基因的

The common features of imprinted genes are, clustering of multiple imprinted genes in one chromosomal region (around 80%), conservation of imprinting among eutherian mammals, asynchrony of DNA replication of imprinted genes, temporal and spatial regulation of expression of imprinted genes, coding for untranslated RNAs as well as proteins, antisense transcripts may regulate expression of imprinted genes. Once established, the imprinting pattern is stably transmitted through cell division but reset in germ cells of the fetal gonads.

约80%的印记基因呈串出现在染色体上；在哺乳动物品种之间，印记基因具有较高的保守性；印记基因的复制通常表现为不同时性；一些印记基因具有印记遗传的时空性；少数印记基因只转录为mRNA而不翻译成蛋白质；印记基因的反意链通常表达，表达产生具有调节印记基因的作用。

The nucleoprotein gene and glycoprotein gene of rabies virus were amplified by RT-PCR and then cloned into pMD18-T plasmid,respectively.

将N基因和G基因分别克隆入质粒载体pMD18-T后，对筛选的含有N基因和G基因的重组质粒进行了全基因序列测定和分析。

The function of hypothesis transport genes NMB0098 and NMB0097 were tried to study by way of gene knock out. The phototype of N. meningitidis strain BT878 was observed before and after gene NMB0098 was knocked out.

选用质粒pUC18为目的基因的载体，经过酶切、去磷酸化、连接、电穿孔法转导和抗生素筛选等步骤，分别将目的基因NMB0098和NMB0097与质粒相连，并在目的基因中插入标记基因—卡那霉素基因，构建盒式插入的重组质粒pNB0098-K和pNB0097-K。

The method is simple, has safe operation, creates an approach utilizing a plant gene to safely select the marker gene for the tomato genetic transformation, can replace the disadvantages caused by the widely used heterogenous genes such as antibiotic genes, weedicide resistant genes, xyl A, pmi, GUS and so on, taken as marker genes, obtains a transgenic tomato with food safety, biological safety and ecological safety, relieves the public from anxiety of marker gene safety, and inevitably facilitates the commercialized application of the transgenic tomato.

该方法简单，操作安全，开创了利用植物基因作为番茄转化安全筛选标记基因的途径，能够代替目前广泛使用的抗生素基因、抗除草剂基因以及xylA、pmi、GUS等异源基因作为标记基因存在的弊端，获得具有食品安全性、生物安全性和生态安全性的转基因番茄，解除公众对标记基因安全性的顾虑，必将促进转基因番茄的商业化应用。

Long-distance PCR was used to amplify the β-actin gene for black carp based on the β-actin gene sequences of Cyprinidae. Construct of EGFP expression system containing 5'UTR of β-actin gene for black carp was transferred into zygotes of mud loach and COS-7 cells and the results showed that 5'UTR of β-actin gene for black carp could be used as the promoter of the"all-fish"transgene.

基于鲤科鱼类Beta-肌动蛋白基因序列，采用长片段PCR技术，克隆了青鱼β-actin基因DNA片段，并构建了青鱼β-actin基因5'启动调控区绿色荧光蛋白表达载体，通过显微注射泥鳅胚胎和COS-7细胞转染实验，证明了所克隆的青鱼β-actin基因5'启动调控区具有启动调控功能，并用之来构建&全鱼&基因的启动子部分。

Gene sequence was highly homologic with a gene encoding aβ- subunit of ATP synthetase and an EST originating from a cDNA in relation to water deficit stress, which both were closely associated with the protein functioning in stress resistance and ion transportation. The results suggested that No. 1 gene sequence was involved in stress resistance of apple rootstock. No. 2 gene sequence had high homology with a gene encoding a serine/ threonine kinase protein, indicating perhaps it was a new gene encoding the serine/ threonine kinase protein in apple rootstock. The gene sequences of A1 and A2 were highly homologic with a gene encoding aβsubunit of ATP synthetase.

对得到的差异片断进行回收、测序，通过BLAST和其它植物比对，发现Ⅰ号序列与一条编码ATP合酶β亚基基因和一条水分亏缺胁迫cDNA克隆基因EST的同源性非常高，该序列编码涉及到了抗性表达、离子转运基因，可能参与了植物抗性表达，说明获得的基因序列应是与苹果砧木抗性相关的基因；Ⅱ序列与一条serine/threonine kinase蛋白激酶的蛋白序列同源性比较高，核酸比对较低，可能是具有丝氨酸/苏氨酸蛋白激酶功能的新基因。A1、A2序列与一条编码ATP合酶β亚基基因的同源性较高。

The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

Results:(1) totally, the distributions of alleles o r genotypes of drd3 ser9gly polymorphism are not significantly different between the groups of paren ts, unaffected-siblings and affected-siblings, and between the sub-types of schi zophrenia.

结果： (1)总体而言，drd3基因的ser9gly等位基因频度和基因型频度在父母组、非患病同胞组和患病同胞组之间差异无显著性，在精神分裂症不同亚型之间基因型频度和等位基因频度的分布差异也没有统计学意义。

In this paper a 1 296 bp cDNA sequence was cloned from bamboo shoot of Phyllostachys violascens by RT-PCR method with degenerated primers based on the sequence conservation of Poaceae TB1 homologous genes.

进化分析进一步表明，竹子TB1相似基因是玉米TB1基因的同源基因，且竹子TB1同源基因的分歧时间介于水稻和现有其他TB1同源基因之间。

Phylogeny analysis indicates that the divergence time of bamboo TB1 homologs are later than rice OsTB1 but earlier than other Poaceae TB1 homologs.

进化分析进一步表明，竹子TB1相似基因是玉米TB1基因的同源基因，且竹子TB1同源基因的分歧时间介于水稻和现有其他TB1同源基因之间。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。