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The Sry gene sequence of Muntiacus crinifrons was acquired and the conclution was that there are 83% homology between the human and Muntiacus crinifrons. It was testified that in all mammal Sry gene is consertive. On the other side the Sry gene was located to the Y2 chromosome of the Muntiacus crinifrons.

最后提取雄性黑麂的基因组DNA,并用同一对引物对其进行扩增,亦得到Sry基因的片段,对此扩增片段进行克隆,测序,结果表明其与Y2染色体得到的Sry基因片段完全一样,与人SRY基因的同源性均为83%。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An.

目的 ①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异,阐明微小按蚊复合体亲缘种种间和种内变异;②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系,以确立微小按蚊亲缘种变异的种系地位;③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An. minimus A and C;③To establish specific methods for differentiation of two sibling species, An. minimus A and An. minimus C, of the Minimus Complex.

中文题名微小按蚊种群变异与分子鉴别研究副题名外文题名论文作者周水森导师汤林华研究员学科专业分子流行病学研究领域\研究方向学位级别博士学位授予单位中国预防医学科学院学位授予日期2002 论文页码总数98页关键词蚊微小按蚊馆藏号BSLW /2003 /R384 /3 目的①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异,阐明微小按蚊复合体亲缘种种间和种内变异;②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系,以确立微小按蚊亲缘种变异的种系地位;③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

The nonsynonymous SNPs located in protein functional sites or domains are pointed out, since they are more likely to affect the protein function than other nsSNPs and would be selected as target SNPs for association study.

VSD便利用了这个高度引用的和非冗余的资源,来重新编排精神分裂症相关基因的变异,将基因变异归于不同的基因位点,并以参考序列为标准在基因组、mRNAs和蛋白质三个水平上确定和定位变异位点。

The positive signal of ER and ER mRNA distributed in parenchymal and non-parenchymal hepatic cells, especially near the hepatic centrolobular and periportal areas. Ovariectomy decreased ER level and ER mRNA expression significantly (P<0.05). Hepatic ER and ER mRNA concentration were elevated after treatment with estrogen in both ovariectomy and sham ovariectomy fibrotic groups (P<0.05), and the elevation of ER was correlated with a marked decrease in hepatic damage and fibrosis.

5肝脏ERα及其mRNA基因的表达分布于肝细胞及肝脏非实质细胞,特别以肝脏中央静脉和门脉周围为主;卵巢切除减少ERα及其mRNA基因的表达(P<0.05);雌二醇治疗在肝纤维化和卵巢切除大鼠均提高肝脏ER及其mRNA基因的表达(P<0.05),其增加程度与肝脏组织学损伤、纤维化指标的降低程度以及肝脏胶原沉积减少相平行。

In order to understand the function of this gene within short time, we also constructed yeast (Schizosaccharomyces pombe vector to analyze the gene function, but the result investigated that over expression of this gene could not increase the length of yeast cell. It suggested that expression of the gene is not a direct reason in cell elongation.

为了在短时间内初步研究该基因的功能,构建了酵母表达载体,利用裂殖酵母表达系统对该基因的功能进行活体分析,没有发现该基因对单核的酵母细胞的伸长有明显影响。

We studied the mutation of PDS gene in children diagnosed to have prelingual non-syndromic hearing loss with concomitant bilateral large vestibular aqueduct and Mondini's dysplasia. We tried to find the correlation between non-syndromic hearing loss in local patients and PDS gene, and to establish the basic data of PDS gene mutations in Taiwan.

本篇研究之目的,乃对於患有非症候群学语前听障并合并双侧大前庭导水管及Mondini氏发育异常(Mondini's dysplasia)的儿童,作基因的分析,尝试找出本土非症候群听障与PDS基因的关连性,以建立台湾地区PDS基因突变的基本资料。

TUNEL method was used to detected the apoptosis in the artery. Results:①The MSC cocultured with VSMC expressed smooth muscle a-actin, calponin and CD31, no cells positive for calponin and CD31 were detected in the control group; and a lot of filaments were observed in the co-cultured MSC by electron microscopy.②We gain dual-stable expression of AT2R gene medicated by doxycycline regulatable system of mesenchymal stem cells.

单独培养及VSMC条件培养液培养的MSC仅SM-α-actin表达阳性;(2)Dox-on系统由四个质粒组成:调节质粒pUHD17-1 hyg,荧光报告质粒pUHC 13-3,筛选质粒pSV2neo以及含目的基因AT2R的应答质粒pUHD10-3/AT2R,以上质粒经鉴定均与其背景资料相符合,可用于实现目的基因片段在靶细胞中可调控表达研究;(3)本实验通过连续两个回合的转染及抗生素压力筛选,经Dox诱导表达后获取出低背景、高诱导表达AT2R基因的克隆。

By using this antisense MDR1 modified cell line, K562MDR/LASSN cells , we will further study the effects of MDR1 antisense RNA on the inhibition of MDR1 expression in drug-resistant leukemic cells and the resensitization of leukemic cells to anticancer agents.

希望通过逆转录病毒介导的MDR1基因转移从基因水平阻断MDR1基因的转录和翻译,增加肿瘤细胞对化疗药物的敏感性,从而达到杀灭肿瘤细胞的目的。

Torenia fournieri genomic DNA was digested with DraI、EcoRV、PvuII、SmaI respectively. A special adaptor was ligated to the ends of the digested DNA fragments as a template for adaptor PCR. With the adaptor and TfPLC1 gene-specific primers, bands of 798bp and 813bp upstream of TfPLC1 were obtained successively.

应用衔接头PCR技术,以蓝猪耳全基因组DNA分别经DraI、EcoRV、PvuII、SmaI消化后与衔接头连接产物为模板,用衔接头引物和TfPLC1基因的特异引物经过多轮的巢式PCR,先后克隆到两个大小为798bp、813bp的TfPLC1基因上游序列;经测序、blastn比较分析和拼接得到一个蓝猪耳TfPLC1基因的启动子序列,共1432bp。

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