA基因表达；LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体，nifA和fixK基因的转录水平降低，nifH基因的转录被完全阻断，因此LsrA蛋白为根瘤发育所必需，是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因，并研究了它的调节特性。oxyR突变后，苜蓿中华根瘤菌对过氧化氢敏感性提高，适应性降低。

The differentiation coefficient is 0.07 between three inbreed families respectively. The highest level of genetic homozygosity is in families Ⅱ at 18, but genetic variation is a little difference between inbreeding pig and mice. However, this study revealed the pattern of genetic variation at microsatellite loci in the 3 inbred families of WZSP with one of the most important findings to be the high level of unexpected heterozygosity at some loci, e.g. Sw874 and Sw936 that could be part of the unique genetics of three families. A hypothesis is that certain functional genes linked with such microsatellite loci may be involved in the maintenance of viability of these inbred families of WZSP remains for further investigation.

随近交代数的推进，每个星座上等位基因数越来越少，基因的纯合度越来越高，但WZSP近交系微卫星等位基因的纯合度有一定的限度，与近交鼠有所不同；在近交系3个家系各个世代中有少数几个基因座，如Sw874和Sw936等一直处于高度杂合状态，这可能与WZSP近交家系的种质特异性有关，推测与这些基因座处于同一连锁群的某些功能基因在维持极高近交水平下WZSP的基本生存能力中起着关键作用。3个近交群体间的分化系数在0.07以上，已各自成为独立家系；3个家系的近交程度不完全一致，基因的纯合度亦不同，Ⅱ系最高、14个微卫星座中7个纯合，其次为Ⅰ系和Ⅲ系。

Methods:The expressive plasmids of AML1b and AML1-ETO were transfected into CV-1 and 293 cells.The expression level of endogenous pig7 gene was detected by Realtime-PCR.The luciferase reporter plasmids containing pig7 enhancer/corresponding mutant sequences were constructed and co-transfected into CV-1 cells with expressive plasmids of AML1b and AML1-ETO.The transactivity of pig7 enhancer was assayed by luminometer.

研究方法：AML1b和AML7-ETO表达质粒分别转染及共转染CV-1和293细胞，用Realtime-PCR方法检测上述基因对两种细胞内源性pig7基因mRNA表达水平的影响；构建含AML1b结合位点的pig7基因增强子序列以及相对应的突变位点的荧光素酶报告基因质粒；将表达载体/突变载体与AML1b或AML1-ETO基因瞬时共转染CV-1细胞，分析AML1b、AML1-ETO对报告基因的转录调节作用。

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用实验一确定的PCR条件，以Hprt为阳性对照，用巢式RT-PCR对小鼠早期胚胎进行Xist基因的转录分析，结果发现，转录Sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录Xist基因；不转录Sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2-细胞期的过程中，Xist基因一直不转录，但是，从4-细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录Xist基因。

Studies of molecular genetics have demonstrated that retinoblastoma susceptibility gene is closely related to the development of retinoblastoma and the theory of Rb gene mutation or inactivation has been putatively proven to be the molecular mechanism of RB formation.

本研究以我国培养建立的一株RB细胞系〓及裸鼠为实验材料和对象，在对〓细胞抑癌基因（Rb基因、p53基因）表达状况进行检测和鉴定的基础上，通过建立裸鼠玻璃体腔RB常位异种移植模型，构建Rb基因的逆转录病毒表达载体，以脂质体介导的Rb基因转移方式，进行RB体内基因治疗的实验研究，观察Rb基因在体内对RB的治疗作用及影响因素，并探讨其作用机制。

In this study, genetic analyses were conducted to determine the genetic basis in an elite resistant inbred line Siyi with complete resistance to maize dwarf mosaic. A new genetic model, two dominant complementary genes conditioning the resistance, were found by Mendelian genetic analysis based on parents, F1, F2 and backcrosses in three successive years' field trails. The two genes were further mapped near the centromere of chromosome 3 and 6, respectively by tightly linked microsatellite markers using 242 plants from F2 generation. The resistance gene on chromosome 3 is 1.0 cM apart from the flanking markers phi053 and umc1527, respectively. Whereas the linkage distance between two flanking markers bnlg1600 and phi075 and resistance gene on chromosome 6 was 1.0 and 4.0 cM, respectively. Genotypic analysis of the plants from testcross and F3 populations supported the new genetic manners.

课题组通过连续三年的抗病鉴定，在国内种质资源中筛选出一份综合农艺性状优良、配合力较高的自交系四一，三年的表型遗传研究和两年的分子标记工作，发现四一中的玉米矮花叶病抗性是由两对显性互补基因控制的，进而利用F2作图群体，把发现的两个基因定位在第三和第六染色体的着丝点附近，并获得了双侧紧密连锁的分子标记连锁图谱，其中第三染色体上的分子标记UMC1527和phi053从抗病基因双侧逼近1 cM，而第六染色体上的分子标记phi075、bnlg1600从抗病基因双侧分别逼近4 cM和1 cM；利用B2群体、F2：3家系、BC3F1群体和带有第三、第六染色体抗病基因以及两个抗病基因的近等基因系，进一步证实了四一中成株期抗性是由两个显性互补基因控制的。

Objective The study was to detect gene expression profiles of leucocytes in schizophrenia using high throughput microarray analysis, in order to screen out susceptibility genes of schizophrenia.

精神分裂症易感基因的基因芯片筛查研究目的研究健康人及精神分裂症患者外周血白细胞基因表达谱，分析精神分裂症患者基因表达水平的变化，筛查精神分裂症易感基因。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。