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The concept of cell differentiation; tissue-specific genes; and house-keeping gene; combinational control arouse expression of tissue-specific gene; the factors which have influence on cell differentiation; totipotency; cell differentiation and embryo development.

细胞分化的基本概念;组织特异性基因与管家基因;组合调控引发组织特异性基因的表达;影响细胞分化的因素;细胞的全能性;细胞分化与胚胎发育。

Methylation of promoter in p16 gene is one of the important mechanisms of the inactivation of the gene.

喉癌中p16基因的甲基化是该基因失活的重要机制之一,p16基因的失活与喉癌的发展和演进密切相关。

The inactivation of p16 gene has a close relation with the development and progress of LSCC.

喉癌中p16基因的甲基化是该基因失活的重要机制之一,p16基因的失活与喉癌的发展和演进密切相关。

With regard to the rapidly increasing sequence data of prokaryotic genomes, accurate gene identifications and genome annotations have become more and more important.

近年来原核生物的基因体定序速度越来越快,相对地基因的鉴定以及基因的注解也变的越来越重要。

The gene regulating sequence of human collagen type Ⅰ is located at 5'end and first intron. The carriers of reporting gene was made, and the function of promoter, enhancer and inhibiter was examined in this study. On the basis of above work, MBP ectopic expression carriers were further built. The result laid a foundation for investigating the feasibility, and validity to specially express MBP gene of human nervous system, in tendon fibroblast.

人Ⅰ型胶原基因调节序列位于5'端和第一内含子,本研究构建了带报告基因的载体并检测了其启动子,增强子和减弱子的功能,以此为基础,进一步构建了MBP异位表达载体,为探索在神经系统少突胶质细胞特异表达的人脑髓鞘碱性蛋白MBP基因转至肌腱成纤维细胞表达的可行性和有效性奠定了基础。

G/ml. After wt-p53 transfection and VCR treatment, the number of Bel-7402 cells was decreased; the cells were swollen severely with irregular projections; pyknosis, karyorrhexis and karyolysis were observed. After wt-p53 transfection, the expression of mdr1 and P-gp in Bel-7402 cells was down-regulated remarkably, and the expression of TopoⅡα was up-regulated.

形态学检查转染细胞,在VCR作用下细胞数明显减少,细胞散在不成片、细胞高度水肿、胞体不规则突起,可见核固缩、核裂解、核溶解。p53基因对Bel-7402细胞的mdr1/P-gp的表达有明显的抑制作用,对TopⅡα基因的表达有上调作用,对GSTπ、MRP基因表达没有影响。

Molecular requirements for actin-based lamella formation in Drosophila S2 cells.

它的目的在于使感兴趣的单基因筛选以及为了研究多个基因的功能而进行的多基因平行筛选变得简单。

These data suggest that vaccination with DNA encoding amastin confers protectiveimmunity to mice infected with Leishmania donovani and the mice injected withpcDNA3.1-ctxB/amastin got stronger protective immunity.

结果表明:含amastin基因的两种DNA疫苗均可在小鼠体内产生一定的免疫保护效应,ctxB/amastin融合基因免疫组的免疫保护效果似乎强于单纯的amastin基因免疫组。

The vip3A genes amplified from wild-type Bt strains S184 and 611 with different levels of activity against lepidopteran insect larvae were cloned into pGEM-T Easy vector. Alignment of these 2 putative Vip3A proteins with 8 others showed that vip3A genes are highly conservative.

从以上菌株中挑选2个对夜蛾科害虫具有较高和较低毒力的菌株,即S184和611,分别进行vip3A基因的克隆和测序,再已报道的其它8个vip3A基因进行核苷酸和氨基酸序列比较,结果表明,vip3A是一个极其保守的基因。

We transformed antiapoptotic genes Bcl-2, CED-9 and PpBI-1 into the yeast to evaluate their function of resistance to cold stress. The results are as follows:(1) Cold induced apoptosis in yeast cells, moreover, yeast cells display representive features such as chromatin condensation, DNA fragmentation and chromatin margination.

而且酵母没有如Bcl-2家族的主要凋亡调控基因的同源基因,因此酵母是研究外源抗凋亡基因Bcl-2,CED-9和PpBI-1在抗冷方面的作用及其调控机制的理想体系。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

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