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Transgenic plants expressing GNA showed significant insecticidal activity towards \\omoptera insects such as aphids and brown planthopper in bioassay and feeding tests.In this paper a lectin gene was firstly isolated from leaves of Zephyranthes grandiflora, belonging to Amaryllidaceae, by the use of rapid amplification of cDNA ends technique. Characterization, functional prediction and construction of plant and prokaryotic expression vector of this novel gene were studied.

本实验采用cDNA末端快速扩增(Rapid amplification of eDNA ends，RACE)技术，首次从中国特有的野生植物韭莲(又名风雨花，Zephyranthes grandiflora)的叶片中分离克隆出了一个新的凝集素基因，对基因进行了序列分析和功能预测，并成功地构建了韭莲凝集素基因的植物表达载体和原核表达载体。

The analysis of the mutants of amrA and amkA showed that the production of rifamycin SV had decreased about 10%-20%.

用双交换法在地中海拟无枝菌酸菌U32染色体上插入阿泊拉霉素抗性基因，成功地构建了了amrA，amkA和amrB基因的突变株，其中amrA，amkA基因突变株的菌丝形态与野生型U32之间有较明显的差别，在菌丝的末端形成卷曲或弯曲。

The cotton -δ-cadinene synthase (CAD1), a sesquiterpene cyclase, catalyzes a branch-point step leading to biosynthesis of sesquiterpene phytoalexins, including gossypol.

CAD1-A基因是CAD1基因家族的一个成员。在CAD1-A基因的启动子区存在一个可以与WRKY蛋白相结合的典型的W-box。

Results Multidrug resistance could be detected in the tumor tissue after the injection of retrovirus around and into it.After filtration and centrifugalization,the concentrated supernatant was 2 times efficient than the original supernatant and P-GP expression was detected in 60% of the tumor cells.mdr1 mRNA expression in the tumor tissue was significantly increased after gene transfer as detected by RT-PCR.

结果 携带mdr1基因的逆转录病毒上清液注射后肿瘤模型产生多药耐药性，经过滤和离心浓缩的病毒上清液60%细胞表达P-GP，是病毒原液基因转移率的2倍；RT-PCR表明基因转移后，肿瘤组织内mdr1 mRNA的表达量明显增加。

In a half diallel cross including 4 parents and 6 hybrids, Bt had significant effect on hybrids lint yield compared with those without Bt insertion.

对一套4×3半双列杂交组合以及2对近等基因系组合的遗传分析和抗虫性鉴定发现，杂交组合在没有Bt基因存在的情况下，Bt基因的引入对于杂交组合的抗虫性增强和产量增加有显著作用。

Sequencing analysis and database searches indicated that there were 19 known genes and 31 unknown cDNA fragments in the sequenced 72 dot blot positive clones specific for gastrula embryos,and 52 known genes and 37 unknown cDNA fragments in the sequenced 98 dot blot positive clones specific for tail bud embryos.

测序和基因数据库比对结果表明，72个原肠期斑点杂交阳性克隆中，包括19个已知基因的cDNA片段和31个没有同源性的cDNA片段；98个尾芽期斑点杂交阳性克隆中，包括52个已知基因的cDNA片段和37个没有同源性的cDNA片段。

One, the progress of the research of mankind of gene medicines and chemical reagents to oneself gene and gene project will be in will make pharmacy line of business obtains a leap in 10 years henceforth, remedial osteoporosis, early old sex fiber of sex of gawkish, bursa is out of shape the medicaments that waits for difficulty miscellaneous disease will come out, the cure of AIDS also will obtain a breakthrough.

一、基因药品人类对自身基因的研究和基因工程的进展将在今后10年中使制药业取得飞跃，治疗骨质疏松、早老性痴呆、囊性纤维变形等疑难杂症的药物将问世，艾滋病的治疗也将取得突破。

Inaddition to that,LFY has a second role in the activation of the floral homeotic genes that specify the identity of organs in the flower and is a key intergrator of the outputs of floral inductive pathways.DFL, a FLORICAULA/LEAFY homologous gene,which cloned from C.lavandulifolium,was a key gene of florescence for C.lavandulifolium.

LEAFY基因在植物成花过程中意义重大，它不仅决定着花由营养生长向生殖生长的转变，它还接连GA成花途径和长日途径的信号继而诱导花分生组织的转变，同时还是活化花器官基因的关键基因。

AIM: To find the susceptible or resistant genes of H pylori infectionin Bai-ethnic children of Kunming,and to explore the immunogenetic mechanism of H pylori infection.

目的：探讨昆明白族儿童幽门螺杆菌(Helicobacter pylori，H pylori)感染与人类白细胞抗原-DQA1(HLA-DQA1)等位基因的相关性，寻找是否存在与H pylori感染相关的易感基因或抵抗基因，从免疫学的角度探讨H pylori感染的免疫遗传机制。

Methods p53 codon 72 were genotyped with pcr-rflp technique for 156 zhuang healthily long-living people (aged 90～105, mean 93.2 years, longevous group) and 142 healthy controls (20～72, mean 34.8 years, control group) from bama area to analyze the polymorphic genotype frequencies.

应用pcr-rflp技术对巴马156例（年龄90～105岁，平均93.2岁，长寿组）健康壮族长寿老人和142例健康壮族成年人（年龄20～72岁，平均34.8岁，对照组）的p53基因进行分型，了解这些基因的多态性，分析该基因频率在长寿组和对照组之间的差异及其与长寿的关系。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。