基因的

Bottle for four hours, then collect RGCs and check the sum of apoptosis cells by flow cytometry.

在RGCs中加入DSX，加压培养后收集细胞，用基因芯片寻找凋亡基因的表达。

To construct recombinant adenovirus,the N and G genes were respectively cleaved from the pMD18-T plasmids by double restriction endonuclease,and then cloned into shuttle vector pAdTrack-CMV coinstantaneously.

经卡那霉素抗性和酶切筛选，最终成功构建了含有N基因和G基因的重组穿梭载体pAdTrack-CMV-N-G。

The recombinant eukaryotic expression vector pcDNA3.1-Myc-His B/tPA can stable express tPAcDNA in transfected hUVEC cell strains and can secret compt tPA protein. Experiment III The Effects of Transfected tPA Gene on Vascular Smooth Muscle Cells Proliferation and Migration in VitroObjective To investigate the effects of transfected tPA gene on vascular smooth muscle cell proliferation and migration in vitro and to provide a basis for further study on preventing vein graft restenosis.

结论构建的pcDNA3.1-Myc-HisB/tPA基因新型真核表达载体，能在HUVEC中表达，其表达具有高拷贝、稳定性好的特点，获得的表达蛋白活性较高，提示本载体和稳定表达tPA基因的人脐静脉内皮株能够作为基因治疗工具用于哺乳动物的实验研究。

Identification of two mutations in CTNS, the only gene known to be associated with cystinosis, is confirmatory.

CTNS基因是唯一已知的与胱氨酸病相关的基因，CTNS基因的两个突变的鉴定是被证实的。

A fusion gene of green fluorescent proteinand movement protein of cucumber mosaic viruswas obtained by using recombination-PCR.

采用重组-PCR方法，扩增获得绿色荧光蛋白与黄瓜花叶病毒运动蛋白融合基因，将其克隆到pKEN上，构建了该融合基因的原核表达载体pKENG-M。

OBJECTIVE: To investigate liposome-mediated cytosine deaminase gene transfecting rabbit BMSCs and its gene expression.

目的：探讨脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其基因的表达。

New strategies for breeding seedless plants have been proposed by using these genes. These include expression of some cytotoxin genes or genes for production of hormones in plants under the control of seed_coat_specific promoter or ovary_specific promoter and "terminator technology" etc. It's clear that these transgenic methods will be introduced into a wider range of fruit and vegetables in the future.

应用这些目的基因，已建立了转基因生产无籽果实的新策略，如在种皮或子房特异性启动子控制下的生长素基因或细胞毒素基因的表达及"终止子"技术的运用等，这将大规模地促进蔬菜和水果生产，提高果蔬产品的市场价值。

Subsequently, we carried through safty test, immunogenic test, efficacity test and preservation test of the vaccine.

成功地克隆、测序了猪细小病毒NS〓基因，并成功地实现了NS〓基因的体外表达。

Polymerase chain reaction is amplified and sequenced to determine the type of emm genes.

聚合酶链反应扩增并进行测序从而对emm基因进行分型确定emm基因的型别。

The fused insecticidal protein may delay the process of insects?developing resistance. After investigation of the secondary structure and action mode of Bt insecticidal protein and Cowpea trypsin proteinase inhibitor, as well as the action mechanism of insect enterokinase, we devised the fusion gene on the base of maintaining their functions respectively. The method follows: CpTI gene is linked to 3?-end of GFM crylA gene by a nucleotide sequence encoding cleavage site of insect enterokinase.

根据Bt杀虫蛋白Cry1A和豇豆胰蛋白酶抑制剂的高级结构、杀虫机理及昆虫体内肠激酶的作用机理，本着尽量不影响二者功能的原则，设计出了构建Cry1A和CpTI融合杀虫基因CryCI的方案，即将CpTI基因的编码序列连接到GFM Cry1A基因编码区3'端，并在其间用一段编码肠激酶切割位点的序列相连。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。